PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2015 | 13 | 1 |
Article title

Sterilization of bacteria suspensions and identification of radicals deposited during
plasma treatment

Content
Title variants
Languages of publication
EN
Abstracts
EN
In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.
Publisher

Journal
Year
Volume
13
Issue
1
Physical description
Dates
online
26 - 11 - 2014
accepted
29 - 5 - 2014
received
31 - 1 - 2014
Contributors
author
  • Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia
author
  • Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia
  • Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia
  • Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia
  • Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia
  • Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia
  • Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia
  • Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia
  • Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia
  • Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia
References
  • [1] Cheruthezhekatt S., Černak M., Slaviček P., Havel J., J. Appl. Biomed., 2010, 8, 55[Crossref]
  • [2] Shintani H., Sakudo A., Burke P., McDonnell G., Exp. Ther. Med., 2010, 1, 731
  • [3] Kim C.H. et al., J. Biotechnol., 2010, 150, 530
  • [4] Hong Y.F. et al., Lett. Appl. Microbiol., 2009, 48, 33[Crossref]
  • [5] Lee K., Peak K.H., Ju W.T., Lee Y., J. Microbiol., 2006, 44, 269
  • [6] Yu H. et al., J. Appl. Microbiol., 2006, 101, 1323[Crossref]
  • [7] Cotter J.J. et al., J. Hosp. Infect., 2011, 78, 204[Crossref]
  • [8] Maisch T. et al., Plos one, 2012, 7, e34610
  • [9] Jiang C. et al., ISRN Dentistry, 2012, 2012, Article ID 295736
  • [10] Stoffels E., Flikweert A.J., Stoffels W.W., Kroesen G.M.W., Plasma Sources Sci. T., 2002, 11, 383[Crossref]
  • [11] Puač N. et al., J. Phys. D. Appl. Phys., 2006, 39, 3514[Crossref]
  • [12] Malović G., Puač N., Lazović S., Petrović Z., Plasma Sources Sci. T., 2010, 19, 034014[Crossref]
  • [13] Jackson S.K., Liu K.J., Liu M., Timmins G.S., Free Radical Bio. Med., 2002, 32, 228[Crossref]
  • [14] Stoll S., Schweiger A., J. Magn. Reson., 2006, 178, 42
  • [15] Stoffels E. et al., Plasma Sources Sci. Technol., 2007, 16, 549[Crossref]
  • [16] Frejaville C. et al., J. Med. Chem. 1995, 38, 258[Crossref]
  • [17] Karoui H., Chalier F., Finet J.P., Tordo P., Org. Biomol. Chem., 2011, 9, 2473[Crossref]
  • [18] Fujii S., Yoshimura T., Coordin. Chem. Rev., 2000, 198, 89
  • [19] Ermolaeva S.A. et al., J. Med. Microbiol., 2011, 60, 75
  • [20] Lazović S. et al. New J. Phys., 2010, 12, 083037
  • [21] Ghaffari A., Miller C.C., McMullin B., Ghahary A., Nitric oxide, 2006, 14, 21 [Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_chem-2015-0041
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.