Isolation and Identification of Potential Pathogenic Bacteria in Living Carp (Cyprinus carpio Linnaeus, 1758) Sold in Supermarkets in Cimahi City, Java

• Authors: Auryn Ramadhany Geraldine , Rosidah Rosidah , Heti Herawati , Ibnu Bangkit Bioshina
• Languages of publication: EN

Abstracts

EN

This research was conducted with the aim to analyze the presence of potential pathogenic bacteria in carp that are sold live in supermarkets in the city of Cimahi and to find out the species of these bacteria. Fish samples were obtained from two supermarkets in Cimahi City, Transmart and Superindo. From each supermarket three fish samples were taken once a week and repeated three times. Bacteria were isolated from several parts of the fish body namely body surface mucus, gills, liver, and kidneys. The results of isolation from each target organ were biochemically tested to determine the species of bacteria. Potential pathogenic bacteria found in carp from this research are Aeromonas hydrophila, Aeromonas sobria, Aeromonas schubertii, Aeromonas media, Aeromonas caviae, Aeromonas ecrenophila, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Pseudomonas aeruginosa, Aeromonas caviae, Aeromonas ecrenophila, Pseudomonas stutzeri, Pseudomonas pseudoalcaligenes, Pseudomonas aeruginosa, Plesiomonas shigelloides, Bacillus megaterium, Bacillus thuringiensis, Neisseria mucosa, Citrobacter freundii, Serratia marcescens, Staphylococcus epidermidis, Micrococcus luteus, Enterobacter aerogenes, and Enterobacter sakazakii.

Keywords

EN

Cyprinus carpio; Isolation and identification; Pathogenic bacteria; Supermarket

Discipline

• FISHERIES: FISHERIES

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2020
• Volume: 32
• Pages: 21-35

