In this work, we demonstrate the unique
capability of surface imprinted hydrogels over magnetic
nanoparticles (Fe3O4@MIP-NPs) for protection of
enzyme proteins from denaturation induced by heat,
UV irradiation, ultrasonication and addition of urea.
Moreover, the Fe3O4@MIP-NPs successfully enabled
rapid renaturation of heat-denatured proteins under
mild conditions. These interesting properties of MIP
nanoparticles offer useful tools for protection and
renaturation of proteins in various applications.