PL EN


Preferences help
enabled [disable] Abstract
Number of results
2015 | 2 | 1 |
Article title

Integrated optical biosensor for rapid detection of
bacteria

Content
Title variants
Languages of publication
EN
Abstracts
EN
In medical diagnostics, rapid detection of
pathogenic bacteria from body fluids is one of the basic issues.
Most state-of-the-art methods require optical labeling,
increasing the complexity, duration and cost of the
analysis. Therefore, there is a strong need for developing
selective sensory devices based on label-free techniques,
in order to increase the speed, and reduce the cost of detection.
In a recent paper, we have shown that an integrated
optical Mach-Zehnder interferometer, a highly sensitive
all-optical device made of a cheap photopolymer, can be
used as a powerful lab-on-a-chip tool for specific, labelfree
detection of proteins. By proper modifications of this
technique, our interferometric biosensor was combined
with a microfluidic system allowing the rapid and specific
detection of bacteria from solutions, having the surface of
the sensor functionalized by bacterium-specific antibodies.
The experiments proved that the biosensor was able
to detect Escherichia coli bacteria at concentrations of 106
cfu/ml within a few minutes, that makes our device an appropriate
tool for fast, label-free detection of bacteria from
body fluids such as urine or sputum. On the other hand,
possible applications of the device may not be restricted to
medical microbiology, since bacterial identification is an
important task in microbial forensics, criminal investigations,
bio-terrorism threats and in environmental studies,
as well.
Publisher

Year
Volume
2
Issue
1
Physical description
Dates
received
1 - 6 - 2015
accepted
20 - 10 - 2015
online
31 - 12 - 2015
Contributors
author
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
author
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
author
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
author
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
author
  • Institute of Medical Microbiology,
    Semmelweis University, Budapest, Hungary
author
  • Institute of Biophysics,
    Biological Research Centre of the Hungarian Academy of Sciences,
    Szeged, Hungary
References
  • [1] D.N. Gilbert, B. Spellberg, J.G. Bartlett, An Unmet Medical Need:Rapid Molecular Diagnostics Tests for Respiratory Tract Infections,Clinical Infectious Diseases 52(S4), 2011, 384
  • [2] I. Abubakar, L. Irvine, C.F. Aldus, G.M. Wyatt, R. Fordham,S. Schelenz, L. Shepstone, A. Howe, M. Peck, P.R. Hunter,A systematic review of the clinical, public health and costeffectivenessof rapid diagnostic tests for the detection andidentification of bacterial intestinal pathogens in faeces andfood Health Technol. Assess. 11, 2007, 36
  • [3] A. Dér, S. Valkai, A. Mathesz, I. Andó, E.K. Wolff, P. Ormos,Protein-based all-optical sensor device, Sensors and ActuatorsB 151, 2010, 26
  • [4] A. Dér, L. Fábián, S. Valkai, J.J. Ramsden, P. Ormos, E.K. Wolff,Integrated optical switching based on the protein bacteriorhodopsin,Photochem. Photobiol. 83, 2007, 393[WoS][Crossref]
  • [5] K. Tiefenthaler, W. Lukosz, Sensitivity of grating couples as integratedoptical biosensors, J. Opt. Soc. Am. B 6, 1989, 209[Crossref]
  • [6] J. Vörös, J.J. Ramsden, G. Csúcs, I. Szendrő, S.M. De Paul, M.Textor, N.D. Spencer, Optical grating coupler biosensors, Biomaterials23, 2002, 3699[Crossref]
  • [7] P. Galajda, P. Ormos, Complex micromachines produced anddriven by light, Appl. Phys. Lett. 78, 2001, 249[Crossref]
  • [8] L. Bissonnette, M.G. Bergeron, Infectious DiseaseManagementthrough Point-of-Care Personalized Medicine Molecular DiagnosticTechnologies, J. Pers. Med. 2, 2012, 50[Crossref]
  • [9] J.J. Ramsden,S.-Y. Li, E. Heinzle, J.E. Prenosil, Optical methodfor measurement of number and shape of attached cells in realtime, Cytometry 19, 1995, 97[Crossref]
  • [10] R. Horváth, H.C. Pedersen, N. Skivesen, D. Selmeczi, N.B.Larsen, Optical waveguide sensor for on-line monitoring of bacteria,Optics Lett. 28 (2003) 1233-1235
  • [11] M. Miravitlles, A. Marín, E. Monsó, S. Vilŕ, C. de la Roza,R. Hervás, C. Esquinas, M. García, L. Millares, J. Morera, A. Torres,Colour of sputum is a marker for bacterial colonisationin chronic obstructive pulmonary disease, Respiratory Res. 11,2010, 58[WoS]
  • [12] M.L. Wilson, L. Gaido, Laboratory Diagnosis of Urinary Tract Infectionsin Adult Patients, Medical Microbiol. 38, 2004, 1150
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_optof-2015-0002
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.