DELAYED URIC ACID ACCUMULATION IN PLASMA PROVIDES ADDITIONAL ANTIOXIDANT PROTECTION AGAINST IRONTRIGGERED OXIDATIVE STRESS AFTER A WINGATE TEST

• Authors: TP Souza-Junior , L. Lorenço-Lima , D. Ganini , CV Vardaris , TG Polotow , MP Barros
• Languages of publication: EN

Abstracts

EN

Reactive oxygen species are produced during anaerobic exercise mostly by Fe ions released into plasma and endothelial/muscle xanthine oxidase activation that generates uric acid (UA) as the endpoint metabolite. Paradoxically, UA is considered a major antioxidant by virtue of being able to chelate pro-oxidative iron ions. This work aimed to evaluate the relationship between UA and plasma markers of oxidative stress following the exhaustive Wingate test. Plasma samples of 17 male undergraduate students were collected before, 5 and 60 min after maximal anaerobic effort for the measurement of total iron, haem iron, UA, ferric-reducing antioxidant activity in plasma (FRAP), and malondialdehyde (MDA, biomarker of lipoperoxidation). Iron and FRAP showed similar kinetics in plasma, demonstrating an adequate pro-/antioxidant balance immediately after exercise and during the recovery period (5-60 min). Slight variations of haem iron concentrations did not support a relevant contribution of rhabdomyolysis or haemolysis for iron overload following exercise. UA concentration did not vary immediately after exercise but rather increased 29% during the recovery period. Unaltered MDA levels were concomitantly measured. We propose that delayed UA accumulation in plasma is an auxiliary antioxidant response to post-exercise (ironmediated) oxidative stress, and the high correlation between total UA and FRAP in plasma (R-Square = 0.636; p = 0.00582) supports this hypothesis.

Keywords

EN

anaerobic metabolism; exercise; haemoglobin; lipid peroxidation; xanthine oxidase

Discipline

• MEDICINE: MEDICINE

• Publisher: Instytut Sportu
• Journal: Biology of Sport
• Year: 2014
• Volume: 31
• Issue: 4
• Pages: 271-276

Dates

published

2014

Contributors

author

TP Souza-Junior

author

L. Lorenço-Lima

author

D. Ganini

author

CV Vardaris

author

TG Polotow

author

MP Barros

References

• 1. Cooper CE, Vollaard NB, Choueiri T, Wilson MT. Exercise, free radicals and oxidative stress. Biochem Soc Trans. 2002;30(2):280-285.
• 2. Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: a review. Can J Appl Physiol. 2004;29(3):245- 263.
• 3. Cirrik S, Yavuzer BU, Öner G.Exercise- induced changes in renal URAT1 activity and expression in rats. Renal Fail. 2010;32(7):855-862.
• 4. Dotan R, Baror O.Load optimization for the wingate anaerobic test. Eur J Appl Physiol Occup Physiol. 1983;51(3):409- 417.
• 5. Martinovic J, Dopsaj V, Kotur-Stevuljević J, Dopsaj M, Nesic G. Oxidative stress status in elite female volleyball athletes with depleted iron stores. Br J Sports Med. 2011;45:534-535. doi:10.1136/ bjsm.2011.084558.5
• 6. Smith DJ, Roberts D.Effects of high volume and/or intense exercise on selected blood chemistry parameters. Clin Biochem. 1994;27(6):435-440.
• 7. DellaValle DM, Haas JD. Iron status is associated with endurance performance and training in female rowers. Med Sci Sports Exerc. 2012;44(8):1552-1559.
• 8. Teixeira V, Valente H, Casal S, Marques F, Moreira P.Antioxidant status, oxidative stress, and damage in elite trained kayakers and canoeists and sedentary controls. Int J Sport Nutr Exerc Metab. 2009;19(5):443-456.
• 9. Fogarty MC, Hughes CM, Burke G, Brown JC, Davison GW. Acute and chronic watercress supplementation attenuates exercise-induced peripheral mononuclear cell DNA damage and lipid peroxidation. Brit J Nutrit. 2013;109(2):293-301.
• 10. Morales-Alamo D, Calbet JAL. Free radicals and sprint exercise in humans. Free Radic Res2014; 48(1):30-42.
• 11. Bloomer RJ, Fisher-Wellman KH. Blood oxidative stress biomarkers: influence of sex, exercise training status, and dietary intake. Gender Med. 2008;5(3):218- 228.
• 12. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘Antioxidant Power’’: The FRAP assay. Anal Biochem. 1996;239(1):70-76.
• 13. Van Kampen EJ, Zijlstra WG. Determination of hemoglobin and its derivatives. Adv Clin Chem. 1965;8:141-187.
• 14. Khoschsorur GA, Winklhofer-Roob BM, Rabl H, Auer TH, Peng Z, Schaur RJ. Evaluation of a sensitive HPLC method for the determination of malondialdehyde, and application of the method to different biological materials. Chromatographia. 2000;52(3-4):181-184.
• 15. Beard J, Tobin B.Iron status and exercise. Am J Clin Nutr2000;72(2 Suppl):594S–597S.
• 16. Otocka-Kmiecik A, Lewandowski M, Stolarek R, Szkudlarek U, Nowak D, Orlowska-Majdak M.Effect of single bout of maximal exercise on plasma antioxidant status and paraoxonase activity in young sportsmen. Redox Rep. 2010;15(6):275-281.
• 17. Fernandez-Gonzalo R, Lundberg TR, Alvarez-Alvarez L, de Paz JA. Muscle damage responses and adaptations to eccentric-overload resistance exercise in men and women. Eur J Appl Physiol. 2014;114(5):1075-1084.
• 18. Kabasakalis A, Tsalis G, Zafrana E, Loupos D, Mougios V.Effects of endurance and high-intensity swimming exercise on the redox status of adolescent male and female swimmers. J Sports Sci. 2013; 32(8):747-756.
• 19. Kadiiska MB, Gladen BC, Baird DD, Germolec D, Graham LB, Parker CE, Nyska A, Wachsman JT, Ames BN, Basu S, Brot N, FitzGerald GA, Floyd RA, George M, Heinecke JW, Hatch GE, Hensley K, Lawson JA, Marnett LJ, Morrow JD, Murray DM, Plastaras J, Roberts II LJ, Rokach J, Shigenaga MK, Sohal RS, Sun J, Tice RR, Van Thiel DH, Wellner D, Walter PB, Tomer KB, Mason RP, Barrett JC. Biomarkers of oxidative stress - Study II. Are oxidation products of lipids, proteins, and DNA markers of CCl4 poisoning? Free Radic Biol Medic. 2005;38(6):698-710.

• Document Type: article