Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 18

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  SECALE CEREALE
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Saturating rye genetic map with amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD) markers

100%
Bednarek P. T., Masojc P., Lewandowska R., Myskow B.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 1
21-33
EN
A linkage map of rye, previously developed using DS2 ? RXL10 F2 mapping population, was enriched with 179 AFLP and 19 RAPD marker loci. The current map covers 1386 cM and contains 480 markers including 200 RFLPs, 179 AFLPs, 88 RAPDs, 12 protein loci and one dwarfing gene. AFLPs generated by EcoRI/MseI primer combinations were distributed over the entire genome as distinct loci or clusters of 2-14 tightly linked DNA fragments. New marker loci mapped distally to the existing framework, significantly increased coverage of chromosomes 1R, 2R and 5R. The average marker distance is now 2.9 cM, but in seven regions the closest markers are still more than 20 cM apart. A detailed description of the newly mapped AFLP and RAPD loci is presented. The relationship with other published rye maps is discussed.
2

Characteristics of somaclonal variation in rye (Secale cereale L.)

100%
Rakoczy-Trojanowska M.
Biotechnologia
|
2002
|
issue 2
175-183
EN
As it was shown in numerous works concerning various plant species tissue culture derived plants may serve as potential variability resources for breeding. This subject was also investigated in rye. However, the number of publications is relatively low. A somaclonal variation in rye was investigated on different levels as molecular one, cytological or morphological. Based on these searches, several general conclusions can be drawn: (1) the frequency of somaclonal changes in rye is high, which can be connected with the presence of genome region, especially susceptible for tissue culture conditions, (2) the range and spectrum of somaclonal changes depend on donor plant genotype, (3) the majority of somaclonal changes has an negative character, e.g. reduction of plant viability, fertility or yield quality influence negatively plant quality, (4) some somaclonal changes can be useful for rye breeding.
3

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

100%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 1
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
4

Mapping QTLs for -amylase activity in rye grain

100%
Masojc P., Milczarski P.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 2
115-123
EN
Genetic control of alpha-amylase activity in rye grain was investigated by QTL mapping based on DS2 ? RXL10 intercross consisting of 99 F5-6 families propagated at one location during four vegetation seasons. A wide range of variation in -amylase activity and transgression effects were found among families and parental lines. This variation was shown to be determined in 40.1% by 7 significant (LOD score not less than 2.5) and 2 putative QTLs (2 < LOD < 2.5) distributed on all rye chromosomes except 4R. Two significant QTLs located on 3RL and 5RL chromosome arms were expressed each year. The third significant QTL was detected in three years (1RL). The other four significant QTLs (2RL, 5RS, 6RL, 7RL) were found in one year of study. The number and composition of QTLs were specific for a given year varying from three to six. QTLs were not correlated with isoenzyme polymorphisms at the structural alpha-Amy1 loci. A QTL associated with a region containing the alpha-Amy3 locus was detected on chromosome 5RL. Both high- and low-activity QTL alleles were found in each parental line, which explains the appearance of transgressive recombinants in the segregating population.
5

Rye SCAR markers for male fertility restoration in the P cytoplasm are also applicable to marker-assisted selection in the C cytoplasm

100%
Stojalowski S., Jaciubek M., Masojc P.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 4
371-373
EN
The study aimed at testing the usefulness of recently developed SCAR markers on rye (Secale cereale L.) chromosome 4R in hybrid breeding based on the C source of male sterility-inducing cytoplasm. Of 10 markers studied, 4 revealed polymorphisms between 2 inbred lines (544cms-C and Ot0-20) crossed to develop F2 and BC1 mapping populations. Analyses performed on 94 F2 and 93 BC1 plants allowed to extend a formerly constructed genetic map of chromosome arm 4RL. Three SCAR markers (SCP14M55, SCP15M55 and SCP16M58) were mapped in the vicinity of gene Rfc1, which restores male fertility in the C cytoplasm. The 3 tested SCAR markers proved to be effective in marker-assisted selection (MAS) for male fertility/sterility.
6

