PSJD - Yadda

1

Identification and characterization of high-molecular-weight glutenin genes in Polish triticale cultivars by PCR-based DNA markers

100%

Salmanowicz B. P., Dylewicz M.

Journal of Applied Genetics

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2007

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vol. 48

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issue 4

347-357

EN

Molecular markers were used to identify the allele/gene composition of complex loci Glu-A1 and Glu-B1 of high-molecular-weight (HMW) glutenin subunits in triticale cultivars. Forty-six Polish cultivars of both winter and spring triticale were analysed with 7 PCR-based markers. Amplified DNA fragments of HMW glutenin Glu-1 genes were separated by agarose slab-gel electrophoresis. Differences between all 3 alleles at the locus Glu-A1 [Glu-A1a (encoding Ax1), 1b (Ax2*), and 1c (AxNull)], 4 alleles at Glu-B1-1 [Glu-B1-1a (Bx7), 1b (Bx7*), 1d (Bx6), 1ac (Bx6.8)], and 5 alleles at Glu-B1-2 [Glu-B1-2a (By8), 2b (By9), 2o (By8*), 2s (By18*), and 2z (By20*)] were revealed. In total, 16 allele combinations were observed. Molecular markers are particularly helpful in distinguishing the wheat Glu-A1a and Glu-A1b alleles from the rye Glu-R1a and Glu-R1b alleles in triticale genotypes, respectively, as well as subunits Bx7 from Bx7* and By8 from By8*, which could not be distinguished by SDS-PAGE. Novel glutenin subunits By18* and By20* (unique to triticale) were identified. HMW glutenin subunit combinations of Polish triticale cultivars, earlier identified by SDS-PAGE analyses, were verified by PCR-based DNA markers. Rapid identification of wheat Glu-1 alleles by molecular markers can be an efficient alternative to the standard separation procedure for early selection of useful triticale genotypes with good bread-making quality.

2

Cucumber: A model angiosperm for mitochondrial transformation?

100%

Havey M. J., Lilly J. W., Bohanec B., Bartoszewski G., Malepszy S.

Journal of Applied Genetics

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2002

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vol. 43

|

issue 1

1-17

EN

Plants possess three major genomes, carried in the chloroplast, mitochondrion, and nucleus. The chloroplast genomes of higher plants tend to be of similar sizes and structure. In contrast both the nuclear and mitochondrial genomes show great size differences, even among closely related species. The largest plant mitochondrial genomes exist in the genus Cucumis at 1500 to 2300 kilobases, over 100 times the sizes of the yeast or human mitochondrial genomes. Biochemical and molecular analyses have established that the huge Cucumis mitochondrial genomes are due to extensive duplication of short repetitive DNA motifs. The organellar genomes of almost all organisms are maternally transmitted and few methods exist to manipulate these important genomes. Although chloroplast transformation has been achieved, no routine method exists to transform the mitochondrial genome of higher plants. A mitochondrial-transformation system for a higher plant would allow geneticists to use reverse genetics to study mitochondrial gene expression and to establish the efficacy of engineered mitochondrial genes for the genetic improvement of the mitochondrial genome. Cucumber possesses three unique attributes that make it a potential model system for mitochondrial transformation of a higher plant. Firstly, its mitochondria show paternal transmission. Secondly, microspores possess relatively few, huge mitochondria. Finally, there exists in cucumber unique mitochondrial mutations conditioning strongly mosaic (msc) phenotypes. The msc phenotypes appear after regeneration of plants from cell culture and sort with specific rearranged and deleted regions in the mitochondrial genome. These mitochondrial deletions may be a useful genetic tool to develop selectable markers for mitochondrial transformation of higher plants.

3

The utilization of molecular biology methods in farm animals breeding and selection

100%

Slota E., Rejduch B., Radko A.

Biotechnologia

|

2003

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issue 1

84-92

EN

On the basis of the literature, the new molecular methods useful in animal breeding and selection are described. DNA restriction fragment lenght polymorphism analysis is a tool in the diagnosis of some genetic diseases (RYR1 in pigs, BLAD and DUMPS in cattle). Microsatellite DNA polymorphism is useful in parentage control, genetic characteristic of populations, as well as in gene mapping and marker assisted selection. Cytogenetic analyses are recently supported by fluorescent in situ hybridization (FISH) and primed in situ labelling (PRINS) which make the chromosome aberration diagnosis more precise. One of the expanded method is bio-chip construction for genome analyses.