Contributors

author

Auryn Ramadhany Geraldine

• Faculty of Fisheries and Marine Science, Padjajaran University, Indonesia

author

Rosidah Rosidah

• Faculty of Fisheries and Marine Science, Padjajaran University, Indonesia

author

Heti Herawati

• Faculty of Fisheries and Marine Science, Padjajaran University, Indonesia

author

Ibnu Bangkit Bioshina

• Faculty of Fisheries and Marine Science, Padjajaran University, Indonesia

References

• [1] Kisbiyanto, Arif. 2013. Socio-Economic Impacts of Modern Market Existence in Traditional Markets (Study on the City of Boyolali Market). Yogyakarta State University.
• [2] Jasminandar. 2011. Prevalence of Parasites and Diseases of Freshwater Fish Cultivated in Kupang City / Regency. Journal of Life and Physical Sciences Vol. 13, No. 1, March 2011: 25-30
• [3] Lubis, YPP, Yunasfi, and R. Leidonald. 2014. Types of bacteria in catfish wound. Journal of Aquacostamarine 2 (1): 66-77
• [4] World Health Organization. 2004. The World Health Organization Quality of Life (WHOQOL) –BREF
• [5] Susatyo, I.D. 2006. Isolation and Identification of Gelatinolytic Bacteria from the Ponds of Gresik and Lamongan Regions. Airlangga University, Surabaya.
• [6] Pękala, Agnieszka and Safińska. 2018. Contemporary Threats of Bacterial Infections in Freshwater Fish. J Vet Res 62: 261-267
• [7] Kivanc, M., Yilmaz, M., and Demir, F. 2011. The Occurrence of Aeromonas In Drinking Water, Tap Water And The Porsuk River. Brazilian Journal of Microbiology 42: 126-131
• [8] Haryani, A., R. Grandiosa, ID Buwono, and A. Santika. 2012. Test the effectiveness of papaya leaves (Carica papaya) for the treatment of Aeromonas hydrophila bacterial infections in goldfish (Carassius auratus). Journal of Fisheries and Maritime Affairs, 3 (3): 213-220.
• [9] Lukistyowati, I. and Morina R. 2005. Analysis of Fish Diseases. UNRI-Press, Pekanbaru. 120 p.
• [10] Miller, W.A., Miller, M.A., Gardner, I.A., Atwill, E.R., and Byrne, B.A. 2006. Salmonella spp., Vibrio spp., Clostridium perfringens, and Plesiomonas shigelloides in Marine and Freshwater Invertebrates from Coastal California Ecosystems. Microb Ecol 52: 198-206
• [11] Angreni, NPW, Arthana, IW, and Wulandari, E. 2018. Distribution of Pathogenic Bacteria in Tilapia (Oreochromis niloticus) in Lake Batur, Bali. Current Trends in Aquatic Science I (I): 96-103
• [12] Kubilay, A. and Ulukoy, G. 2004. First isolation of Staphylococcus epidermidis from cultured gilthead sea bream (Sparus aurata) in Turkey. Bull. Eur. Ass. Fish Pathol 24(3): 137
• [13] Otto, M. 2009. Staphylococcus epidermidis – The “Accidental” Pathogen. Nat Rev Microbiol 7(8): 555-567
• [14] Gunn, B.A., Singleton F.L., Peele E.R., and Colwell, R.R. 1982. A note on the isolation and enumeration of Gram-positive cocci from marine and estuarine waters. Journal of Applied Bacteriology 53: 127-129
• [15] Austin, B. and Austin, D.A. 1999. Bacterial Fish Pathogens. Diseases of Farmed and Wild Fish, 3rd edn. Springer-Praxis Publ. London. 411 p.
• [16] Syihab, MIMT, Suryanto, D., Harahap, ZA, and Dhuha, OR. 2015. Bacteria on the Body of Gouramy Fish (Osphronemus gouramy) Due to Infestation of Etoparasarasite Argulus sp. University of Northern Sumatra. Field.
• [17] Aydin, S., A. Ciltas, H. Yetim, and I. Akyurt. 2005. Clinical, Pathological and Haematological Effects of Micrococcus luteus Infections in Rainbow Trout (Oncorhynchus mykiss Walbaum). Journal of Animal and Veterinary Advances 4 (2): 167-174.
• [18] Cowan, S.T. 2004. Manual for the Identification of Medical Fungi. Cambridge University Press, London.
• [19] Neto, Rodrigues J., Yano T., Beriam L.O.S., Destéfano S.A.L., Oliveira V.M., and Rosato Y.B. 2003. Comparative RFLP-Its Analysis Between Enterobacter Cloacae Strains Isolated from Plants and Clinical Origin. Arq Inst Biol 70: 367-72
• [20] Karsinah. 2004. Detection of Salmonella. Airlangga University, Surabaya.
• [21] Kozińska, A. and Pękala, A. 2004. First Isolation of Shewanella putrefaciens From Freshwater Fish – a Potential New Pathogen of The Fish. Bull. Eur. Ass. Fish Pathol 24(4): 199
• [22] Svetlana, J., Dobrila, J. D., and Veljovic. (2003). Citrobacter freundii as a cause of Disease in Fish. Acta Veterinaria 53, 5-6
• [23] Paździor, Ewa. 2016. Shewanella putrefaciens – a New Opportunistic Pathogen of Freshwater Fish. J Vet Res 60: 429-434
• [24] Dalahi, F., Subekti, S., and Agustono. 2014. Isolation and Identification of Bacteria in the Digestive Tract of Gouramy Fish (Osphronemus gouramy) With Different Commercial Feeds. Journal of Fisheries and Marine Scientific 6 (1): 87-92
• [25] Flyg, C. and Xanthopoulos, G.K. 1983. Insect Phatogenic Properties of S. marcescens Passive and Active Resistance to Insect Immunity Studied with Protease Deficient and Phage-Resistance Mutants. Journal of General Microbiology 129: 453-464
• [26] Liu, G., Tang, C.M., and Exley, R.M. 2015. Non-pathogenic Neisseria: members of an abundant, multi-habitat, diverse genus. Microbiology 161: 1297-312

• Document Type: article