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Article title

Cross-linked enzyme aggregates (CLEA) in enzyme
improvement – a review


Title variants

Languages of publication



Structural and functional catalytic
characteristics of cross-linked enzyme aggregates
(CLEA) are reviewed. Firstly, advantages of enzyme
immobilization and existing types of immobilization
are described. Then, a wide description of the factors
that modify CLEA activity, selectivity and stability is
presented. Nowadays CLEA offers an economic, simple
and easy tool to reuse biocatalysts, improving their
catalytic properties and stability. This immobilization
methodology has been widely and satisfactorily tested
with a great variety of enzymes and has demonstrated
its potential as a future tool to optimize biocatalytic








Physical description


17 - 9 - 2015
25 - 11 - 2015
25 - 2 - 2016


  • Heterogeneous
    Biocatalysis group, CIC Biomagune, Parque Tecnológico de San
    Sebastián, Edificio Empresarial “C”, Paseo Miramón 182, 20009,
    Donostia-San Sebastián Guipúzcoa, Spain
  • Departamento de
    Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av.
    San Rafael Atlixco #186, Col. Vicentina 09340, D.F. México
  • Heterogeneous
    Biocatalysis group, CIC Biomagune, Parque Tecnológico de San
    Sebastián, Edificio Empresarial “C”, Paseo Miramón 182, 20009,
    Donostia-San Sebastián Guipúzcoa, Spain
  • Ikerbasque, Basque Foundation for Science,
    48011, Bilbao, Spain
  • Centro de Investigación y Asistencia en
    Tecnología y Diseño del Estado de Jalisco, A.C., Unidad de Biotecnología
    Industrial, Guadalajara, México
  • Departamento de
    Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av.
    San Rafael Atlixco #186, Col. Vicentina 09340, D.F. México


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