Community Composition and Abundance of Protozoa Under Different Concentration Of Nitrogen Compounds at “Hajdow” Wastewater Treatment Plant / Zbiorowiska Pierwotniaków I Ich Liczebność W Oczyszczalni Ścieków „Hajdów” Przy Różnych Stężeniach Związków Azotu

• Authors: Katarzyna Jaromin-Gleń , Roman Babko , Grzegorz Łagód , Henryk Sobczuk
• Languages of publication: EN

Abstracts

EN

The protists notably ciliates and testate amoebas in the conditions of modernized wastewater treatment plants “Hajdow” (Lublin, Poland) and the nitrogen removal efficiency were studied. Sampling took place every week during the period of August-September 2010. The total of 31 taxa of ciliates and 3 taxa of testate amoebas were identified. Most of the species were registered during the whole research period. Significant abundance reached the following species: Acineria uncinata, Aspidisca cicada, Aspidisca lynceus, Vorticella aquadulcis, Vorticella infusionum, Arcella vulgaris, Euglypha acanthophora and Pyxidicula operculata. In September increase in efficiency of nitrogen removal process was registered. The research shows that the increase of nitrogen reduction efficiency was accompanied by increase of ciliates quantity, mainly due to peritrichid ciliates.

PL

Przedstawiono analizę zgrupowań pierwotniaków obejmującą orzęski oraz ameby skorupkowe, zasiedlające kolejne urządzenia głównego ciągu technologicznego oczyszczalni ścieków „Hajdów” na tle zmian w stężeniach związków azotu. Badania prowadzono po modernizacji części biologicznej realizowanej w celu podniesienia skuteczności usuwania ze ścieków związków biogennych, a w szczególności związków azotu. Pobór próbek prowadzony był raz w tygodniu w okresie sierpień-wrzesień 2010 roku. W badanym materiale biologicznym wyodrębniono 31 gatunków orzęsków oraz 3 gatunki ameb skorupkowych. Większość ze wspomnianych gatunków pierwotniaków odnotowywana była przez cały okres badań. Najbardziej licznie w analizowanym materiale biologicznym reprezentowane były następujące gatunki: Acineria uncinata, Aspidiscacicada, Aspidisca lynceus, Vorticella aquadulcis, Vorticella infusionum, Arcella vulgaris, Euglyphaacanthophora i Pyxidicula operculata. We wrześniu odnotowany został wzrost skuteczności procesu usuwania związków azotu. Prezentowane badania wskazują, iż wzrostowi stopnia usuwania ze ścieków związków azotu towarzyszył wzrost ilości orzęsków odnoszący się głównie do form peritricha.

Keywords

EN

activated sludge; wastewater treatment plants; sewage purification; ciliates; testate amoeba; nitrogen compounds

PL

osad czynny; oczyszczalnie ścieków; orzęski; ameby skorupkowe; związki azotu

• Publisher: De Gruyter Open
• Journal: Ecological Chemistry and Engineering S
• Year: 2013
• Volume: 20
• Issue: 1
• Pages: 127-139

Dates

published

1 - 03 - 2013

online

23 - 02 - 2013

Contributors

author

Katarzyna Jaromin-Gleń

• Institute of Agrophysics, Polish Academy of Sciences, ul. Doświadczalna 4, 20-290 Lublin, Poland, phone [phone]
• Faculty of Environmental Engineering, Lublin University of Technology, ul. Nadbystrzycka 40B, 20-618 Lublin, Poland, phone [phone],

author

Roman Babko

• Laboratory of Zoogeography, Department of Fauna and Systematic of Invertebrates, Schmalhausen Institute of Zoology NAS of Ukraine, B. Khmelnitsky 15 St., Kiev 01601, Ukraine

author

Grzegorz Łagód

• Faculty of Environmental Engineering, Lublin University of Technology, ul. Nadbystrzycka 40B, 20-618 Lublin, Poland, phone [phone],

author

Henryk Sobczuk

• Faculty of Environmental Engineering, Lublin University of Technology, ul. Nadbystrzycka 40B, 20-618 Lublin, Poland, phone [phone],

