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2014 | 2 | 1 |
Article title

Selective adsorption of trypsin using molecularly
imprinted polymers prepared with PEG-based
hydrogels containing anionic functional
monomers

Content
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EN
Abstracts
EN
Molecularly imprinting (MI) hydrogels for
selective adsorption of trypsin are reported. The trypsin
imprinted hydrogels were prepared using a polyethylene
glycol (PEG)-based dimethacrylate as a crosslinker and
anionic functional monomers. The hydrogel prepared
without any functional monomers showed significantly
low ability to adsorb a variety of proteins. We optimized
the concentration and the length of PEG units of the
crosslinkers to achieve the complete removal of the template
molecule and suitable selective adsorption. Additionally,
the functional monomers chosen were anionic since the
template, trypsin, is a basic protein. The adsorption tests
for proteins, done on the prepared MI gels, indicated
that the MI gel prepared with sodium allyl sulfonate (AS)
as a functional monomer showed much higher selective
adsorption for trypsin, even though a mixture of trypsin
and cytochrome c was used as the protein solution. The
selective adsorption was more effective in a NaCl solution
in which the non-specific adsorption by a sulfonate is
suppressed, similarly to our findings in a previous study.
The MI gel prepared with acrylic acid also showed the
selectivity, although the adsorption strength was lower
than that of the MI gel containing AS. We believe that the
present study constitutes the first approach for the selective
adsorption of trypsin using PEG-based hydrogels.
Publisher
Year
Volume
2
Issue
1
Physical description
Dates
online
11 - 2 - 2016
received
31 - 7 - 2015
accepted
8 - 8 - 2015
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_molim-2015-0004
Identifiers
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