4

Mycobacteria in current genetic research

100%

Golanska E., Sasiak A., Jaworski A., Chmiel A.

Biotechnologia

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1998

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issue 3

72-88

EN

The article reviews the development of molecular investigations of the genus Mycobacterium. Achievements and difficulties of the genetics of mycobacteria are presented. Vectors and selectable markers commonly used in genetic manipulations and methods of introduction of foreign DNA into mycobacteria are described. The stress is laid not only on the research of the virulent mycobacteria, but also on molecular investigations of the non-pathogenic strains used in steroid drugs industry.

5

Routine clinical laboratory tests correspond to increased serum levels of 3-hydroxy fatty acids, markers of endotoxins, in cardiosurgery patients

100%

Krasnik L., Szponar B., Walczak M., Larsson L., Gamian A.

Archivum Immunologiae et Therapiae Experimentalis

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2006

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vol. 54

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issue 1

55-60

EN

Introduction: Endotoxemia developing during cardiosurgery as elevated endotoxin concentrations in patient's serum may prevail over 24 h after operation. A major reason is thought to be increased gut permeability resulting in endotoxin and bacterial leakage. In this study we aimed to measure endotoxin levels on samples obtained during and after cardiovascular procedures and compare them with clinical observations and laboratory test results. Materials and Methods: 3-Hydroxy fatty acids (3-OH FAs) of 10?18 carbon chain length, chemical markers of endotoxin (lipopolysaccharide), were determined in patient sera by gas chromatography-mass spectrometry-based analysis. Results were compared with routine laboratory tests: blood morphology, urine, ALT, AST, bilirubin, kidney parameters, clotting parameters, and gasometry. Results: Of a total of 16 patients, 5 patients (group I) showed increased serum 3-OH FA levels and 11 patients (group II) did not show any change in 3-OH FA levels 24 h after operation. All group I patients revealed leukocytosis, two developed post-operative anemia. Significantly different changes were observed: the initial, pre-operative 3-OH FA levels were similar for both groups, while group I patients showed increased levels of all the studied 3-OH FAs during the operation (p0.05), and 3-OH C14 and 3-OH C16 remained elevated 24 h after the operation. Conclusions: Cardiosurgery may strongly promote gut endotoxin translocation to the blood in some patients. Prolonged leukocytosis, deep anemia, and increased liver dysfunction markers may indicate the need for observation of possible endotoxemia development. It is recommended to monitor the endotoxin level and/or endotoxemia markers in cardiosurgery patients.

6

Usefulness of RAPD for genetic distance estimation in the European species Pinus sylvestris

100%

Szyp-Borowska I., Staniulyte R.

Biotechnologia

|

2003

|

issue 2

280-289

EN

Random Amplified Polymorphic DNAs (RAPD) technique with single primers was tested for its usefulness in genetic distance estimation and population studies in the coniferous species Pinus sylvestris. DNA markers allow for direct analysis both coding and noncoding regions of the genome. The technique of detection DNA variations using RAPD markers has become a popular tool in genetic studies. Different reaction conditions were tested in order to get the optimal resolution of fragments, specificity and reproducibility of patterns. In this preliminary investigation, a high MgCl2 concentration (5,5 mM) together with a low primer concentration (0,2 M) in the polymerase chain reaction (PCR) mixture yielded the best amplification products. Amplified fragments were scored as the presence or absence fragments.

7

Resistance of spring wheat cultivars and lines to leaf rust

100%

Wisniewska H., Stepien L., Kowalczyk K.

Journal of Applied Genetics

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2003

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vol. 44

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issue 3

361-368

EN

Spring wheat nursery accessions, including 18 spring wheat lines derived in CIMMYT, Mexico, and 12 spring wheat cultivars bred in Poland, along with cultivars Frontana and Sumai 3 as resistant controls, were examined for resistance to leaf rust under field conditions. Multipathotype tests with 16 different pathogen isolates were performed for postulation of Lr genes in Polish cultivars. Besides, STS markers for resistance genes Lr1, Lr9, Lr10, Lr24, Lr28, Lr37 were analysed in the studied cultivars and lines with Thatcher near-isogenic lines as positive controls. All Polish cultivars appeared to be susceptible to leaf rust. Ten of the CIMMYT nursery lines (IPG-SW: #7, 11, 14, 21, 22, 23, 27, 29, 30, 32) and cv. Frontana were resistant in the same environment and can be sources of resistance genes. Marker for the Lr10 gene was identified in 6 accessions (IPG-SW #14, 22, 23, 29, 30, 32) exhibiting resistance to leaf rust, whereas markers for Lr1 and Lr28 genes were observed in all the examined accessions. STS markers for Lr9, Lr24 and Lr37 genes were not identified in the investigated accessions.

