Cross-linked enzyme aggregates (CLEA) in enzyme
improvement – a review

• Authors: Susana Velasco-Lozano , Fernando López-Gallego , Juan C. Mateos-Díaz , Ernesto Favela-Torres
• Languages of publication: EN

Abstracts

EN

Structural and functional catalytic
characteristics of cross-linked enzyme aggregates
(CLEA) are reviewed. Firstly, advantages of enzyme
immobilization and existing types of immobilization
are described. Then, a wide description of the factors
that modify CLEA activity, selectivity and stability is
presented. Nowadays CLEA offers an economic, simple
and easy tool to reuse biocatalysts, improving their
catalytic properties and stability. This immobilization
methodology has been widely and satisfactorily tested
with a great variety of enzymes and has demonstrated
its potential as a future tool to optimize biocatalytic
processes.

Keywords

EN

cross-linking; enzyme; immobilization; stabilization

• Publisher: De Gruyter Open
• Journal: Biocatalysis
• Year: 2014
• Volume: 1
• Issue: 1

Dates

received

17 - 9 - 2015

accepted

25 - 11 - 2015

online

25 - 2 - 2016

Contributors

author

Susana Velasco-Lozano

• Heterogeneous
  Biocatalysis group, CIC Biomagune, Parque Tecnológico de San
  Sebastián, Edificio Empresarial “C”, Paseo Miramón 182, 20009,
  Donostia-San Sebastián Guipúzcoa, Spain
• Departamento de
  Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av.
  San Rafael Atlixco #186, Col. Vicentina 09340, D.F. México

author

Fernando López-Gallego

• Heterogeneous
  Biocatalysis group, CIC Biomagune, Parque Tecnológico de San
  Sebastián, Edificio Empresarial “C”, Paseo Miramón 182, 20009,
  Donostia-San Sebastián Guipúzcoa, Spain
• Ikerbasque, Basque Foundation for Science,
  48011, Bilbao, Spain

author

Juan C. Mateos-Díaz

• Centro de Investigación y Asistencia en
  Tecnología y Diseño del Estado de Jalisco, A.C., Unidad de Biotecnología
  Industrial, Guadalajara, México

author

Ernesto Favela-Torres

• Departamento de
  Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa. Av.
  San Rafael Atlixco #186, Col. Vicentina 09340, D.F. México

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Identifiers

DOI

10.1515/boca-2015-0012