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2015 | 28 | 1 | 17-20
Article title

The alteration in peripheral neutrophils of patients with chronic kidney disease

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EN
Abstracts
EN
Recent findings have demonstrated the impaired functions of neutrophils of patients with chronic renal failure. The purpose of our research was to study oxidative modified proteins, as well as the histone spectrum in neutrophils drawn from patients with chronic kidney disease, and to estimate the ability of neutrophils to form spontaneous neutrophil extracellular traps. In this work, we have assumed that metabolic alteration in neutrophils may develop at early stages of chronic kidney disease. Materials and methods: Neutrophils obtained from patients with various stages of chronic kidney disease and degrees of chronic renal failure were used. As control, blood samples obtained from 32 healthy individuals was employed. In the examined neutrophils, advanced oxidation protein products, protein reactive carbonyl derivatives, as well as nucleosomal histones were detected. The ability of neutrophils to form spontaneous neutrophil extracellular traps was estimated. Our results have demonstrated an increase of nucleosomal histones in neutrophils of all patients with chronic kidney disease. Moreover, our work fixes the rate of growth of intracellular advanced oxidation protein products and the decreasing of protein reactive carbonyl derivatives in neutrophils from patients with chronic kidney disease. Furthermore, we demonstrate the presence of the neutrophils with altered oxidative status and the decomposition of the histone spectrum in the circulation of patients with chronic kidney disease.
Publisher

Year
Volume
28
Issue
1
Pages
17-20
Physical description
Dates
published
1 - 3 - 2015
accepted
16 - 2 - 2015
received
7 - 1 - 2015
online
9 - 5 - 2015
Contributors
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan, lem2403@mail.ru
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
  • Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan
References
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  • 3. Dolgushin I., Andreeva Y.: Method for detecting neutrophils extracellular traps: Patent of Russia Federation. № 2384844, 2008
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  • 7. Levine R.L. et al.: Determination of carbonyl content in oxidatively modified proteins. Method. Enzymol, 186, 1990.
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  • 12. Davison A.M., Cameron J.S., Grunfeld J.-P., et.al. Editors (2005). Oxford Textbook of Clinical Nephrology. 3rd Ed. New York, Oxford University Press.
  • 13. Parker H. et al.: Myeloperoxidase associated with neutrophil extracellular traps is active and mediates bacterial killing in the presence of hydrogen peroxide. Journal of Leukocyte Biology, 91, N 3, 2012.[WoS][Crossref]
  • 14. Sharma A. et al.: Impaired function of neutrophils in uraemic patients. Natl Med J India, 3, 2000.
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  • 16. Zawrotniak M., Rapala-Kozik M.: Neutrophil extracellular traps (NETs) - formation and implications. Acta Biochim. Pol., 60, (3), 2013.
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_cipms-2015-0034
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