New genetic map of rye composed of PCR-based molecular markers and its alignment with the reference map of the DS2 ? RXL10 intercross

100%
Milczarski P., Banek-Tabor A., Lebiecka K., Stojalowski S., Myskow B., Masojc P.
Journal of Applied Genetics
|
2007
|
vol. 48
|
issue 3
11-24
EN
A new genetic map of rye, developed by using the 541 ? Ot1-3 F2 intercross, consists of 148 marker loci, including 99 RAPDs, 18 SSRs, 14 STSs, 9 SCARs and 7 ISSRs, and spans the distance of 1401.4 cM. To the 7 rye chromosomes, 8 linkage groups were assigned and compared with the reference map of the DS2 ? RXL10 F2 intercross by using 24 common markers. The 2 combined maps contain altogether 611 marker loci (70?109 per chromosome) and constitute a substantial source of information useful for further genomic studies in rye. From 21 to 37 RAPD marker loci are distributed randomly along each chromosome length and their total number for all 7 rye chromosomes is 177. This abundance of RAPD marker loci in the rye genetic map can be exploited for development of SCARs in regions containing important genes or QTL.
7

Transfer of genes from Dasypyrum villosum (Haynaldia villosa L.) to Secale cereale L.

100%
Apolinarska B., Gruszecka D.
Biotechnologia
|
2001
|
issue 2
63-65
EN
The subject of the study was fertile diploid hybrids of rye, cv. Amilo (2n = RR = 14) with Dasypyrum villosum (Crimea, Ukraine) (2n = VV = 14) ? wild species belonging to Triticeae. translocation. Plants which morphologicaly resembled rye and which showed the highest The analysed strains were obtained using in vitro cultures with subsequent backcrossing of hybrid plants with pollen of ?Amilo? or D. villosum in the case of heterozygotic fertility and total protein content in kernels were selected during several generations. The small differences in the occurrence of heterochromatine bands in rye chromosomes were revealed by the differential staining technique at the stage of mitosis, between hybrid plants and ?Amilo? cv. Cells with mixoploid chromosome number 2n = 13, 2n = 14 and 2n = 15 were present in some plants. Neither substitution nor evident chromosome translocation were found in generations B3/F3 and B4/F2 under investigation. Therefore, the technique of nucleic acids hybridization in situ will be applied to detect fragments of D. villosum genotype introgressed into rye.
8

Genetic polymorphism and quantitative variation of ?-amylases form rye endosperm

100%
Masojc P., Lapinski M., Larsson-Raznikiewicz M.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 3
197-204
9

Genetic diversity of inbred rye lines evaluated by RAPD analysis

88%
Myskow B., Masojc P., Banek-Tabor A., Szolkowski A.
Journal of Applied Genetics
|
2001
|
vol. 42
|
issue 1
1-14
EN
This study presents an attempt to supply breeders of hybrid rye with more genetic information on inbred lines, using molecular markers. Eighteen polymorphic loci detected by means of the RAPD (Random Amplified Polymorphic DNA) technique and mapped on 2R-7R rye chromosomes, were applied to study genetic similarities among forty inbred lines of rye. The lines were grouped in four main clusters revealed on dendrogram, which was generally consistent with the pedigree data. Mapped RAPD markers were shown to be a useful tool for phenetic studies in rye. Additionally, a system of 20 polymorphic fragments, detected by three primers, was developed for fingerprinting of rye lines. The system of RAPD markers, which was developed in this study, should be helpful in characterisation of rye genetic stocks used for breeding.
10

Production and morphology of the hybrids Aegilops kotschyi ? Secale cereale and Ae. biuncialis ? S. cereale

88%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 3
279-285
EN
Hybrids between Aegilops kotschyi and Ae. biuncialis with Secale cereale were synthesized. Five Ae. kotschyi and four Ae. biuncialis accessions, as well as one inbred and four self-compatible forms of Secale cereale were used for crossing. The hybrids were produced directly from cultured embryos or through embryo callus culture. Sixty hybrids, 11 involving Ae. kotschyi and 49 Ae. biuncialis, had a stable somatic chromosome number 2n = 3x = 21. The plants showed good vegetative vigour and tillering capacity. Morphologically the hybrids were intermediate between their parents and completely sterile. In vitro propagation of Ae. kotschyi and Ae. biuncialis ? S. cereale hybrids revealed that their capacity for callus production and plantlet regeneration ? varies.
11

