Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl
Preferences help
Polish version English version Language
enabled [disable] Abstract
Number of results

Results found: 12

Number of results on page
first rewind previous Page / 1 next fast forward last

Search results

Search:
in the keywords:  HAIRY ROOT
help Sort By:

help Limit search:
first rewind previous Page / 1 next fast forward last
1

Formation and characterization of hairy roots

100%
Wasilewska A., Krolicka A.
Biotechnologia
|
2005
|
issue 4
173-188
EN
Agrobacterium rhizogenes is a soil phytopathogen, which infects wounded plant tissue and generates overproliferation of neoplastic roots. Hairy-root cultures obtained by transformation of plant tissue by A. rhizogenes have evolved as an important tool for biosynthesis of plant secondary metabolites. This review discusses the methods for efficient plant transformation and hairy root formation for secondary metabolites production.
2

Endogenous hydrolysis of glucotropaeolin to benzyl isothiocyanate in hairy root cultures of Tropaeolum majus L.

80%
Wielanek M., Urbanek H., Majorowicz H.
Biotechnologia
|
2004
|
issue 1
210-220
EN
The conditions of endogenous hydrolysis of glucotropaeolin to bioactive benzyl isothiocyanate for T. majus hairy root cultures giving high yields of glucotropaeolin and myrosinase were optimized. After in vitro glucotropaeolin hydrolysis at pH 5.5, optimal for myrosinase activity, 54% of glucotropaeolin were converted to benzyl isothiocyanate, 22% to benzyl cyanide, and 1.9% to benzyl thiocyanate. During endogenous glucotropaeolin hydrolysis, maximal benzyl isothiocyanate yield, 87% of tissue glucotropaeolin, was detected at pH 7.5, in the 60th min of incubation. In the presence of 100 M ascorbic acid, the rate of hydrolysis increased and in the 60th min 99% of glucotropaeolin was converted to benzyl isothiocyanate. The highest benzyl isothiocyanate yield, 0.746 mmol/10 g fresh weight, was obtained during endogenous hydrolysis at pH 7.5, in the presence of 100 M ascorbic acid from the hairy roots with glucotropaeolin content, enhanced by precursors (phenylalanine + cystein) and inhibitor of phenylalanine ammonia-lyase activity (l-1-amino-2-phenylethylphosphonic acid).
3

Resistance to antibiotics of the selected species of Agrobacterium rhizogenes

80%
Krolicka A., Kurlenda J., Lojkowska E.
Biotechnologia
|
1999
|
issue 2
94-103
EN
Agrobacterium rhizogenes is used for the transformation of plant cells and production of hairy roots cultures. In the presented work the bacteriostatic activity of several antibiotics on A. rhizogenes strains was tested. Different concentration of antibiotics belonging to the types of cefalosporin II and III generation, b-lactam and fluorochinolon were tested for elimination of the bacteria from transformed tissue. Out of all tested combinations, the mixture of carbenicilin and cefotaksym (claforan) was the most efficient for A. rhizogenes strains elimination from transformed plant tissues. The addition of those antibiotics to the regeneration medium was not toxic to plant tissues and it facilitated rapid growth of hairy roots.
4

Secondary metabolites from hairy roots of Tanacetum parthenium

80%
Stojakowska A., Kisiel W.
Biotechnologia
|
2003
|
issue 3
87-94
EN
Feverfew (Tanacetum parthenium (L.) Sch. Bip.) is an ornamental and medicinal plant. Its preparations are mainly used for migraine prophylaxis. Complex mixture of T. parthenium secondary metabolites contain among others, sesquiterpene lactones and spiroketalenolether diacetylenes. From the roots of plant transformed with Agrobacterium rhizogenes LBA 9402 four diacetylenes and two coumarins were isolated, including the sesquiterpene coumarin ether 9-epipectachol B, a new natural product. The influence of exogenously applied salicylate and methyl jasmonate on diacetylene accumulation pattern was studied. Both signal molecules induced significant changes in the diacetylene accumulation suggesting that the compounds are involved in the plant defence.
5

