THE EFFECT OF CHITOSAN HYDROLYSATE ON THE FUNGI ALTERNARIA SOLANI, FUSARIUM SOLANI AND RHIZOCTONIA SOLANI IN VITRO

• Authors: Balzhima Ts. Shagdarova , Natalia V. Karpova , Alla V. Il’ina , Valery P. Varlamov
• Languages of publication: EN

Abstracts

EN

Chitosan hydrolysate was obtained using nitric acid; the prevailing fraction had a molecular weight of 30 kDa and a deacetylation degree of 95%. The effect of chitosan hydrolysate when added to potato dextrose agar (PDA) in different concentrations (0.5, 1, 1.5, 2, 4, 6 and 8 mg/mL) was studied on the growth of the fungi Alternaria solani Sorauer, Fusarium solani (Mart.) Sacc. and Rhizoctonia solani J.G. Kühn. A. solani was the most sensitive to the addition of chitosan hydrolysate to PDA in radial growth experiments. On days 3 and 7 of incubation, the antifungal activity of the phytopathogen growth was 69%-92% and 69%-88%, respectively, in the concentration range of 0.5-2 mg/ml.

Keywords

EN

Alternaria solani; Fusarium solani; Rhizoctonia solani; chitosan; chitosan hydrolysate

• Publisher: Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
• Journal: Progress on Chemistry and Application of Chitin and its Derivatives
• Year: 2021
• Volume: 26
• Pages: 200-209

Contributors

author

Balzhima Ts. Shagdarova

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences.

author

Natalia V. Karpova

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences.

author

Alla V. Il’ina

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences.

author

Valery P. Varlamov

• Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences.