References

• [1] Kinner NE, Curds CR. Development of protozoan and metazoan communities in rotating biological contactor biofilms. Water Res. 1987;21(4):481-490. DOI: 10.1016/0043-1354(87)90197-7.[Crossref]
• [2] Lee NM, Welander T. Use of protozoa and metazoa for decreasing sludge production in aerobic wastewater treatment. Biotechnol Lett. 1996;18(4):429-434. DOI: 10.1007/BF00143465.[Crossref]
• [3] Selivanovskaya SY, Petrov AM, Egorova KV, Naumova RP. Protozoan and metazoan communities treating a simulated petrochemical industry wastewater in a rotating disc biological reactor. World J Microbiol and Biotechnol. 1997;13(5):511-517. DOI: 10.1023/A:1018505107030.[Crossref]
• [4] Martín-Cereceda M, Pérez-Uz B, Serrano S, Guinea A. Dynamics of protozoan and metazoan communities in a full scale wastewater treatment plant by rotating biological contactors. Microbiol Res. 2001;156(3):225-238. DOI: 10.1078/0944-5013-00105.[Crossref]
• [5] Madoni P. Estimates of ciliated protozoa biomass in activated sludge and biofilm. Bioresour Technol. 1994;48(3):245-249. DOI: 10.1016/0960-8524(94)90153-8.[Crossref]
• [6] Łagód G, Chomczyńska M, Montusiewicz A, Malicki J, Bieganowski A. Proposal of measurement and visualization methods for dominance structures in the saprobe communities. Ecol Chem Eng S. 2009;16(3):369-377.
• [7] Chomczyńska M, Montusiewicz A, Malicki J, Łagód G. Application of saprobes for bioindication of wastewater quality. Environ Eng Sci. 2009;26(2):289-295. DOI:10.1089/ees.2007.0311.[Crossref]
• [8] Jaromin K, Babko R, Łagód G. Abundances of protozoa on particular devices of "Hajdow" WWTP on the background of the nitrogen compounds concentration. Proc ECOpole. 2010;4(2):403-408.
• [9] Curds CR. The role of protozoa in the activated-sludge process. Amer Zool. 1973;13(1):161-169.
• [10] Arregui L, Serrano S, Linares M, Pérez-Uz B, Guinea A. Ciliate contributions to bioaggeregation: laboratory assays with axenic cultures of Tetrahymena thermophila. Internat Microbiol. 2007;10:91-96.
• [11] Arregui L, Linares M, Pérez-Uz B, Guinea A, Serrano S. Involvement of crawling and attached ciliates in the aggregation of particles in wasterwater treatment palnts. Air Soil Water Res. 2009;1:13-19.
• [12] Wilén BM, Lumley D, Mattsson A, Mino T. Relationship between floe composition and flocculation and settling properties studied at a full scale activated sludge plant. Water Res. 2008;42:4404-4418.[Crossref]
• [13] Curds CR. A theoretical study of factors influencing the microbial population dynamics of the activated-sludge process - I The effects of diurnal variations of sewage and carnivorous ciliated protozoa. Water Res. 1973;7(9):1269-1284.[Crossref]
• [14] Güde H. Grazing by protozoa as selection factor for activated sludge bacteria. Microbial Ecol. 1979;5:225-237. DOI: 10.1007/BF02013529.[Crossref]
• [15] Jürgens K, Pernthaler J, Schalla S, Amann R. Morphological and compositional changes in a planctonic bacterial community in response to enhanced protozoan grazing. Appl and Environ Microbiol. 1999;65(3):1241-1250.
• [16] Rønn R, McCaig AE, Griffiths BS, Prosser JI. Impact of protozoan grazing on bacterial community structure in soil microcosms. Appl and Environ Microbiol. 2002;68(12):6094-6105. DOI: 10.1128/AEM.68.12.6094-6105.2002.[PubMed][Crossref]
• [17] Curds CR, Cockburn A. Protozoa in biological sewage-treatment processes - II. protozoa as indicators in the activated-sludge process. Water Res. 1970;4(3):237-249.[Crossref]
• [18] Al-Shahwani SM, Horan NJ. The use of protozoa to indicate changes in the performance of activated sludge plants. Water Res. 1991;25(6):633-638. DOI: 10.1016/0043-1354(91)90038-R.[Crossref]
