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2015 | 17 | 3 | 84-88

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Studies on the production of glucose isomerase by Bacillus licheniformis


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This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis. The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1% glycerol (specific activity = 6.88 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity = 5.24 U/mg protein). Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Best enzyme activity was observed in the presence of Mg2+ (specific activity = 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity = 1.02 U/mg protein). The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50ºC.









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1 - 9 - 2015
19 - 9 - 2015


  • University of Nigeria, Industrial Microbiology and Biotechnology Laboratory, Department of Microbiology, Nsukka, Nigeria


  • 1. Bhosale, S.H., Rao, M.B. & Deshpande, V.V. (1996). Molecular and industrial aspects of glucose isomerase. Microbiol. Rev. 60, 280–300. PMID 8801434.
  • 2. Priya, C. & Virendra, S.B. (2000). Application of a compatible xylose isomerase in simultaneous bioconversion of glucose and xylose to ethanol. Biotechnol. Bioproc. Engineering 5, 32–39. DOI: 10.1007/BFO2932350.[Crossref]
  • 3. Silva, E.A.B, Souza, A.A.U., Rodrigues, A.E. & Guelli, S.M.A. (2006). Glucose isomerisation in simulated moving bed reactor by glucose isomerase. Braz. Arch Biol Technol. 49: 491–502. [Crossref]
  • 4. Lawal, A.K., Banjoko, A.M., Banjoko, A.M., Osikoyia, A.F., Olatope, S.O., Kayode, O.F., Etoamihe, M., Emoleila., I., Alebiosu, F.A., Majolagbe, Y.L, Shittu, K.A, Buhari, F. & Dike, E.N. (2012). Production and optimal performance studies of glucose isomerase from agriculture raw material Global Adv. Res. J. Microbiol. 1(7), 108–119.
  • 5. Angardi, V. & Calik, P. (2013). Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3). J. Chem. Technol. Biotechnol. 88(5), 845–852. DOI: 10.1002/jctb.3910.[Crossref][WoS]
  • 6. Akdag, B. & Calik, P. (2014). Recombinant protein production by sucrose-utilizing Escherichia coli W: untreated beet molasses-based feeding strategy development. J. Chem. Technol. Biotechnol. DOI: 10.1002/jctb.4411.[Crossref]
  • 7. Ata, O., Boy, E., Gunes, H. & Calik, P. (2014). Codon optimization of xylA gene for recombinant glucose isomerase production in Pichia pastoris and fed-batch feeding strategies to fine-tune bioreactor performance. Bioproc. Biosyst. Engineering. DOI: 10.1007/s00449-014-1333.[Crossref][WoS]
  • 8. Dhungel, B., Subedi, M., Tiwari, K.B., Shrestha, U.T., Pokhrel, S. & Agrawal, V.P. (2007). The thermostable glucose isomerase from psychrotolerant Streptomyces species. Int. J. Life Sci 1, 6–10. DOI: 10.3126/ijls.v1i0.2300.[Crossref]
  • 9. Lee, C. & Zeikus, J.G. (1991). Purification and characterization of thermostable glucose isomerase from Clostridium thermosulfurogenes and Thermoanaerobacter strain B6A. Biochem. J. 274, 565–571.
  • 10. Bray, G.A., Nielsen, S.J. & Popken, B.M. (2004). Consumption of high – fructose corn syrup in beverages may play a role in the epidemic of obesity. Am. J.Clin. Nutr. 79, 537–543. PMID 15051594.
  • 11. Gromada, A., Fiedurek, J. & Szczodrak, J. (2008). Isoglucose production from raw strachy materials based on a two-stage enzymatic system. Pol. J. Microbiol. 57(2), 141–148.
  • 12. Gaily, M.H., Sulieman, A.K. & Abasaeed, A.E. (2013). Kinetics of a three – step isomerization of glucose to fructose using immobilized enzyme. International J. Chem. Engine. Applic. 4(1), 31–34. DOI:10.7763/IJCEA.2013.V4.255.[Crossref]
  • 13. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley J.T. & Williams, S.T. (1994). Bergey’s Manual of Determinative Bacteriology (9th ed.) Baltimore: Williams and Wilkins.
  • 14. Kulka, R.G. (1956). Colorimetric estimation of ketopentoses and ketohexoses. Biochem J. 63, 542–548. PMID 13355847.
  • 15. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951). Protein measurement with folin- phenol reagent. J. Biochem. 193, 265–275. PMID 14907713.
  • 16. Deshmukh, S.S., Deshpande, M.V. & Shankar, V. (1994). Medium optimization for the production of glucose isomerase from thermophilic Streptommsces thermonitrificans. World J. Microbiol. Biotechnol. 10, 264–267. Doi: 10.1007/BF00414859.[Crossref]
  • 17. Chou, C.C., Ladisch, M.R. & Tsao, G.T. (1976). Studies on glucose isomerase from Streptomyces species. Appl. Environ. Microbiol. 32(4), 489–493. PMID 984827.
  • 18. Chen, W.P., Anderson A.W. & Han Y.W. (1979). Purification of glucose isomerase by Streptomyces flarogriseus. Appl. Environ. Microbiol. 37, 324–331. PMID 16345347.
  • 19. Wong, H.C., Ting, Y., Lin, H.C., Reichert, F. & Myambo, K. (1991). Genetic organization and regulation of the xylose degradation genes in Streptomyces rubiginosus. J. Bacteriol. 173, 6849–6858. PMID 1657868.
  • 20. Kwakman, J.H.J.M. & Postma, P.W. (1994). Glucose kinase has a regulatory role in catabolite repression in Streptomyces coelicor. J. Bacteriol. 176, 2694–2698. PMID 8169219.
  • 21. Yassien, M.A.M. & Jiman-Fatani, A.A.M. (2012). Optimization of glucose isomerase production by Streptomyces albaduncus. Afr. J. Microbiol. Res. 6, 2976–2984. DOI:10.5897/AJMR12.016.[Crossref]
  • 22. Whitlow, M., Howard, A.J., Finzel, B.C., Poulos, T.L., Winborne, E. & Gilliland, G.L. (1991). A metal-mediated hydride shift mechanism for xylose isomerase based on the 1.6A Streptomyces rubiginosus structures with xylitol and D-xylose. Proteins 9, 153–173. DOI: 10.1002/prot.340090302.[Crossref]
  • 23. Kasumi T., Hayashi, K. & Tsumura, N. (1980). Purification and enzymatic properties of glucose isomerase from Streptomyces griseofuscus S-41. Agric. Biol. Chem. 45, 1087–1095.
  • 24. Ryu, D.Y., Chung, S.H. & Katoh, K. (1977). Performance of the continuous glucose isomerase reactor systems for the production of fructose syrup. Biotechnol. Bioeng. 19, 159–184. DOI: 10.1002/bit.260190202.[Crossref]
  • 25. Kitada, M., Dobshi, Y. & Horikoshi, K. (1989). Enzymatic properties of purified D-xylose isomerase from a thermophilic alkalophile Bacillus TX-3. Agric Biol. Chem. 53(6), 1461–1468. DOI: 10.1271/bbb1961.53.1461.[Crossref]
  • 26. Danno, G.L. (1970). Studies on D-glucose-isomerizing enzyme from Bacillus coagulans strain HN-68. Agric. Biol. Chem. 34, 1805–1814.

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