8

Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers

100%

Moczulski M., Salmanowicz B. P.

Journal of Applied Genetics

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2003

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vol. 44

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issue 4

459-471

EN

Wheat bread-making quality is closely correlated with composition and quantity of gluten proteins, in particular with high-molecular weight (HMW) glutenin subunits encoded by the Glu-1 genes. A multiplex polymerase chain reaction (PCR) method was developed to identify the allele composition of HMW glutenin complex Glu-1 loci (Glu-A1, Glu-B1 and Glu-D1) in common wheat genotypes. The study of multiplex PCR to obtain a well-balanced set of amplicons involved examination of various combinations of selected primer sets and/or thermal cycling conditions. One to three simultaneously amplified DNA fragments of HMW glutenin Glu-1 genes were separated by agarose slab-gel electrophoresis and differences between Ax1, Ax2* and Axnull genes of Glu-A1 loci, Bx6, Bx7 and Bx17 of Glu-B1, and Dx2, Dx5 and Dy10 genes of Glu-D1 loci were revealed. A complete agreement was found in identification of HMW glutenin subunits by both multiplex PCR analysis and SDS-PAGE for seventy-six Polish cultivars/strains of both spring and winter common wheat. Rapid identification of molecular markers of Glu-1 alleles by multiplex PCR can be an efficient alternative to the standard separation procedure for early selection of useful wheat genotypes with good bread-making quality.

9

Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes

100%

Su H., Wang L., Liu L., Chi X., Zhang X.

Journal of Applied Genetics

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2008

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vol. 49

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issue 3

233-235

EN

In this paper, we report for the first time on authentication of Flammulina velutipes cultivars by using strain-specific sequence-characterized amplified region (SCAR) markers developed from inter-simple sequence repeat (ISSR) markers. The genomic DNA polymorphism was analyzed by the ISSR technique in 7 strains of F. velutipes presently cultivated in China on a commercial scale. Eight primers selected from 20 ISSR primers amplified 104 clear and stable bands, of which 81 bands were polymorphic. Among the selected primers, primer ISSR9 can distinguish strain No. 12 from the other 6 strains by amplifying a unique and reproducible band of approximately 750 bp. According to the sequence of the strain-specific fragment, a pair of SCAR primers was designed to diagnose strain No. 12 on the molecular level. The validity of the SCAR marker was confirmed by using DNA samples from another 12 strains of F. velutipes obtained from different parts of China. Our data provided the foundation for a precise and rapid PCR-based strain-diagnostic system for F. Velutipes.

10

Cucumber transformation methods - the review

100%

Yin Z., Bartoszewski G., Szwacka M., Malepszy S.

Biotechnologia

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2005

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issue 1

95-113

EN

Several aspects of cucumber transformation including the ways of transgene introduction, factors influencing the transformation efficiency and the fate of the introduced genes were reviewed. Various transgenes have been introduced into the cucumber genome mostly via the Agrobacterium-mediated transformation. The frequency of Agrobacterium-mediated transformation ranged from 0.8 to 10% and was influenced by the selection agent, the regeneration efficiency, activation of vir genes expression, the explant size, bacteria cell density, the length of exposure and the co-cultivation period. The transgenes were integrated mostly as single copy in the Agrobacterium-mediated transformation and as multiple copies in direct transformation. Variable levels of the transgene expression were observed. The transmission of the transgenes as well as the transgenic phenotype follow the Mendelian, and rarely non-Mendelian, ratio. The production of marker-free transgenic cucumber and use of an alternative transformation method are recommended.

11

Random amplified polymorphic DNA fingerprinting as a marker for Paramecium jenningsi strains

100%

Skotarczak B., Przybos E., Wodecka B., Maciejewska A.

