Selective adsorption of trypsin using molecularly
imprinted polymers prepared with PEG-based
hydrogels containing anionic functional
monomers

• Authors: Takuya Kubo , Shunsuke Arimura , Toyohiro Naito , Koji Otsuka
• Languages of publication: EN

Abstracts

EN

Molecularly imprinting (MI) hydrogels for
selective adsorption of trypsin are reported. The trypsin
imprinted hydrogels were prepared using a polyethylene
glycol (PEG)-based dimethacrylate as a crosslinker and
anionic functional monomers. The hydrogel prepared
without any functional monomers showed significantly
low ability to adsorb a variety of proteins. We optimized
the concentration and the length of PEG units of the
crosslinkers to achieve the complete removal of the template
molecule and suitable selective adsorption. Additionally,
the functional monomers chosen were anionic since the
template, trypsin, is a basic protein. The adsorption tests
for proteins, done on the prepared MI gels, indicated
that the MI gel prepared with sodium allyl sulfonate (AS)
as a functional monomer showed much higher selective
adsorption for trypsin, even though a mixture of trypsin
and cytochrome c was used as the protein solution. The
selective adsorption was more effective in a NaCl solution
in which the non-specific adsorption by a sulfonate is
suppressed, similarly to our findings in a previous study.
The MI gel prepared with acrylic acid also showed the
selectivity, although the adsorption strength was lower
than that of the MI gel containing AS. We believe that the
present study constitutes the first approach for the selective
adsorption of trypsin using PEG-based hydrogels.

Keywords

EN

molecularly imprinting; polyethylene glycol; trypsin; anionic monomer

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2014
• Volume: 2
• Issue: 1

Dates

online

11 - 2 - 2016

received

31 - 7 - 2015

accepted

8 - 8 - 2015

Contributors

author

Takuya Kubo

• Graduate School of
  Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
  Japan

author

Shunsuke Arimura

• Graduate School of
  Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
  Japan

author

Toyohiro Naito

• Graduate School of
  Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
  Japan

author

Koji Otsuka

• Graduate School of
  Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
  Japan

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Identifiers

DOI

10.1515/molim-2015-0004