References

• Boudh S., Singh J.S.; (2018) Pesticide contamination: Environmental problems and remediation strategies. In: Emerging and Eco-Friendly Approaches for Waste Management. Springer Singapore, pp 245–269. DOI:10.1007/978-981-10-8669-4_12.
• Hassan O., Chang T.; (2017) Chitosan for eco-friendly control of plant disease. Asian J Plant Pathol 11, 53–70. https://doi.org/10.3923/ajppaj.2017.53.70
• Varlamov V.P., Il’ina A.V., Shagdarova B.Ts., Lunkov A.P., Mysyakina I.S.; (2020) Chitin/chitosan and its derivatives: fundamental problems and practical approaches. Biochemistry, 85 (1), S154-S176. DOI: 10.1134/S0006297920140084
• Goy R.C., Britto D.De., Assis O.B.G.; (2009) A review of the antimicrobial activity of chitosan. Polímeros 19, 241–247. https://doi.org/10.1590/S0104-14282009000300013
• Alburquenque C., Bucarey S.A., Neira-Carrillo A., Urzúa B., Hermosilla G., Tapia C.V.; (2010) Antifungal activity of low molecular weight chitosan against clinical isolates of Candida spp. Med Mycol 48, 1018–1023. https://doi.org/10.3109/13693786.2010.486412
• Xu J., Zhao X., Han X., Du Y.; (2007) Antifungal activity of oligochitosan against Phytophthora capsici and other plant pathogenic fungi in vitro. Pestic Biochem Physiol 87, 220–228. https://doi.org/10.1016/j.pestbp.2006.07.013
• Ait Barka E., Eullaffroy P., Clément C., Vernet G.; (2004) Chitosan improves development, and protects Vitis vinifera L. against Botrytis cinerea. Plant Cell Rep 22, 608–614. https://doi.org/10.1007/s00299-003-0733-3
• Xing K., Xing Y., Liu Y., Zhang Y., Shen X., Li X., Miao X., Feng Z., Peng X., Qin S; (2018) Fungicidal effect of chitosan via inducing membrane disturbance against Ceratocystis fimbriata. Carbohydr Polym 192, 95–103. https://doi.org/10.1016/j.carbpol.2018.03.053
• Verlee A., Mincke S., Stevens C.V.; (2017) Recent developments in antibacterial and antifungal chitosan and its derivatives. Carbohydr. Polym. 164, 268–283. DOI:10.1016/j.carbpol.2017.02.001.
• Olmstead R.G., Bohs L.; (2007) A summary of molecular systematic research in Solanaceae: 1982–2006. In: Acta Horticulturae. International Society for Horticultural Science, 255–268. DOI:10.17660/ActaHortic.2007.745.11.
• Bhattacharya A., College I.C.V., Tripura S.; (2013) Fungicidal potential of chitosan against phytopathogenic Fusarium solani. J Exp Biol Agric Sci 1, 259–263
• Chaerani R., Voorrips R.E.; (2006) Tomato early blight (Alternaria solani): the pathogen, genetics, and breeding for resistance. J Gen Plant Pathol 72, 335–347. https://doi.org/10.1007/s10327-006-0299-3
• Atiyah Q., Al-Tamimi A., Zugher Hussein H., Ali A.M.; (2020) The efficacy test of nano chitosan and phylex in resistance early blight disease in tomato caused by Alternaria solani Fungus. Int J Pharm Res 12, 2209–2220. https://doi.org/10.31838/ijpr/2020.12.01.345
• Mohammed S.R., Zeitar E.M., Eskov I.D.; (2019) Inhibition of mycelial growth of Rhizoctonia solani by chitosan in vitro and in vivo. Open Agric J 13, 156–161. https://doi.org/10.2174/1874331501913010156
• Quiterio-Gutiérrez T., Ortega-Ortiz H., Cadenas-Pliego G., Hernández-Fuentes A.D., Sandoval-Rangel A., Benavides-Mendoza A., Cabrera-De La Fuente M. (2019) The application of selenium and copper nanoparticles modifies the biochemical responses of tomato plants under stress by Alternaria solani. Int J Mol Sci 20, 1950. https://doi.org/10.3390/ijms20081950
• Shagdarova B.T., Ilyina A.V., Lopatin S.A., Kartashov M.I., Arslanova L.R., Dzhavakhiya V.G., Varlamov .P.; (2018) Study of the protective activity of chitosan hydrolyzate against septoria leaf blotch of wheat and brown spot of tobacco. Appl Biochem Microbiol 54:71–75. https://doi.org/10.1134/S0003683818010118
• Lopatin S.A., Derbeneva M.S., Kulikov S.N., Varlamov V.P., Shpigun O.A.; (2009) Fractionation of chitosan by ultrafiltration. J Anal Chem 64, 648–651. https://doi.org/10.1134/S1061934809060197
• Karpova N.V., Shagdarova B.T., Lyalina T.S., Il’ina A.V., Tereshina V.M., Varlamov V.P.; (2019) Influence of the main characteristics of low weight chitosan on the growth of the plant pathogenic fungus Botrytis сinerea. Appl Biochem Microbiol 55, 405–413. https://doi.org/10.1134/S0003683819040069
• Tsai G.J., Zhang S.L., Shieh P.L.; (2004) Antimicrobial activity of a low-molecularweight chitosan obtained from cellulase digestion of chitosan. J Food Prot 67, 396–398. https://doi.org/10.4315/0362-028X-67.2.396
• Taheri P., Gnanamanickam S., Höfte M.; (2007) Characterization, genetic structure, and pathogenicity of Rhizoctonia spp. associated with rice sheath diseases in India. Phytopathology 97, 373–383. https://doi.org/10.1094/PHYTO-97-3-0373
• Kumar M., Singh V., Singh K.N., Vikram P.; (2008) Morphological and virulence characterization of Rhizoctonia solani causing sheath blight of rice. Enviromental Ecol 26, 1158–1166
• Lal M., Kandhari J.; (2009) Cultural and morphological variability in Rhizoctonia solani isolates causing sheath blight of rice. IJAR 39, 77–81

• Document Type: article