Folia Biologica

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2004

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vol. 52

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issue 1-2

117-124

EN

The aim of the present study is to establish a common RAPD marker for P. jenningsi using a series of Ro primers and to investigate if strains originating from distant and isolated localities (Japan, China, India, Saudi Arabia) have isolated gene pools and represent distinct species. An analysis of dendrograms constructed on the basis of RAPD-PCR fingerprints with four primers (Ro 460-04, 460-06, 460-07, and 460-10) from the first part of this project (SKOTARCZAK et al. 2004), assigns the strains to two groups consisting of the continental strains (India, Saudi Arabia, China) and Japanese strains that have been considered as a separate sibling species within P. jenningsi. The genetic similarity of the Indian and Arabian strains was ascertained, whereas the Chinese strain formed an independent branch in this sibling species. The primers Ro (460-01, 460-02, 460-03, 460-05, 460-08) also distinguish between two groups of strains, although they divide the Japanese strains into two subgroups that are not reproductively isolated. This probably indicates genetic variation within this sibling species. However, it comprises one common gene pool (successful inter-strain crosses) and is reproductively isolated from the other sibling species. The results presented in these papers confirm that the construction of ten band patterns having marker attributes is possible on the basis ofDNAamplification from 9 strains of P. jenningsi with the RAPD-PCR fingerprinting method using five primers from the Ro series. The patterns can be assigned to three marker-groups: a general species group, a group differentiating between sibling species, and accessory strain markers.

12

Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis

100%

Ngezahayo F., Dong Y., Liu B.

Journal of Applied Genetics

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2007

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vol. 48

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issue 4

329-336

EN

The nature of somaclonal variation at the nucleotide sequence level was studied in rice cv. Nipponbare. First, we investigated genomic variations by using 2 molecular marker systems: RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This was followed by sequencing of selected bands that represented genomic variations, and pairwise sequence analysis taking advantage of the whole genome sequence of rice. In addition, transpositional activity of the active MITE, mPing, was analysed by locus-specific PCR amplifications. The 2-year-old calli and their regenerated plants, analysed with 24 RAPD and 20 ISSR primers, showed moderate levels of genomic variation (20.83% and 17.04%, respectively). To test whether DNA methylation plays a role in somaclonal variation, the calli were treated with 5-azacytidine, a chemical agent that reduces cytosine methylation by blocking the activity of DNA methyltransferase. Though dwarfism occurred in regenerants from treated calli (a hallmark of the drug treatment), there was only a slight increase in the frequency of somaclonal variation detected in the treated calli and their regenerated plants relative to untreated controls. The transposon mPing also remained immobile in both treated and untreated calli. Nevertheless, dendrograms constructed according to the Jaccard coefficient calculated by UPGMA of the ISSR bands revealed that the 5-azacytidine-treated and untreated somaclones were grouped into 2 distinct clusters, suggesting a possible indirect effect of the treatment on the genomic changes, depending on the marker used. Sequence analysis indicated a low level of variation (0.31%), with single-base-pair substitutions predominating.

13

Methods of measurement of the protein glycation products formed in human organism

100%

Staniszewska M., Gamian A.

Postępy Higieny i Medycyny Doświadczalnej

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2003

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vol. 57

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issue 4

359-368

EN

The advanced glycation end-products (AGEs) are the compounds formed and accumulated in the organism. There is heterogenous group of the glycation products, which have great variety of structural and functional properties. The glycation end-products can be used as markers of the glycation process. Because of the great heterogeneity of the AGEs there is no specific test for their adequate measurement. In this paper there are presented the chromatographic, colorimetric, spectroscopic, mass-spectrometric and serological methods, which are used for determination of AGEs in biological samples. New procedures for the preparation of the model AGEs have been also described. Special attention has been paid to the immunochemical tests. For further assays the model AGE antigens have to be prepared.

14

Introgression of Brassica napus genome into restorer lines for CMS ogura estimated with the use of molecular markers

100%

Poplawska W., Bartkowiak-Broda I.

Biotechnologia

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2001

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issue 1

112-118

EN

The object of research were double low restorer lines of CMS ogura. The restorer gene was introgressed into rapeseed genotype from radish (Raphanus sativus) through intergeneric hybridisation. The obtained recombinants of rapeseed with the restorer gene comprise a DNA fragment originating from radish, which is bigger than the locus of the restorer gene. It disturbs the first meiotic behaviour in PMC. In addition, it has an impact on the fertility and yielding ability of restorer lines. Moreover, the restorer gene in the obtained recombinant is tightly linked with a gene responsible for high content of undesirabled compounds in seeds - glucosinolates. Elimination of the unnecessary DNA fragment is performed by backcrosses with double low lines of winter rapeseed. The changes in rapeseed genome which are a result of backcrosses are investigated by the use of molecular and isozyme markers. Key words: Brassica napus, restorer gene, introgression, molecular and isozyme markers. Adres do korespondencji:

15

Influence of dent corn genetic backgrounds on QTL detection for plant-height traits and their relationships in high-oil maize

100%

Wei M., Fu J., Li X., Wang Y., Li Y.

