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2014 | 2 | 1 |

Article title

Selective adsorption of trypsin using molecularly
imprinted polymers prepared with PEG-based
hydrogels containing anionic functional
monomers

Content

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Languages of publication

EN

Abstracts

EN
Molecularly imprinting (MI) hydrogels for
selective adsorption of trypsin are reported. The trypsin
imprinted hydrogels were prepared using a polyethylene
glycol (PEG)-based dimethacrylate as a crosslinker and
anionic functional monomers. The hydrogel prepared
without any functional monomers showed significantly
low ability to adsorb a variety of proteins. We optimized
the concentration and the length of PEG units of the
crosslinkers to achieve the complete removal of the template
molecule and suitable selective adsorption. Additionally,
the functional monomers chosen were anionic since the
template, trypsin, is a basic protein. The adsorption tests
for proteins, done on the prepared MI gels, indicated
that the MI gel prepared with sodium allyl sulfonate (AS)
as a functional monomer showed much higher selective
adsorption for trypsin, even though a mixture of trypsin
and cytochrome c was used as the protein solution. The
selective adsorption was more effective in a NaCl solution
in which the non-specific adsorption by a sulfonate is
suppressed, similarly to our findings in a previous study.
The MI gel prepared with acrylic acid also showed the
selectivity, although the adsorption strength was lower
than that of the MI gel containing AS. We believe that the
present study constitutes the first approach for the selective
adsorption of trypsin using PEG-based hydrogels.

Publisher

Year

Volume

2

Issue

1

Physical description

Dates

online
11 - 2 - 2016
received
31 - 7 - 2015
accepted
8 - 8 - 2015

Contributors

author
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan
author
  • Graduate School of
    Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510,
    Japan

References

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Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_1515_molim-2015-0004
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