Studies on the production of glucose isomerase by Bacillus licheniformis

• Authors: Ogbonnaya Nwokoro
• Languages of publication: EN

Abstracts

EN

This work reports the effects of some culture conditions on the production of glucose isomerase by Bacillus licheniformis. The bacterium was selected based on the release of 3.62 mg/mL fructose from the fermentation of glucose. Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in a medium containing 0.5% xylose and 1% glycerol (specific activity = 6.88 U/mg protein). Media containing only xylose or glucose gave lower enzyme productivies (specific activities= 4.60 and 2.35 U/mg protein respectively). The effects of nitrogen substrates on glucose isomerase production showed that yeast extract supported maximum enzyme activity (specific activity = 5.24 U/mg protein). Lowest enzyme activity was observed with sodium trioxonitrate (specific activity = 2.44 U/mg protein). In general, organic nitrogen substrates supported higher enzyme productivity than inorganic nitrogen substrates. Best enzyme activity was observed in the presence of Mg2+ (specific activity = 6.85 U/mg protein) while Hg2+ was inhibitory (specific activity = 1.02 U/mg protein). The optimum pH for best enzyme activity was 6.0 while optimum temperature for enzyme production was 50ºC.

Keywords

EN

glucose isomerase; carbon sources; nitrogen sources; metal ions

• Publisher: De Gruyter Open
• Journal: Polish Journal of Chemical Technology
• Year: 2015
• Volume: 17
• Issue: 3
• Pages: 84-88

Dates

published

1 - 9 - 2015

online

19 - 9 - 2015

Contributors

author

Ogbonnaya Nwokoro

• University of Nigeria, Industrial Microbiology and Biotechnology Laboratory, Department of Microbiology, Nsukka, Nigeria

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Identifiers

DOI

10.1515/pjct-2015-0054