Journal of Applied Genetics

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2009

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vol. 50

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issue 3

225-234

EN

QTL mapping for plant-height traits has not been hitherto reported in high-oil maize. A high-oil maize inbred 'GY220' was crossed with two dent maize inbreds ('8984' and '8622') to generate two connected F2:3 populations. Four plant-height traits were evaluated in 284 and 265 F2:3 families. Single-trait QTL mapping and multiple-trait joint QTL mapping was used to detect QTLs for the traits and the genetic relationship between plant height (PH) and two other plant-height traits. A total of 28 QTLs and 12 pairs of digenic interactions among detected QTLs for four traits were detected in the two F2:3 families. Only one marker was shared between the two populations. Joint analysis of PH with ear height (EH) and PH with top height (TH) detected 32 additional QTLs. Our results showed that QTL detection for PH was dependent on the genetic background of dent corn inbreds. Multiple-trait joint QTL analysis could increase the number of detected QTLs.

16

Resistance genes in wild accessions of Triticeae ? inoculation test and STS marker analyses

100%

Stepien L., Holubec V., Chelkowski J.

Journal of Applied Genetics

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2002

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vol. 43

|

issue 4

423-435

EN

Sequence tagged site (STS) markers have been developed recently to identify resistance genes in wheat. A number of wild relatives have been used to transfer resistance genes into wheat cultivars. Accessions of wild species of Triticeae: Aegilops longissima (4), Ae. speltoides (6), Ae. tauschii (8), Ae. umbellulata (3), Ae. ventricosa (3), Triticum spelta (2), T. timopheevi (3), T. boeoticum (4) and T. monococcum (1), 34 in total, were examined using PCR-STS markers for resistance genes against Puccinia recondita f.sp. tritici (Lr) and Erysiphe graminis (Pm). Additionally, a set of cv. Thatcher near-isogenic lines conferring resistance genes Lr 1, Lr 9, Lr 10, Lr 24, Lr 28, Lr 35 and Lr 37 were examined with the same procedure. Twenty-two accessions were tested using the inoculation test for resistance to Erysiphe graminis, Puccinia recondita, P. striiformis and P. graminis. The most resistant entries were those of Aegilops speltoides and Triticum timopheevi and among T. boeoticum accession #5353. Markers of all mentioned Lr resistance genes were identified in all corresponding cv. Thatcher near-isogenic lines (except Lr 35 gene marker). The following resistance gene markers were identified in wild Triticeae accessions: Lr 1 in two accessions of Ae. tauschii and one accession of Ae. umbellulata, Lr 9 in one accession of Ae. umbellulata, Lr 10 in one accession of T. spelta, Lr 28 in 11 accessions: Ae. speltoides (4), Ae. umbellulata (2), T. spelta (2) and T. timopheevi (3), Lr 37 in 3 accessions of Ae. ventricosa, Pm 1 in all 34 accessions, Pm 2 in 28 accessions, Pm 3 in all 4 accessions of T. boeoticum, 1 accession of T. spelta and 1 of T. timopheevi, and Pm 13 in 5 out of 6 accessions of Ae. speltoides. Reliability and usefulness of STS markers is discussed.

17

Endothelial progenitor cells as a new agent contributing to vascular repair

100%

Miller-Kasprzak E., Jagodzinski P. P.

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2007

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vol. 55

|

issue 4

247-259

EN

A special type of stem cells, defined as endothelial progenitor cells (EPCs), has been found in the bone marrow and peripheral blood. These EPCs are incorporated into injured vessels and become mature endothelial cells during re-endothelialization and neovascularization processes. Though a complete phenotypic description of EPCs remains unclear, these cells express several surface markers, the most relevant including CD34 and CD133 antigens. Furthermore, EPCs derived from other sources could also give rise to mature endothelial cells, which makes this group of cells more diverse. The recruitment of EPCs from the bone marrow to homing sites of vasculogenesis is subject to regulation by many factors, including chemokines and growth factors. The precise mechanism of EPC mobilization and differentiation is not entirely elucidated and is still under investigation. Recent studies have suggested that EPCs may promote local angiogenesis by secreting angiogenic growth factors in a paracrine manner. The number and function of EPCs can be affected during pathological conditions, including diabetes mellitus, cardiovascular risk factors for ischemic disease, and graft vasculopathy. Additionally, EPC number and migration capacity could be improved by such factors as drugs, physical exercise, and growth factors. Transplantation of EPCs into ischemic tissues may emerge as a promising approach in the therapy of diseases associated with blood vessel disorders.