• [19] Madoni P. A sludge biotic index (SBI) for evaluation of activated sludge plants based on the microfauna analysis. Water Res. 1994;28:67-75. DOI: 10.1016/0043-1354(94)90120-1.[Crossref]
• [20] Martín-Cereceda M, Serrano S, Guinea A. A comparative study of ciliated protozoa communities in activated-sludge plants. FEMS Microbiol Ecol. 1996;21(4):267-276. DOI: 10.1111/j.1574-6941.1996.tb00123.x.[Crossref]
• [21] Salvado H, Gracia MP, Amigó JM. Capability of ciliated protozoa as indicators of effluent quality in activated sludge plants. Water Res. 1995;29(4):1041-1050. DOI: 10.1016/0043-1354(94)00258-9.[Crossref]
• [22] Eikelboom DH, van Buijsen HJJ. Microscopic Sludge Investigation Manual. 1st edition (in Polish). Szczecin: Sejdel-Przywecki; 1999.
• [23] Liwarska-Bizukojc E. Application of image analysis techniques in activated sludge wastewater treatment process. Biotechnol Lett. 2005;27:1427-1433. DOI: 10.1007/s10529-005-1303-2.[Crossref]
• [24] Hopkins BM. A quantitative image analysis system. Opt Eng. 1976;15:236-240.
• [25] Bieganowski A, Łagód G, Ryżak M, Montusiewicz A, Chomczyńska M, Sochan A. Measurement of activated sludge particle diameters using laser diffraction method. Ecol. Chem. Eng. S 2012;19(4):597-608. DOI: 10.2478/v10216-011-0042-7.[Crossref]
• [26] Kinner NE, Curds CR, Meeker LD. Protozoa and metazoan as indicators of treatment efficiency in rotating biological contactors. Water Sci Technol. 1989;20:199-2004.
• [27] McClure NC, Fry JC, Weightman AJ. Survival and catabolic activity of natural and genetically engineered bacteria in a laboratory-scale activated-sludge unit. Appl Environ Microbiol. 1991;57(2):366-373.
• [28] Salvadó H, Gracia MP. Determination of organic loading rate of activated sludge plants based on protozoan analysis. Water Res. 1993;27(5):891-895. DOI: 10.1016/0043-1354(93)90154-A.[Crossref]
• [29] Forney LJ, Liu WT, Guckert JB, Kumagai Y, Namkung E, Nishihara T, Larson RJ. Structure of microbial communities in activated sludge: potential implications for assessing the biodegradability of chemicals. Ecotoxicol and Environ Safety. 2001;49(1):40-53. DOI: 10.1006/eesa.2001.2034.[Crossref]
• [30] Lee S, Basu S, Tyler CW, Wei IW. Ciliate populations as bio-indicators at Deer Island treatment plant. Advances in Environ. Res. 2004;8:371-378. DOI: 10.1016/S1093-0191(02)00118-1.[Crossref]
• [31] Moussa MS, Hooijmans CM, Lubberding HJ, Gijzen HJ, van Loosdrecht MCM. Modelling nitrification, heterotrophic growth and predation in activated sludge. Water Res. 2005;39(20):5080-5098. DOI: 10.1016/j.watres.2005.09.038.[Crossref]
• [32] Ratsak CH, Maarsen KA, Kooijman SALM. Effects of protozoa on carbon mineralization in activated sludge. Water Res. 1996;30:1-12. DOI: 10.1016/0043-1354(95)00096-4.[Crossref]
• [33] Lee NM, Oleszkiewicz JA. Effects of predation and ORP conditions on the performance of nitrifiers in activated sludge systems. Water Res. 2003;37:4202-4210. DOI: 10.1016/S0043-1354(03)00341-5.[PubMed][Crossref]
• [34] Petropoulos P, Gilbride KA. Nitrification in activated sludge batch reactors is linked to protozoan grazing of the bacterial population. Can J Microbiol. 2005;51(9):791-799. DOI: 10.1139/W05-069.[Crossref][PubMed]
• [35] Gerardi MH. Wastewater Bacteria. New Jersey: John Wiley and Sons, Inc., Hoboken; 2006:77-101.
• [36] Pogue AJ, Gilbride KA. Impact of protozoan grazing on nitrification and the ammonia- and nitrite-oxidizing bacterial communities in activated sludge. Can J Microbiol. 2007;53(5):559-571. DOI: 10.1139/W07-027.[Crossref][PubMed]
• [37] Pérez-Uz B, Arregui L, Calvo P, Salvadó H, Fernández N, Rodrigeuz E, Zornaza A, Serrano S. Efficiency of nitrogen removal and protist communities: the potential for Introduction of novel biological index. Proc ofthe Int Wastewater Treatment, Monitoring and Reclamation: Key Role Played by the Waste Water Treatment Plant. ISRIM/ LIFE, 2009:1-9.