Scaling up the Paulownia tomentosa hairy roots? culture

80%
Pawlowska B., Chmiel A.
Biotechnologia
|
2003
|
issue 4
228-238
EN
Hairy roots, characterized by rapid and stable growth, can be considered as a source of natural plant?s metabolites. The commercial utilization of roots is limited because of the difficulties during the culture in scaling up and designing of bioreactors, which could meet the requirements of optimal conditions of culture. The transformed root cultures of Paulownia tomentosa were conducted in shaking flasks of 300 cm3 containing 80 cm3 hormone-free WP medium, with 3% of sucrose. A mist bioreactor was also designed for transformed roots? cultures. Its glass prototype of 5 dm3 volume was used for optimization of hairy roots of Paulownia tomentosa. In the bioreactor containing 1 dm3 of the medium, after 28 days the dry mass harvest was 10.5-20.5 g depending on culture variants. The total amount of the main metabolite ? verbascoside in these roots was 8601170 mg in one culture (5672 mg/g of dry mass). The research performed on the prototype bioreactor enabled the optimization of its construction. It resulted in a new laboratory bioreactor of 10 dm3 volume made of glass and stainless steel.
6

Transformed roots as a source of secondary metabolites

80%
Olszowska O.
Biotechnologia
|
2000
|
issue 2
54-63
EN
In the recent years a lot of attention has been paid to hairy root culture as a promising strategy to produce a variety of secondary metabolites including pharmaceuticals, pigments and also fragrances. Hairy roots are tumorous outgrowth developing at the site of infection of wounded plant parts with gram-negative soil bacteria Agrobacterium rhizogenes. During the infection process a part of root-inducing (Ri) plasmid from bacteria is transferred to the plant cell where it integrates into the genome. An increasing number of hairy root cultures is known to produce secondary metabolites in quantities comparable with those in intact plant roots. Some secondary metabolites occurring only in the aerial parts of the intact plants were also found in the hairy roots of some species. So far, production of secondary metabolites in hairy root cultures of about 100 dicotyledonous species and some gymnosperms has been reported. The suitability of hairy root cultures for bioprocessing can be attributed to their genetic and biochemical stability and rapid growth in hormone-free media. Hairy root cultures of medicinal plants and factors influencing their productivity will be reviewed.
7

Hairy root culture of Centaurium erythraea Rafn.

80%
Piatczak E., Wielanek M., Krolicka A., Lojkowska E., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
29-36
EN
In this study, the transformed root culture of Centaurium erythraea was established, as a result of infection of leaves culture with agropine strain of Agrobacterium rhizogenes (LBA 9402). Frequency of root formation depended on the kind of explants and the presence of acetosyringone in the bacteria medium. The transformation was confirmed according to the analysis of opines and RAPD-PCR. As the result of transformation 30 clones of hairy roots were obtained. These clones have grown in liquid Woody Plant (WPM) medium without growth regulators. The curve of growth indicates that fresh weight of roots increased almost 17 times , and dry weight increased 12 times during 55 days. Hairy roots of C. erythraea were able to produce secoiridoid glycosides (sweroside, gentiopicroside, swertiamarine).
8

The conditions of transformed roots growth in bioreactors

70%
Pawlowska B., Ledakowicz S., Chmiel A.
Biotechnologia
|
2005
|
issue 2
88-102
EN
Transformed roots are also called transgenic roots or due to their appearance ? hairy roots. Transformed roots are obtained by infection of plants or parts of them with soil bacteria, Agrobacterium rhizogenes. Genetically transformed roots are characterised by rapid and stable growth of biomass, genetic stability, and stable biosynthesis of metabolites. Hairy roots can be an alternative source of many economically important chemicals like enzymes, pigments, flavours and especially bioactive pharmaceuticals. Cultures of transformed roots are comparatively easy to cultivate in shaking flasks. Application of flasks does not allow to obtain great amount of biomass. There is a necessity of scaling up the culture using bioreactors. The first attempts of scaling up the culture were connected with bioreactors designed for microbiological processes. However, these bioreactors are inadequate for hairy roots cultures. Some promising results were achieved in the case of using mist bioreactor. This paper is a review of bioreactors employed in cultures of hairy roots and mathematical modelling of hairy root growth.
9

