Microbial Contaminants and Aflatoxin Level in Indigenous Okra (Abelmoschus esculentus), Bologi (Senecio biafrae) and Ugwu (Telfairia occidentalis) from Two Selected Markets in Oyo State, Nigeria

• Authors: U. A. Oluoyeneye , E. O. Oni , O. D. Umoren , N. B. Ogunsami
• Languages of publication: EN

Abstracts

EN

Farm product are majorly consumed globally, however, their level of contamination from microbes and aflatoxins from fungi species is worrisome. This study aimed to study the microbes and aflatoxin level in Okra (Abelmoschus esculentus), Ugwu (Telfairia occidentalis) and Bologi (Senecio biafrae) from two selected markets (Bodija and Ojoo) in Oyo State. Thirty (30) fresh samples of Okra, Ugwu and Bologi were purchased from the study area. Microbiological examination was carried out using standard microbiological procedures, aflatoxin quantification was assayed using High Performance Liquid Chromatography while aflatoxigenic potential of the fungal isolates was carried out on neutral red desiccated coconut agar. The result revealed the presence of six bacterial isolates (Proteus mirabilis, Micrococcus luteus, Klebsiella pneumonia, Streptococcus spp., Bacillus cereus and Salmonella sp.) and four (4) fungal isolates (Aspergillus niger, Aspergillus parasiticus, Cladosporium sp. and Rhizopus sp.) in the samples. The highest level of aflatoxin was observed in dried okra (14.000 µg/kg) from Bodija Market, while the lowest level was found in fresh Ugwu (0.001 µg/kg) from Ojoo Market. AFB1 was detected in all samples except for the fresh and dry Ugwu from Ojoo Market and the dry Ugwu from Bodija Market. Additionally, AFB2 was found in all samples. The non-aflatoxigenic fungi included Aspergillus niger found in okra from Ojoo Market, Aspergillus flavus in Ugwu from Bodija Market, and Aspergillus parasiticus in Ugwu from Ojoo Market. In contrast, Aspergillus parasiticus in okra from Bodija Market is aflatoxigenic. In conclusion, the vegetables investigated from the selected markets are contaminated with bacteria and fungi, except for Bologi, and aflatoxigenic fungus was identified in okra from Bodija Market.

Keywords

EN

Aflatoxins; HPLC; Microbial Contaminants; Vegetables; bologi; okra; ugwu

Discipline

• MICROBIOLOGY_&_CELL_BIOLOGY: MICROBIOLOGY & CELL_BIOLOGY

• Publisher: Scientific Publishing House „DARWIN”
• Journal: World News of Natural Sciences
• Year: 2025
• Volume: 60
• Pages: 195-204

Contributors

author

U. A. Oluoyeneye

• Department of Microbiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

author

E. O. Oni

• Department of Microbiology, Federal University of Agriculture, Abeokuta, Ogun State, Nigeria

author

O. D. Umoren

• Department of Biological Sciences, National Open University of Nigeria, Abuja, Nigeria

author

N. B. Ogunsami

• Department of Biology Education, Lagos State University, Ojo, Lagos State, Nigeria

