The alteration in peripheral neutrophils of patients with chronic kidney disease

• Authors: Larissa Yevgenyevna Muravlyova , Vilen Borisovich Molotov-Luchankiy , Ryszhan Yemelyevna Bakirova , Anar Akylbekovna Turmukhambetova , Dmitriy Anatolyevich Klyuyev , Ludmila Andreevna Demidchik , Yevgeniya Alexandrovna Kolesnikova
• Languages of publication: EN

Abstracts

EN

Recent findings have demonstrated the impaired functions of neutrophils of patients with chronic renal failure. The purpose of our research was to study oxidative modified proteins, as well as the histone spectrum in neutrophils drawn from patients with chronic kidney disease, and to estimate the ability of neutrophils to form spontaneous neutrophil extracellular traps. In this work, we have assumed that metabolic alteration in neutrophils may develop at early stages of chronic kidney disease. Materials and methods: Neutrophils obtained from patients with various stages of chronic kidney disease and degrees of chronic renal failure were used. As control, blood samples obtained from 32 healthy individuals was employed. In the examined neutrophils, advanced oxidation protein products, protein reactive carbonyl derivatives, as well as nucleosomal histones were detected. The ability of neutrophils to form spontaneous neutrophil extracellular traps was estimated. Our results have demonstrated an increase of nucleosomal histones in neutrophils of all patients with chronic kidney disease. Moreover, our work fixes the rate of growth of intracellular advanced oxidation protein products and the decreasing of protein reactive carbonyl derivatives in neutrophils from patients with chronic kidney disease. Furthermore, we demonstrate the presence of the neutrophils with altered oxidative status and the decomposition of the histone spectrum in the circulation of patients with chronic kidney disease.

Keywords

EN

neutrophils; oxidative proteins; histones; neutrophils extracellular traps chronic kidney disease

• Publisher: De Gruyter Open
• Journal: Current Issues in Pharmacy and Medical Sciences
• Year: 2015
• Volume: 28
• Issue: 1
• Pages: 17-20

Dates

published

1 - 3 - 2015

accepted

16 - 2 - 2015

received

7 - 1 - 2015

online

9 - 5 - 2015

Contributors

author

Larissa Yevgenyevna Muravlyova

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Vilen Borisovich Molotov-Luchankiy

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Ryszhan Yemelyevna Bakirova

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Anar Akylbekovna Turmukhambetova

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Dmitriy Anatolyevich Klyuyev

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Ludmila Andreevna Demidchik

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

author

Yevgeniya Alexandrovna Kolesnikova

• Karaganda State Medical University, Gogol Street 40, 100008 Karaganda, Kazakhstan

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Identifiers

DOI

10.1515/cipms-2015-0034