• [38] Pajdak-Stós A, Fiałkowska E, Fyda J, Babko R. Resistance of nitrifiers inhabiting activated sludge to ciliate grazing. Water Sci Technol. 2010;61(3):573-580. DOI: 10.2166/wst.2010.868.[Crossref]
• [39] Madoni P, Ghetti PF. The structure of Ciliated Protozoa communities in biological sewage-treatment plants. Hydrobiology 1981;83(2):207-215. DOI: 10.1007/BF00008268.[Crossref]
• [40] Stasinakis AS, Thomaidis N, Mamais D, Papanikolaou EC, Tsakon A, Lekkas TD. Effects of chromium (VI) addition on the activated sludge process. Water Res. 2003;37(9):2140-2148. DOI: 10.1016/S0043-1354(02)00623-1.[Crossref]
• [41] Madoni P, Davoli D, Chierici E. Comparative analysis of the activated sludge microfauna in several sewage treatment works. Water Res. 1993;27(9):1485-1491. DOI: 10.1016/0043-1354(93)90029-H.[Crossref]
• [42] Pérez-Uz B, Arregui L, Calvo P, Salvadó H, Fernández N, Rodrígeuz E, Zornoza A, Serrano S. Assessment of plausible bioindicators for plant performance in advanced wastewater treatment systems. Water Res. 2010;44:5059-5069. DOI: 10.1016/j.watres.2010.07.024.[Crossref]
• [43] Foissner W. Basic light and scanning electron microscopic methods for taxonomic studies of Ciliated Protozoa. Europ J Protistol. 1991;27:313-330.[Crossref]
• [44] Kahl A. Urtiere Oder Protozoa: Wimpertiere oder Ciliata (Infusoria). Die Tierwelt Deutschlands, Jena, G.Fischer, 18, 21, 25, 30, 1930-1935.
• [45] Stiller J. Szájkoszorús Csillósok-Peritricha. Fauna Hung. 1971;105:1-245.
• [46] Foissner W, Blatterer H, Berger H, Kohmann F. Taxonomische und okologische Revision der Ciliaten des Saprobiensystems - Band I: Cyrtophorida, Oligotrichida, Hypotrichida, Colpodea.- Informationsberichte des Bayer. Landesamtes fur Wasserwirtschaft. 1991;1(91):478-478.
• [47] Foissner W, Berger H, Kohmann F. Taxonomische und okologische Revision der Ciliaten des Saprobiensystems - Band II: Peritrichida, Heterotrichida, Odontostomatida.- Informationsberichte des Bayer. Landesamtes fur Wasserwirtschaft. 1992;5(92):502-502.
• [48] Foissner W., Berger H. and Kohmann F.: Taxonomische und okologische Revision der Ciliaten des Saprobiensystems - Band III: Hymenostomata, Prostomatida, Nassulida.- Informationsberichte des Bayer. Landesamtes fur Wasserwirtschaft. 1994;1(94):548-548.
• [49] Foissner W, Berger H, Blatterer H, Kohmann F. Taxonomische und okologische Revision der Ciliaten des Saprobiensystems - Band IV: Gymnostomatea, Loxodes, Suctoria.- Informationsberichte des Bayer. Landesamtes fur Wasserwirtschaft. 1995;1(95):540-540.
• [50] Foissner W, Berger H. A user-friendly guide to the ciliates (Protozoa, Ciliophora) commonly used by hydrobiologists as bioindicators in rivers, lakes, and waste waters, with notes on their ecology. Freshwater Biol. 1996;35:375-482.
• [51] Lee JJ, Leedale GF, Bradbury P. The illustrated guide to the protozoa. Society of Protozoologists, 2nd ed. Allen Press. Inc., 2000.
• [52] Adl SM, Simpson AGB, Farmer VA, Andersen RA, Anderson OR, Barta JR, et al. The new higher level classification of eukaryotes with emphasis on the taxonomy of protists. J Eukaryotic Microbiol. 2005;52(5):399-451. DOI: 10.1111/j.1550-7408.2005.00053.x.[Crossref]
• [53] Esteban G, Téllez C, Bautista LM. Dynamics of ciliated protozoa communities in activated-sludge process. Water Res. 1991;25:967-972. DOI: 10.1016/0043-1354(91)90145-G.[Crossref]
• [54] Babko R, Łagód G, Jaromin-Gleń KM. Abundance and structure of ciliated protozoa community at the particular devices of "Hajdów" WWTP. Ann. Set Environ. Protect. 2012;14:56-68.
• [55] Babko RV, Kuzmina TM. Ciliata (Protista, Ciliophora) of Epiphyton of Higher Aquatic Plants in a Small River. Hydrobiol J. 2004;40(4):22-38.

Identifiers

DOI

10.2478/eces-2013-0010