Magnetic surface imprinted hydrogel
nanoparticles for specific and reversible
stabilization of proteins

• Authors: Yibin Liu , Junqiu Zhai , Jiantong Dong , Meiping Zhao
• Languages of publication: EN

Abstracts

EN

In this work, we demonstrate the unique
capability of surface imprinted hydrogels over magnetic
nanoparticles (Fe3O4@MIP-NPs) for protection of
enzyme proteins from denaturation induced by heat,
UV irradiation, ultrasonication and addition of urea.
Moreover, the Fe3O4@MIP-NPs successfully enabled
rapid renaturation of heat-denatured proteins under
mild conditions. These interesting properties of MIP
nanoparticles offer useful tools for protection and
renaturation of proteins in various applications.

Keywords

EN

Surface imprinted hydrogel; Magneticnanoparticles; Selective binding; Protein protection; Renaturation

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2014
• Volume: 2
• Issue: 1

Dates

online

11 - 2 - 2016

received

14 - 8 - 2015

accepted

23 - 10 - 2015

Contributors

author

Yibin Liu

• Beijing National Laboratory
  for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
  and Molecular Engineering, College of Chemistry and Molecular
  Engineering, Peking University, Beijing 100871, China

author

Junqiu Zhai

• Beijing National Laboratory
  for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
  and Molecular Engineering, College of Chemistry and Molecular
  Engineering, Peking University, Beijing 100871, China

author

Jiantong Dong

• Beijing National Laboratory
  for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
  and Molecular Engineering, College of Chemistry and Molecular
  Engineering, Peking University, Beijing 100871, China

author

Meiping Zhao

• Beijing National Laboratory
  for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
  and Molecular Engineering, College of Chemistry and Molecular
  Engineering, Peking University, Beijing 100871, China

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Identifiers

DOI

10.1515/molim-2015-0006