Hybrids and amphiploids of Aegilops ovata L. with Secale cereale L.: production, morphology and fertility

88%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2002
|
vol. 43
|
issue 4
415-421
EN
Hybrids (2n = 3x = 21) between Aegilops ovata and Secale cereale were produced via embryo rescue. Most hybrid morphological traits were intremediate between parents, the plants grew vigorously but were completely sterile. The average frequency of chromosome associations at metaphase I was 19.57-20.19 univalents and 0.40-0.86 rod bivalents. The fertility of the hybrids was restored by doubling their chromosome numbers by colchicine treatment and in vitro propagation. Selfed seeds were obtained from colchicine-doubled sectors and some callus regenerates. The seeds were mostly well formed and developed vigorous plants without embryo culture. Colchicine- and callus-derived amphiploids (2n = 6x = 42) resembled the F1 plants in overall morphology, but showed a lower tillering ability, broader leaves, thicker culms and larger spikes.
12

Cytogenetic effects of Fusarium mycotoxins on root tip cells of rye (Secale cereale L.), wheat (Triticum aestivum L.) and field bean (Vicia faba L. var. minor)

88%
Packa D.
|
|
vol. 38
|
issue 3
259-272
EN
The effects of fusarial toxins: DAS, T 2, DON, 3 Ac DON, MON and ZEA, on actively dividing root tip cells of rye, wheat and field bean were investigated. Three concentrations: 1, 5 and 10 mg mL-1 were applied for 24 hours. Nuclei and chromosomes were stained using the Feulgens method. It has been found that trichothecene mycotoxins (DAS, T 2, DON, 3 Ac DON) had a profound effect on mitosis, as they decreased the mitotic index, produced excessive condensation of pro and metaphase chromosomes, C metaphases and C anaphases, and caused an accumulation of metaphases. The main effect of trichothecene mycotoxins, probably caused via their influence on protein synthesis, was abnormal functioning of the mitotic spindle.
13

Genetic regulation of alpha-amylase synthesis in rye (Secale cereale L.) grain

88%
Masojc P., Stojalowski S., Lapinski M., Miazga D.
Journal of Applied Genetics
|
1996
|
vol. 37
|
issue 2
141-152
EN
Genetic analysis of two rye interline crosses and a set of wheat/rye chromosomal additoin lines was performed to reveal the mechanism underlying wide variation range of alpha-amylase activity in sound grain.THe long arm of chromosome 6R was found to be responsible for increased enzyme synthesiis during late stages of triticale grain maturation.Only nuclear genes seemed to control alpha-amylase activity, as reciprocal crosses between rye lines showed no maternal effects.Low enzyme activity showed complete dominance over high level of its synthesis.Segregation ratios, observed in F2 and BC1 crosses, indicated that recessive alleles at two independent duplicative loci underline intensive alpha-amylase production.
14

Molecular cytogenetic evaluation of chromosome instability in Triticum aestivum?Secale cereale disomic addition lines

88%
Szakacs E., Molnar-Lang M.
|
|
vol. 51
|
issue 2
149-152
EN
The genetic stability of wheat/rye ('Chinese Spring'/'Imperial') disomic addition lines was checked using the Feulgen method and fluorescent in situ hybridization (FISH). Feulgen staining detected varying proportions of disomic, monosomic, and telosomic plants among the progenies of the disomic addition lines. The greatest stability was observed for the 7R addition line, while the most unstable lines were those with 2R and 4R additions. Chromosome rearrangements were also detected using FISH. Based on the specific hybridization patterns of repetitive DNA probes pSc119.2 and (AAC)5, as well as ribosomal DNA probes (5S and 45S), isochromosomes were identified in the progenies of 1R and 4R addition lines. The results draw attention to the importance of continuous cytological checks on basic genetic materials by using FISH, because this method reveals chromosome rearrangements that could not be detected either with the conventional Feulgen staining technique or with molecular markers.
15

