Integrated optical biosensor for rapid detection of
bacteria

• Authors: Anna Mathesz , Sándor Valkai , Attila Újvárosy , Badri Aekbote , Orsolya Sipos , Balázs Stercz , Béla Kocsis , Dóra Szabó , András Dér
• Languages of publication: EN

Abstracts

EN

In medical diagnostics, rapid detection of
pathogenic bacteria from body fluids is one of the basic issues.
Most state-of-the-art methods require optical labeling,
increasing the complexity, duration and cost of the
analysis. Therefore, there is a strong need for developing
selective sensory devices based on label-free techniques,
in order to increase the speed, and reduce the cost of detection.
In a recent paper, we have shown that an integrated
optical Mach-Zehnder interferometer, a highly sensitive
all-optical device made of a cheap photopolymer, can be
used as a powerful lab-on-a-chip tool for specific, labelfree
detection of proteins. By proper modifications of this
technique, our interferometric biosensor was combined
with a microfluidic system allowing the rapid and specific
detection of bacteria from solutions, having the surface of
the sensor functionalized by bacterium-specific antibodies.
The experiments proved that the biosensor was able
to detect Escherichia coli bacteria at concentrations of 106
cfu/ml within a few minutes, that makes our device an appropriate
tool for fast, label-free detection of bacteria from
body fluids such as urine or sputum. On the other hand,
possible applications of the device may not be restricted to
medical microbiology, since bacterial identification is an
important task in microbial forensics, criminal investigations,
bio-terrorism threats and in environmental studies,
as well.

Keywords

EN

Biosensor; Integrated optics; Mach-Zehnder interferometer; microfluidics; E. coli; bacteria

• Publisher: De Gruyter Open
• Journal: Optofluidics, Microfluidics and Nanofluidics
• Year: 2015
• Volume: 2
• Issue: 1

Dates

received

1 - 6 - 2015

accepted

20 - 10 - 2015

online

31 - 12 - 2015

Contributors

author

Anna Mathesz

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

author

Sándor Valkai

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

author

Attila Újvárosy

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

author

Badri Aekbote

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

author

Orsolya Sipos

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

author

Balázs Stercz

• Institute of Medical Microbiology,
  Semmelweis University, Budapest, Hungary

author

Béla Kocsis

• Institute of Medical Microbiology,
  Semmelweis University, Budapest, Hungary

author

Dóra Szabó

• Institute of Medical Microbiology,
  Semmelweis University, Budapest, Hungary

author

András Dér

• Institute of Biophysics,
  Biological Research Centre of the Hungarian Academy of Sciences,
  Szeged, Hungary

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Identifiers

DOI

10.1515/optof-2015-0002