Sterilization of bacteria suspensions and identification of radicals deposited during
plasma treatment

• Authors: Nevena Puač , Maja Miletić , Miloš Mojović , Ana Popović-Bijelić , Dragana Vuković , Biljana Miličić , Dejan Maletić , Saša Lazović , Gordana Malović , Zoran Lj. Petrović
• Languages of publication: EN

Abstracts

EN

In this paper we will present results for plasma sterilization of planktonic samples of two reference strains of bacteria, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. We have used a plasma needle as a source of non-equilibrium atmospheric plasma in all treatments. This device is already well characterized by OES, derivative probes and mass spectrometry. It was shown that power delivered to the plasma is bellow 2 W and that it produces the main radical oxygen and nitrogen species believed to be responsible for the sterilization process. Here we will only present results obtained by electron paramagnetic resonance which was used to detect the OH, H and NO species. Treatment time and power delivered to the plasma were found to have the strongest influence on sterilization. In all cases we have observed a reduction of several orders of magnitude in the concentration of bacteria and for the longest treatment time complete eradication. A more efficient sterilization was achieved in the case of gram negative bacteria.

Keywords

EN

plasma needle ; sterilization; spin-trap; Electron Paramagnetic Resonance; radicals

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2015
• Volume: 13
• Issue: 1

Dates

online

26 - 11 - 2014

accepted

29 - 5 - 2014

received

31 - 1 - 2014

Contributors

author

Nevena Puač

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

author

Maja Miletić

• Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia

author

Miloš Mojović

• Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia

author

Ana Popović-Bijelić

• Faculty of Physical Chemistry, University of Belgrade, University of Belgrade, 11000 Belgrade, Serbia

author

Dragana Vuković

• Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia

author

Biljana Miličić

• Faculty of Dental Medicine, University of Belgrade, 11000 Belgrade, Serbia

author

Dejan Maletić

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

author

Saša Lazović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

author

Gordana Malović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

author

Zoran Lj. Petrović

• Institute of Physics, University of Belgrade, 11080 Belgrade, Serbia

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Identifiers

DOI

10.1515/chem-2015-0041