Early utilization of Spirulina platensis cultivation as an antioxidant candidate in laboratory-scale closed reactor system

• Authors: Dewi Oktaviani , Asep Hasan Basri , Windi Damayanti
• Languages of publication: EN

Abstracts

EN

Spirulina platensis is a microalga that contains a lot of secondary metabolites and is utilized as a dietary supplement. It can inhibit fat peroxidation better compared to the chemical antioxidant. Laboratory-scale closed reactor system was designed for Spirulina cultivation. This research aims to analyze identified secondary metabolite (Phytochemical contents) and the antioxidant activity or value of Inhibition Concentration 50% (IC50) of the fresh lab-scale cultivated Spirulina platensis. The antioxidant content was evaluated using DPPH method (1,1–diphenyl-2-pikrilhidrazil) on various sample concentrations as well as using Vc (Ascorbic Acid) as a positive control. The principle of hydrogen absorption by free radicals from antioxidants was demonstrated with the absorption value using a spectrophotometer at a wavelength of 517 nm. The results show that Spirulina platensis lab-scale cultivation was resulting in 5.0±0.01 g/10.0 L wet basis on the 10th day. The results show that Spirulina platensis has positive compounds of flavonoid, steroids, triterpenoids, phenolic, and saponins. Fresh Spirulina platensis has a value of IC50 647.045 ppm and Vc as a positive control has the IC50 of 2.085 ppm. The potential as a source of natural antioxidants was categorized as a very weak capacity. Therefore this study can be concluded that Spirulina platensis cultivated in Laboratory-scale has a potential to act as antioxidant candidate.

Keywords

EN

Antioxidant; DPPH; Fresh Spirulina; Laboratory-scale; Spirulina platensis

Discipline

• AQUATIC_SCIENCES: AQUATIC SCIENCES

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2021
• Volume: 34
• Pages: 144-153

Contributors

author

Dewi Oktaviani

• Faculty of Fisheries and Marine Science, University Padjadjaran, Bandung-Sumedang St. KM.21, 45363 West Java, Indonesia

author

Asep Hasan Basri

• Faculty of Fisheries and Marine Science, University Padjadjaran, Bandung-Sumedang St. KM.21, 45363 West Java, Indonesia

author

Windi Damayanti

• Faculty of Fisheries and Marine Science, University Padjadjaran, Bandung-Sumedang St. KM.21, 45363 West Java, Indonesia

