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Number of results
2010 | 15 | 197 - 204

Article title

KINETICS OF THERMAL DEACTIVATION OF CHITIN DEACETYLASE

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EN

Abstracts

EN
In the paper the thermal deactivation of chitin deacetylase separated from Absidia orchidis vel coerulea was investigated. Experiments were carried out at a temperature of 50ºC up to 24 hours. Three mechanisms of deactivation were tested: irreversible first-order, reversible firstorder, and three-parameters phenomenological model. It was found that the last one gave the best approximation of the deactivation process. The parameters of the model were evaluated and the model was also confirmed by independent experiments.

Contributors

  • Faculty of Chemical and Process Eng. Warsaw University of Technology

References

  • Tokuyasu K., Ohnishi-Kameyama M., Hayashi K.; 1996 “Purification and characterization of extracellular chitin deacetylase from Colletotrichum lindemutianum”, Biosci. Biotech. Biochem. 60, pp. 1598-1603.
  • Amorim R. V. S., Ledingham W. M., Fukushima K., Campos-Takaki G. M.; J. Ind. Microbiol. Biotechnol. 2005, 32, pp. 19-23.
  • Cai J., Yang J., Du Y., Du Y., Fan L., Qiu Y., Kennedy J. F.; Purification and characterization of chitin deacetylase from Scopulariopsis brevicaulis. Carbohyd. Polym. 2006, 65, pp. 211-217.
  • Kołodziejska I., Malesa-Ciećwierz M., Lerska A., Sikorski Z.; 1999 „Properties of chitin deacetylase from crude extracts of Mucor rouxii”, J Food Biochem. 23, pp. 45-57.
  • Martinou A., Kafetzopoulos D., Bouriotis V.; 1995 “Chitin deacetylation by enzymatic means: monitoring of deacetylation process”, Carbohydrate Res. 273, pp. 235-242.
  • Jaworska M. M.; Konieczna E.; The influence of supplemental components in nutrient medium on chitosan formation by the fungus Absidia orchidis Appl. Microbiol.Biotechnol., 2001, 56, pp. 220-224.
  • Domszy J. G., Roberts G. A. F.; 1985. Evaluation of infrared spectroscopic techniques for analyzing chitosan. Makromol Chem, 186, pp. 1671-1673.
  • Sadana A.; Biocatalysis, Fundamentals of Enzyme Deactivation Kinetics, Prentice-Hall Inc. 1991.
  • Aymard Ch., Belarbi A.; Kinetics of thermal deactivation of enzymes: a simple three parameters phenomenological model can describe the decay of enzyme activity, irrespectively of the mechanism., Enz Microb Tech 2000, 27, pp. 642-618.
  • Jaworska M., Konieczna E.; Kinetics of enzymatic deacetylation of chitosan, Inz. Chem Proc. 2004, 25, pp. 1029-1034.

Document Type

article

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.psjd-60a1f8c9-9dd3-4358-86d5-33773d79e8fa
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