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2014 | 2 | 1 |

Article title

Magnetic surface imprinted hydrogel
nanoparticles for specific and reversible
stabilization of proteins

Content

Title variants

Languages of publication

EN

Abstracts

EN
In this work, we demonstrate the unique
capability of surface imprinted hydrogels over magnetic
nanoparticles (Fe3O4@MIP-NPs) for protection of
enzyme proteins from denaturation induced by heat,
UV irradiation, ultrasonication and addition of urea.
Moreover, the Fe3O4@MIP-NPs successfully enabled
rapid renaturation of heat-denatured proteins under
mild conditions. These interesting properties of MIP
nanoparticles offer useful tools for protection and
renaturation of proteins in various applications.

Publisher

Year

Volume

2

Issue

1

Physical description

Dates

online
11 - 2 - 2016
received
14 - 8 - 2015
accepted
23 - 10 - 2015

Contributors

author
  • Beijing National Laboratory
    for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
    and Molecular Engineering, College of Chemistry and Molecular
    Engineering, Peking University, Beijing 100871, China
author
  • Beijing National Laboratory
    for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
    and Molecular Engineering, College of Chemistry and Molecular
    Engineering, Peking University, Beijing 100871, China
author
  • Beijing National Laboratory
    for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
    and Molecular Engineering, College of Chemistry and Molecular
    Engineering, Peking University, Beijing 100871, China
author
  • Beijing National Laboratory
    for Molecular Sciences, MOE Key Laboratory of Bioorganic Chemistry
    and Molecular Engineering, College of Chemistry and Molecular
    Engineering, Peking University, Beijing 100871, China

References

  • [1] Coleman, D. & Howitt, F.O. Denaturation and Renaturation ofProteins. Nature, 1945, 155, 78-79.
  • [2] Luse, R.A. & McLaren, A.D. Mechanism of Enzyme Inactivationby Ultraviolet Light and the Photochemistry of Amino Acids (at2537 Å)*. Photochem Photobiol, 1963, 2, 343-360.
  • [3] Seiji, M. & Itakura, H. Enzyme Inactivation by UltravioletIrradiation and Protective Effect of Melanin in vitro1. J InvestDermatol, 1966, 47, 507-511.
  • [4] Lacks, S.A., Springhorn, S.S. & Rosenthal, A.L. Effect of thecomposition of sodium dodecyl sulfate preparations on therenaturation of enzymes after polyacrylamide gel electrophoresis.Anal Biochem, 1979, 100, 357-363.[WoS]
  • [5] Zhang, Y.L., Pan, X.M. & Zhou, J.M. Surface hydrophobicity andthermal aggregation of adenylate kinase. Biochem Mol Biol Int,1998, 44, 949-960.
  • [6] Gianfreda, L. & Scarfi, M.R. Enzyme stabilization: state of theart. Mol Cellular Biochem, 1991, 100, 97-128.
  • [7] Johnson, G.R. & Luckarift, H.R. Enzyme stabilization viabio-templated silicification reactions. Meth Mol Biol, 2011, 679,85-97.
  • [8] Iyer, P.V. & Ananthanarayan, L. Enzyme stability andstabilization-Aqueous and non-aqueous environment. ProcessBiochem, 2008, 43, 1019-1032.[WoS]
  • [9] Hwang, E.T. & Gu, M.B. Enzyme stabilization by nano/microsized hybrid materials. Eng Life Sci, 2013, 13, 49-61.[WoS]
  • [10] Wulff, G. Molecular Imprinting In Cross-Linked Materials withthe Aid Of Molecular Templates - a Way Towards ArtificialAntibodies. Angew Chem Int Edit, 1995, 34, 1812-1832.
  • [11] Wang, S., Li, D., Hua, Z. & Zhao, M. Molecularly imprintedmonolith coupled on-line with high performance liquidchromatography for simultaneous quantitative determinationof cyromazine and melamine. Analyst, 2011, 136, 3672-367.
  • [12] Haupt, K. & Mosbach, K. Molecularly imprinted polymersand their use in biomimetic sensors. Chem Rev, 2000, 100,2495-2504.
  • [13] Eersels, K. et al. Heat-transfer-method-based cell culturequality assay through cell detection by surface imprintedpolymers. Langmuir, 2015, 31, 2043-2050.[WoS]
  • [14] Schirhagl, R., Latif, U., Podlipna, D., Blumenstock, H. & Dickert,F.L. Natural and biomimetic materials for the detection ofinsulin. Anal Chem, 2012, 84, 3908-3913.[WoS]
  • [15] Servant, A., Haupt, K. & Resmini, M. Tuning molecularrecognition in water-soluble nanogels with enzyme-like activityfor the kemp elimination. Chemistry, 2011, 17, 11052-11059.[WoS]
  • [16] Caldorera-Moore, M. & Peppas, N.A. Micro- and nanotechnologiesfor intelligent and responsive biomaterial-basedmedical systems. Adv Drug Deliver Rev, 2009, 61, 1391-1401.[WoS]
  • [17] Piletska, E.V. et al. Magnetic high throughput screening systemfor the development of nano-sized molecularly imprintedpolymers for controlled delivery of curcumin. Analyst, 2015,140, 3113-3120.[WoS]
  • [18] Beierle, J.M. et al. Polymer nanoparticle hydrogels withautonomous affinity switching for the protection of proteinsfrom thermal stress. Angewandte Chemie, 2014, 53,9275-9279.[WoS]
  • [19] Barati, R. et al. Polyelectrolyte complex nanoparticles forprotection and delayed release of enzymes in alkaline pH andat elevated temperature during hydraulic fracturing of oil wells.J Appl Polymer Sci, 2012, 126, 587-592.
  • [20] Liu, Y. et al. Enhancing the selectivity of enzyme detectionby using tailor-made nanoparticles. Anal Chem, 2013, 85,4853-4857.[WoS]
  • [21] Hua, Z., Chen, Z., Li, Y. & Zhao, M. Thermosensitive andsalt-sensitive molecularly imprinted hydrogel for bovine serumalbumin. Langmuir, 2008, 24, 5773-5780.[WoS]
  • [22] Jiang, K.Y. et al. Protein immobilization on carbon nanotubesvia a two-step process of diimide-activated amidation. J MaterChem, 2004, 14, 37-39.
  • [23] Forde, J. et al. Chemical modification and immobilisationof laccase from Trametes hirsuta and from Myceliophtorathermophila. Enzyme Microb Tech, 2010, 47, 241-241.[WoS]
  • [24] Ganguli, S. et al. Regulation of Lysozyme Activity Based onThermotolerant Protein/Smart Polymer Complex Formation. JAm Chem Soc, 2009, 131, 6549-6553.[WoS]
  • [25] Rozema, D. & Gellman, S.H. Artificial Chaperones - ProteinRefolding Via Sequential Use of Detergent and Cyclodextrin. JAm Chem Soc, 1995, 117, 2373-2374.
  • [26] Lacks, S.A. & Springhorn, S.S. Renaturation of enzymes afterpolyacrylamide gel electrophoresis in the presence of sodiumdodecyl sulfate. J Biol Chem, 1980, 255, 7467-7473.
  • [27] Rosenthal, A.L. & Lacks, S.A. The Effect of the Compositionof Sds Preparations on the Renaturation of Various Enzymesafter Polyacrylamide-Gel Electrophoresis. Fed Proc, 1980, 39,1851-1851.
  • [28] Chen, Z. et al. Protein-responsive imprinted polymers withspecific shrinking and rebinding. JMR, 2008, 21, 71-77.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_1515_molim-2015-0006
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