References

• [1] Czerwonka, A., Kaławaj, K., Sławińska-Brych, A., Lemieszek, M.K., Bartnik, M., Wojtanowski, K.K., Zdzisińska, B., Rzeski, W. Anticancer effect of the water extract of a commercial Spirulina (Arthrospira platensis) product on the human lung cancer A549 cell line. Biomedicine and Pharmacotherapy, 106 (2018) 292–302. https://doi.org/10.1016/j.biopha.2018.06.1
• [2] Firdiyani F., Agustini T.W., Ma’ruf W.F. Extraction of bioactive compounds as natural antioxidants from fresh spirulina platensis using different solvents. (Ekstraksi Senyawa BIoaktif sebagai Antioksidan Alami Spirulina Platensis Segar dengan Pelarut yang Berbeda). Jurnal Pengolahan Hasil Perikanan Indonesia, 18(1) (2015) 28–37. https://doi.org/10.17844/jphpi.2015.18.1.28 (In Indonesian Language).
• [3] Hirata T., Mikiya T., Masaki O., Teppei T., Morihiko S. Antioxidant activities of phycocyanobilin prepared from Spirulina platensis. Journal of Applied Phycology, 12(3–5) (2000) 435–439. https://doi.org/10.1023/a:1008175217194
• [4] Huesemann, M., Crowe, B., Waller, P., Chavis, A., Hobbs, S., Edmundson, S., Wigmosta, M. A validated model to predict microalgae growth in outdoor pond cultures subjected to fluctuating light intensities and water temperatures. Algal Research, 13 (2016) 195–206. https://doi.org/10.1016/j.algal.2015.11.008
• [5] Karkos, P.D., Leong, S.C., Karkos, C.D., Sivaji N., Assimakopoulos D.A. Spirulina in clinical practice: Evidence-based human ap.plications. Evidence-Based Complementary and Alternative Medicine, (2011). Volume 2011, Article ID 531053. https://doi.org/10.1093/ecam/nen058
• [6] Molyneux, P. The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin Journal of Science and Technology, 26 (2004) 211–219. https://doi.org/10.1287/isre.6.2.144
• [7] P D Karkos, S C Leong, C D Karkos, N Sivaji, D A Assimakopoulos. Spirulina in clinical practice: evidence-based human applications. Evid Based Complement Alternat Med. 2011; 2011: 531053. doi: 10.1093/ecam/nen058
• [8] Nuhu, A.A. Spirulina (Arthrospira): An Important Source of Nutritional and Medicinal Compounds. Journal of Marine Biology, (2013), Article ID 325636, 1–8. https://doi.org/10.1155/2013/325636
• [9] Pérez-López, P., Ledda, F.D., Bisio, A., Feijoo, G., Perino, E., Pronzato, R., Manconi, R., and Moreira, M.T. Life cycle assessment of in situ mariculture in the Mediterranean Sea for the production of bioactive compounds from the sponge Sarcotragus spinosulus. Journal of Cleaner Production, 142 (2017) 4356–4368. https://doi.org/10.1016/j.jclepro.2016.11.137
• [10] Ramanna, L., Rawat, I., Bux, F. Light enhancement strategies improve microalgal biomass productivity. Renewable and Sustainable Energy Reviews, 80 (2017) 765–773. https://doi.org/10.1016/j.rser.2017.05.202
• [11] Santos-sánchez, N.F., Salas-coronado, R., Valadez-blanco, R., Hernández-carlos, B., Guadarrama-mendoza, P.C. Natural antioxidant extracts as food preservatives. Acta Scientiarum Polonorum Technologia Alimentaria, 16(4) (2017) 361–370. http://dx.doi.org/10.17306/J.AFS.2017.0530
• [12] Sidi, N., Aris, A.Z., Mohamat, F., Looi, L.J. Tape seagrass (Enhalus acoroides) as a bioindicator of trace metal contamination in Merambong shoal, Johor Strait, Malaysia. Marine Pollution Bulletin Volume 126, January 2018, Pages 113-118. https://doi.org/10.1016/j.marpolbul.2017.10.041
• [13] Soni, A.R., Sudhakar, K., Rana, R.S. Spirulina - From growth to nutritional product : A review. Trends in Food Science and Technology, 69 (2017) 157–171. https://doi.org/10.1016/j.tifs.2017.09.010
• [14] Soni, E.A., Sudhakar, K., Rana, R.S. Comparative study on the growth performance of Spirulina platensis on modifying culture media. Energy Reports, 5 (2019) 327–336. https://doi.org/10.1016/j.egyr.2019.02.009
• [15] Teng, H., Lee, W.Y. Antibacterial and antioxidant activities and chemical compositions of volatile oils extracted from Schisandra chinensis Baill. seeds using simultaneous distillation extraction method , and comparison with Soxhlet and microwave-assisted extraction. Bioscience, Biotechnology and Biochemistry, 78(1) (2014) 79–85. https://doi.org/10.1080/09168451.2014.877815
• [16] Wang, C., Fu, C., Liu, Y. Effects of using light-emitting diodes on the cultivation of Spirulina platensis. Biochemical Engineering Journal Volume 37, Issue 1, 15 October 2007, Pages 21-25. https://doi.org/10.1016/j.bej.2007.03.004
• [17] Wikanta, T., Januar, H.I., and Nursid, M. Assay activity antioxidant, toxicity and cytotoxicity red algae extract Rhodymenia palmate Jurnal Penelitian Perikanan Indonesia, 11(4) (2017) 41. https://doi.org/10.15578/jppi.11.4.2005.41-49
• [18] Ye, C., Mu, D., Horowitz, N., Xue, Z., Chen, J., Xue, M. Life cycle assessment of industrial scale production of spirulina tablets. Algal Research, 34 (2018) 154–163. https://doi.org/10.1016/j.algal.2018.07.013

• Document Type: article