Characterization of omega-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line

75%
|
|
vol. 51
|
issue 4
403-411
EN
Sixty-two DNA sequences for the coding regions of omega-secalin (-secalin) genes have been characterized from rye (Secale cereale L.), hexaploid and octoploid triticale (x Triticosecale Wittmack), and wheat (Triticum aestivum L.) 1BL/1RS translocation line. Only 19 out of the 62 omega-secalin gene sequences were full-length open reading frames (ORFs), which can be expressed into functional proteins. The other 43 DNA sequences were pseudogenes, as their ORFs were interrupted by one or a few stop codons or frameshift mutations. The 19 -secalin genes have a typical primary structure, which is different from wheat gliadins. There was no cysteine residue in -secalin proteins, and the potential celiac disease (CD) toxic epitope (PQQP) was identified to appear frequently in the repetitive domains. The -secalin genes from various cereal species shared high homology in their gene sequences. The omega-secalin gene family has involved fewer variations after the integration of the rye R chromosome or whole genome into the wheat or triticale genome. The higher Ka/Ks ratio (i.e. non-synonymous to synonymous substitutions per site) in omega-secalin pseudogenes than in -secalin ORFs indicate that the pseudogenes may be subject to a reduced selection pressure. Based on the conserved sequences of omega-secalin genes, it will be possible to manipulate the expression of this gene family in rye, triticale, or wheat 1BL/1RS translocation lines, to reduce its negative effects on grain quality.
16

The inheritance of plant height in crosses involving short-stem rye genotypes

75%
Izdebski R.
Journal of Applied Genetics
|
1995
|
vol. 36
|
issue 4
325-340
EN
The paper presents results of studies on the inheritance of plant height in four short-stem genotypes of rye selected from genotypically different collection materials.Short-stem genotypes P-2, P-9, P-30 and P-52 were crossed to a tall-stem inbred line Uniwersalne 145 (S17).The genotype P-2 was additionally crossed to a related, but tall-stem line P-2 W.In each cross combnations six generation -P1, P2, F1, F2, B1 (F1 x P10 and B2 (F1 x P2) - were examinaed.Distribution of plant height in hybrid generations haad a continous character, which indicated a polyugenic determination of short stem an all "P" genotypes under study.Results of the genetic analysis showed a significant role of additive, dominance and epistatic gene action in genetic determinatin of short stem in th "P" genotypes.
17

Isozymes in Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids and Ae. kotschyi ? S. cereale amphiploids in relation to their parents

75%
Kalinowski A., Wojciechowska B., Borzyszkowska E.
Journal of Applied Genetics
|
2003
|
vol. 44
|
issue 1
35-43
EN
Seven enzymatic systems in F1 Aegilops kotschyi and Ae. biuncialis ? Secale cereale hybrids, Aegilops kotschyi ? S. cereale amphiploids and their parental species (Ae. kotschyi, Ae. biuncialis and S. cereale) were analysed by starch and polyacrylamide gel electrophoresis. Five of them (phosphoglucose isomerase, glutamic oxalacetic transaminase, esterase, acid phosphatase, and diaphorase) were polymorphic and two (malic dehydrogenase and superoxide dismutase) were monomorphic. Several isophorms of phosphoclucose isomerase, esterase, acid phosphatase, and diaphorase were detected in some hybrids and amphiploids, but absent in the parents. The role of regulators, translocations and recombination is discussed in relation to the origin of these new isophorms. Some parental isozymes were absent both in hybrids and amphiploids, probably as a result of the suppression of structural genes in new combinations of the three genomes.
18

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis ? S. cereale amphiploids with 2x and 4x S. cereale

75%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2005
|
vol. 46
|
issue 2
157-161
EN
Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F1 sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F1 plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis ? S. cereale amphiploid) ? S. cereale hybrids via embryo culture. The BC1 plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC1 hybrids.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.