18

Turner syndrome mosaicism: an unusual case with a de novo large dicentric marker chromosome: mos 45,X/46,X, ter rea(X;X)(p22.3;p22.3)

100%

Nucaro A. L., Melis P., Casini M. R., Rossino R., Cau M., Melis M. A., Loche S.

Journal of Applied Genetics

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2008

|

vol. 49

|

issue 3

301-303

EN

X/X translocations are quite rare in humans. The effect of this anomaly on the phenotype is variable and depends on the amount of deleted material and whether the chromosomes are joined by their long or short arms. We report an unusual case of Turner syndrome mosaicism in a 16-year-old girl, who was referred to our Institute for primary amenorrhoea associated with short stature. Endocrine evaluation revealed hypergonadotropic hypogonadism, which required a study of the karyotype. Cytogenetic analysis, performed on peripheral blood leucocytes, showed a mos 45,X/46,X,ter rea (X;X)(p22.3;p22.3) de novo karyotype. The prevalent cell line was 45,X (90% cells). A second cell line (10% cells) showed a very large marker chromosome, similar to a large metacentric chromosome. FISH (fluorescent in situ hybridisation) and molecular analysis revealed that the marker chromosome was dicentric and totally derived from the paternal X chromosome.

19

Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

88%

Szajko K., Chrzanowska M., Strzelczyk-Zyta D., Zagorska H., Marczewski W.

Journal of Applied Genetics

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2008

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vol. 49

|

issue 1

45-47

EN

The Ns gene confers resistance of potato to Potato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of the Ns-linked SCAR marker SC811454 revealed additional amplicons with a similar electrophoretic migration rate as that of SC811454, which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect their Ns-unspecificity in PVS-susceptible potato cultivars.

20

Identification of RAPD markers in plants

88%

Marczewski W.

Biotechnologia

|

1999

|

issue 1

106-115

EN

The random amplified polymorphic DNA (RAPD) method is based on random amplification of DNA fragments, via PCR, using short primers of arbitrary sequence. RAPD markers have been applied to construct linkage maps, to assess genetic diversity, to study taxonomic relationships, and to tag disease resistance genes in plants. RAPD markers linked to a resistance gene can be identified using bulked segregant analysis (BSA), recombinant inbred lines (RILs) or near-isogenic lines (NILs). More reliable and specific PCR-based markers known as sequence-characterized amplified region (SCAR) and allele-specific associated primer (ASAP) were developed. There are several examples of the application of these DNA marker systems in marker-assisted plant breeding.

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Identification and characterization of high-molecular-weight glutenin genes in Polish triticale cultivars by PCR-based DNA markers

Cucumber: A model angiosperm for mitochondrial transformation?

The utilization of molecular biology methods in farm animals breeding and selection

Mycobacteria in current genetic research

Routine clinical laboratory tests correspond to increased serum levels of 3-hydroxy fatty acids, markers of endotoxins, in cardiosurgery patients

Usefulness of RAPD for genetic distance estimation in the European species Pinus sylvestris

Resistance of spring wheat cultivars and lines to leaf rust

Multiplex PCR identification of wheat HMW glutenin subunit genes by allele-specific markers

Use of inter-simple sequence repeat markers to develop strain-specific SCAR markers for Flammulina velutipes

Cucumber transformation methods - the review

Random amplified polymorphic DNA fingerprinting as a marker for Paramecium jenningsi strains

Somaclonal variation at the nucleotide sequence level in rice (Oryza sativa L.) as revealed by RAPD and ISSR markers, and by pairwise sequence analysis

Methods of measurement of the protein glycation products formed in human organism

Introgression of Brassica napus genome into restorer lines for CMS ogura estimated with the use of molecular markers

Influence of dent corn genetic backgrounds on QTL detection for plant-height traits and their relationships in high-oil maize

Resistance genes in wild accessions of Triticeae ? inoculation test and STS marker analyses

Endothelial progenitor cells as a new agent contributing to vascular repair

Turner syndrome mosaicism: an unusual case with a de novo large dicentric marker chromosome: mos 45,X/46,X, ter rea(X;X)(p22.3;p22.3)

Endonuclease restriction of SCAR amplicons SC811 is required to identify Ns-false-positive markers in PVS-susceptible potato cultivars

Identification of RAPD markers in plants