Genetic modification of secondary metabolic pathways

70%
Wysokinska H.
Biotechnologia
|
2000
|
issue 4
48-61
EN
The examples given in this presentation show that the levels of secondary metabolites can be modified by genetic engineering methods. In these methods, the specific genes encoding enzymes involved in key steps of biosynthetic pathway can be transferred and expressed in cell lines, hairy root cultures or transgenic plants of the same or another species. Overexpression of the enzymes may lead to the accumulation of intermediates of pathways and increased synthesis of desirable products. Best results will be obtained when the overproduced enzyme activity is clearly the rate-limiting step in the pathway. Therefore, better knowledge of the limiting steps of the pathways is required. The successful of biosynthetic pathways regulation may also be achieved by expressing antisense RNA which is complementary to the mRNA encoding pathway enzyme or by genetic manipulation of degradative pathways. It should be mentioned that genetic engineering also plays a role in the development of novel plant-derived drugs. As secondary metabolites are important in the defense of plants against pathogens, such engineered plants may show an increase in resistance against pathogens.
10

Transformed root cultures of Arnica montana L. ? of secondary metabolites isolation

70%
Weremczuk-Jezyna I., Wysokinska H.
Biotechnologia
|
2004
|
issue 2
54-61
EN
The hairy roots of A. montana were achieved by the infection of sterile leaves with Agrobacterium rhizogenes (strain LBA 9402). The transformation was confirmed by opin and PCR analysis. In the investigation, the optimum contitions of Hairy roots growth were characterized. The best results were achieved in liquid Gamborg medium which included half-strenght macro- and microelements (1/2B5) and 50 g/l sucrose. Phytochemical analysis showed that hairy roots of A. montana produced arnifolin and chlorogenic acid.
11

Induction of glucotropaeolin biosynthesis and myrosinase activity in hairy roots of nasturtium

61%
Wielanek M., Urbanek H.
Biotechnologia
|
2000
|
issue 4
123-127
EN
Kinetin, BAP and NAA increased glucotropaeolin content in dry weight of nasturtium hairy roots but they inhibited biomass growth. Salicylates most strongly (by about 100-200% above control) and DL-?-aminobutyric acid and methyl jasmonate to a lesser degree increased glucotropaeolin yield. They also slightly increased myrosinase activity. Trans-cinnamic acid was a much stronger inducer of myrosinase activity than glucotropaeolin biosynthesis: it strongly inhibited biomass growth. L-1-amino-2-phenylethylphosphonic acid, inhibitor of L-phenylalanine ammonia-lyase (PAL), induced glucotropaeolin production and enhanced the effect of salicylates and jasmonate on glucotropaeoplin yield but it did not affect myrosinase activity.
12

Regeneration of Oxalis triangularis from hairy roots and the influence of glutathione on anthocyanin production

61%
Wielanek M., Urbanek H., Dobras M.
Biotechnologia
|
2004
|
issue 2
260-266
EN
The Oxalis triangularis transformed plants were regenerated from hairy roots induced by A. rhizogenes LBA 9402. In vitro shoot proliferation of the transformed roots-regenerated plants was 65% higher by than that of the non-transformed ones. In vivo the hairy root syndrome was observed for the transformed roots-regenerated plants. Anthocyanin content in the leaves of non-transformed plants was about 40% higher than in the transformed roots-regenerated ones. In the non-transformed plants, GSH (1,5 mM) enhanced anthocyanin production by 30% at the most, while in the transformed roots-regenerated plants the maximal increase in its production was found on the 2nd and 9th days after the treatment, it reached 230-320% and 750-600% of control, respectively.
first rewind previous Page / 1 next fast forward last
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.