Bis-pyridinium quadrupolar derivatives.
High Stokes shift selective probes for bio-imaging

• Authors: Patrizio Salice , Silvia Versari , Silvia Bradamante , Francesco Meinardi , Giorgio Macchi , Giorgio A. Pagani , Luca Beverina
• Languages of publication: EN

Abstracts

EN

We describe the design, synthesis and characterization of
five high Stokes shift quadrupolar heteroaryl compounds suitable
as fluorescent probes in bio-imaging. In particular, we
characterize the photophysical properties and the intracellular
localization in Human Umbilical Vein Endothelial Cells
(HUVEC) and Human Mesenchymal Stem Cells (HMSCs) for
each dye. We show that, amongst all of the investigated
derivatives, the 2,5-bis[1-(4-N-methylpyridinium)ethen-2-yl)]-
N-methylpyrrole salt is the best candidates as selective mitochondrial
tracker. Finally, we recorded the full emission spectrum
of the most performing - exclusively mitochondrial selective
- fluorescent probe directly from HUVEC stained cells.
The emission spectrum collected from the stained mitochondria
shows a remarkably more pronounced vibronic structure
with respect to the emission of the free fluorophore in solution.

Keywords

EN

fluorescent probes ; imaging ; mitochondria ; heterocycles ; high Stokes Shift

• Publisher: De Gruyter Open
• Journal: Organic Photonics and Photovoltaics
• Year: 2013
• Volume: 1
• Pages: 39-55

Dates

online

05 - 11 - 2013

received

15 - 6 - 2013

accepted

17 - 7 - 2013

Contributors

author

Patrizio Salice

• Department of Materials Science and INSTM,
  University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy

author

Silvia Versari

• CNR-ISTM, Institute of Molecular Science and Technology,
  via Golgi 19, I-20133 Milan, Italy

author

Silvia Bradamante

• CNR-ISTM, Institute of Molecular Science and Technology,
  via Golgi 19, I-20133 Milan, Italy

author

Francesco Meinardi

• Department of Materials Science and INSTM,
  University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy

author

Giorgio Macchi

• Department of Materials Science and INSTM,
  University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy

author

Giorgio A. Pagani

• Department of Materials Science and INSTM,
  University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy

author

Luca Beverina

• Department of Materials Science and INSTM,
  University of Milano-Bicocca, Via Cozzi 53, I-20125,Milano, Italy

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Identifiers

DOI

10.2478/oph-2013-0005