References

• [1] Adebayo-Tayo, B. C., & Odu, N. N. (2018). Fungal contamination and aflatoxin levels in stored indigenous vegetables. Environmental Biotechnology Research, 3(2), 112-120
• [2] Atanda, O., Ogunrinu, M., & Olorunfemi, F. (2011). A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins. World Mycotoxin Journal, 4(2), 147-155
• [3] Bensch, K., Groenewald, J. Z., Meijer, M., Dijksterhuis, J., Jurjević, Ž., Andersen, B. & Samson, R. A. (2018). Cladosporium species in indoor environments. Studies in Mycology, 89, 177-301
• [4] Hell, K., Cardwell, K. F., Setamou, M., & Poehling, H. M. (2000). The influence of storage practices on aflatoxin contamination in maize in four agroecological zones of Benin, West Africa. Journal of Stored Products Research, 36(4), 365-382
• [5] Ishola, T. M., Adebiyi, A. O., & Alaka, O. O. (2020). Microbial contamination and aflatoxin occurrence in selected indigenous vegetables from southwest Nigeria. Journal of Food Safety and Hygiene, 6(1), 45-52
• [6] Malik, I.F., Musa, I., Musa, A.I., Bitrus, J.N., Emmanuel, O.M., Mubarak, B.S. & Umoren, O.D. (2024). Concentration and Health Risk Assessment of Selected Heavy Metals (HMs) in African spinach (Amaranthus hybridus) and Tomato (Solanum lycopersicum) Grown around Ashaka Community, Gombe State, Nigeria. Journal of Chemistry and Nutritional Biochemistry 5(2), 16-26. https://doi.org/10.48185/jcnb.v5i2.1175
• [7] Onakpa, M. M., Abubakar, S. S., & Obioha, C. O. (2021). Comparative study on microbial and aflatoxin contamination of selected indigenous vegetables. African Journal of Biotechnology, 20(4), 78-85
• [8] Oni, E. O., Adebajo, L. O., Badmos, A. O., Adeleye, T. M., Oyeyipo, F. M. & Adebiyi, G. E. (2019). Mycoflora and Aflatoxin Levels in Stale Retailed Pepper Sold in Abeokuta Nigeria. Nigerian Journal of Biotechnology 36 (2), 21-26
• [9] Oni, E. O., Oni, N. O., Bamidele, J., Taiwo, O. A., Oyetibo, O. B., Badmos, A. O., et al. (2023). Microbial load and aflatoxin contamination in locally formulated herbal mixtures obtained from Itoku market, Nigeria. J Food Safe & Hyg. 9(3): 197-206
• [10] Schmidt-Heydt, M., Abdel-Hadi, A., Magan, N., & Geisen, R. (2009). Complex regulation of the aflatoxin biosynthesis gene cluster of Aspergillus flavus in relation to various combinations of water activity and temperature. International Journal of Food Microbiology, 135(3), 231-237
• [11] Udomkun, P., Wiredu, A. N., Nagle, M., Bandyopadhyay, R., Müller, J., & Vanlauwe, B. (2017). Innovative technologies to manage aflatoxins in foods and feeds. International Journal of Environmental Research and Public Health, 14(9), 999
• [12] Umoren, O.D., Adu, O.M., Oni, C.C., Olukotun, S.T.A., Iche, L.C. & Atunwa, B.O. (2024). Communicating the Parasitic Contaminants in Readily Consumed Fruits Sold in Abeokuta, Ogun State Nigeria and its Public Health Implications. FUAM Journal of Pure and Applied Sciences 4(2), 99-106
• [13] Umoren, O.D., Makinwa, P.T., Alani, O.A., Anyanwu, G.O., Anyanwu, L.I., Oribhabor, I., Afolabi, T. & Oluwadare, O.J. (2025). Potential Health Risk of Exposure to Microbial Contaminants in Settled Indoor Dusts (SIDs) from a Polytechnic Environment. FUAM Journal of Pure and Applied Sciences 5(1), 23-29
• [14] Visagie, C. M., Hirooka, Y., Tanney, J. B., Whitfield, E., Mwange, K., Meijer, M. & Samson, R. A. (2014). Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world. Studies in Mycology, 78, 63-139
• [15] Owoeye, A. L., Laurie, M. C., Allagheny, N. N., & Onyezili, F. N. (1990). Chemical and physical parameters affecting the viscosity of mixed okra and tomato homogenate. Journal of the Science of Food and Agriculture, 53, 283–286. doi:10.1002/jsfa.2740530218
• [16] Elkhalifa, A. E. O., Alshammari, E., Adnan, M., Alcantara, J. C., Awadelkareem, A. M., Eltoum, N. E., Mehmood, K., Panda, B. P., & Ashraf, S. A. (2021). Okra (Abelmoschus Esculentus) as a Potential Dietary Medicine with Nutraceutical Importance for Sustainable Health Applications. Molecules, 26(3), 696. https://doi.org/10.3390/molecules26030696
• [17] Nipaporn Sengkhamparn, Leonard M.C. Sagis, Renko de Vries, Henk A. Schols, Tanaboon Sajjaanantakul, Alphons G.J. Voragen. Physicochemical properties of pectins from okra (Abelmoschus esculentus (L.) Moench). Food Hydrocolloids, Volume 24, Issue 1, 2010, Pages 35-41, https://doi.org/10.1016/j.foodhyd.2009.07.007
• [18] Adebayo AG: Inventory of antidiabetic plants in selected districts of Lagos State, Nigeria. J Ethnopharmacol. 2009, 121: 135-139. 10.1016/j.jep.2008.10.013
• [19] Lienou, L.L., Telefo, B.P., Bale, B. et al. Effect of the aqueous extract of Senecio biafrae (Oliv. & Hiern) J. Moore on sexual maturation of immature female rat. BMC Complement Altern Med 12, 36 (2012). https://doi.org/10.1186/1472-6882-12-36
• [20] Akoroda, M.O. Ethnobotany ofTelfairia occidentalis (cucurbitaceae) among Igbos of Nigeria. Econ Bot 44, 29–39 (1990). https://doi.org/10.1007/BF02861064
• [21] Kayode Esuoso, Harald Lutz, Mohamed Kutubuddin, Ernst Bayer. Chemical composition and potential of some underutilized tropical biomass. I: fluted pumpkin (telfairia occidentalis). Food Chemistry, Volume 61, Issue 4, 1998, Pages 487-492, https://doi.org/10.1016/S0308-8146(97)00096-4
• [22] WHO, (2018). Aflatoxins. Food Safety Digest. Ref. No; WHO/NHM/FOS/RAM/18.1. Department of food safety and zoonoses. World health Organization (Accessed January 2024).

• Document Type: article