Diagnostic performance of loop-mediated isothermal amplification (LAMP) and Ultra-sensitive PCR in diagnosis of malaria in western Saudi Arabia

• Authors: Rasha H. Solimam , Alexandra Martin-Ramirez , Jose M. Rubio , Eman A. Khalifa , Bushra E. Hussein , Maha M. Wahab , Marta Lanza , Yousry A. Hawash

Abstracts

EN

Malaria diagnosis continues to be one of the most important steps in the cycle of control specially in endemic countries with low parasitic load infections. Loop-mediated isothermal amplification (LAMP) and ultrasensitive PCR (Us-PCR) are two promising candidates for malaria diagnosis. A cross sectional study performed at King Faisal Hospital, Taif KSA involved patients suffering from signs and symptoms suggesting of malaria, 35 blood samples diagnosed by Nested Multiplex PCR as a reference method (13 P. falciparum, 17 P. vivax, 3 mixed P. falciparum and P. vivax) plus two negative controls were selected to be included in this study to analyse the performance of two LAMP methods (LAMP OptiGene® and LAMP WarmStart®) and two ultrasensitive PCRs (Us-PCR TARE-2 and Us-PCR Var-ATS). LAMP OptiGene® and LAMP WarmStart® performances were identical and better than the performance of Us PCR TARE 2 and Us-PCR var-ATS for P. falciparum, achieving 93.75% sensitivity, 100% specificity and 97.17% accuracy versus 87.5% sensitivity, 100% specificity and 94.29% accuracy for the Us PCR TARE 2 and 81.25% sensitivity, 94.74% specificity and 88.57% accuracy for the Us PCR var-ATS respectively. In P. vivax diagnosis LAMP OptiGene® performed excellently with 100% sensitivity, specificity, and accuracy while LAMP WarmStart® and Us-PCR Cox1 achieved 100% sensitivity, specificity 93.33% and 97.14% accuracy. The study results highlighted the benefits of using LAMP techniques for field diagnosis of malaria in different settings where the need for a more sensitive and reliable molecular tool is mandatory but at the same time removing the high cost, long turnaround time and the need of highly specialized trained technicians to perform more sophisticated molecular techniques.

Keywords

EN

malaria; LAMP; ultrasensitive PCR; diagnosis

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2023
• Volume: 62
• Pages: 15-23

Dates

published

2023

Contributors

author

Rasha H. Solimam

• Department of Microbiology, College of Medicine, Taif University

• https://orcid.org/0000000234476512

author

Alexandra Martin-Ramirez

• Malaria & Emerging Parasitic Diseases Laboratory, National Microbiology Center, Instituto de Salud Carlos III

author

Jose M. Rubio

• Malaria & Emerging Parasitic Diseases Laboratory, National Microbiology Center, Instituto de Salud Carlos III
• Centro de Investigación Biomédica en Red de Enfermedades Infecciosas, CIBERINFEC

author

Eman A. Khalifa

• Department of Microbiology, College of Medicine, Taif University

author

Bushra E. Hussein

• Department of Tropical Medicine and Infectious Diseases, Faculty of Medicine, University of Tanta

author

Maha M. Wahab

• Microbiology and Parasitology Department, KFMC Taif, KSA

author

Marta Lanza

• Malaria & Emerging Parasitic Diseases Laboratory, National Microbiology Center, Instituto de Salud Carlos III

author

Yousry A. Hawash

• Department of Microbiology, College of Medicine, Taif University

References

• Abdelmenan S., Teka H., Hwang J., Girma S., Chibsa S., Tongren E., Murphy M., Haile M., Dillu D., Kassim J., Behaksra S., Tadesse F. G., Yukich J., Berhane Y., Worku A., Keating J., Zewde A., Gadisa E. (2022) Evaluation of the effect of targeted Mass Drug Administration and Reactive Case Detection on malaria transmission and elimination in Eastern Hararghe zone, Oromia, Ethiopia: A cluster randomized control trial. Trials 23: 267-278
• Antinori S., Ridolfo A. L., Grande R., Galimberti L., Casalini G., Giacomelli A., Milazzo L. (2021) Loop-mediated isothermal amplification (LAMP) assay for the diagnosis of imported malaria: A narrative review. Infez. Med. 29: 355-365
• Charpentier E., Benichou E., Pagès A., Chauvin P., Fillaux J., Valentin A., Guegan H., Guemas E., Salabert A.-S., Armengol C., Menard S., Cassaing S., Berry A., Iriart X. (2020) Performance evaluation of different strategies based on microscopy techniques, rapid diagnostic test and molecular loop-mediated isothermal amplification assay for the diagnosis of imported malaria. Clin. Microbiol. Infect. 26: 115-121
• Dharmaratne A. D. V. T. T., Dini S., O’Flaherty K., Price D. J., Beeson J., McGready R., Nosten F., Fowkes F. J. I., Simpson J. A., Zaloumis S.G. (2022) Quantification of the dynamics of antibody response to malaria to inform sero-surveillance in pregnant women. Malar. J. 21: 75-87
• Gardner M. J., Hall N., Fung E., White O., Berriman M., Hyman R. W., Carlton J. M., Pain A., Nelson K. E., Bowman S., Paulsen I. T., James K., Eisen J. A., Rutherford K., Salzberg S. L., Craig A., Kyes S., Chan M.-S., Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M. W., Vaidya A. B., Martin D. M. A., Fairlamb A. H., Fraunholz M. J., Roos D. S., Ralph S. A., McFadden G. I., Cummings L. M., Subramanian G. M., Mungall C., Venter J. C., Carucci D. J., Hoffman S. L., Newbold C., Davis R. W., Fraser C. M., Barrell B. (2002) Genome sequence of the human malaria parasite Plasmodium falciparum. Nature 419: 14-48
• Gruenberg M., Moniz C. A., Hofmann N. E., Koepfli C., Robinson L. J., Nate E., Monteiro W. M., de Melo G. C., Kuehn A., Siqueira A. M., Nguitragool W., Bassat Q., Lacerda M., Sattabongkot J., Mueller I., Felger I. (2020) Utility of ultra+sensitive qPCR to detect Plasmodium falciparum and Plasmodium vivax infections under different transmission intensities. Malar. J. 19: 319-328
• Gruenberg M., Moniz C. A., Hofmann N. E., Wampfler R., Koepfli C., Mueller I., Monteiro W. M., Lacerda M., de Melo G. C., Kuehn A., Siqueira A. M., Felger I. (2018) Plasmodium vivax molecular diagnostics in community surveys: Pitfalls and solutions. Malar. J. 17: 55-64
• Han E.-T., Watanabe R., Sattabongkot J., Khuntirat B., Sirichaisinthop J., Iriko H., Jin L., Takeo S., Tsuboi T. (2007) Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J. Clin. Microbiol. 45: 2521-2528
• Hofmann N., Mwingira F., Shekalaghe S., Robinson L. J., Mueller I., Felger I. (2015) Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets. PLOS Med. 12: e1001788
• Hofmann N. E., Gruenberg M., Nate E., Ura A., Rodriguez-Rodriguez D., Salib M., Mueller I., Smith T. A., Laman M., Robinson L. J., Felger I. (2018) Assessment of ultra-sensitive malaria diagnosis versus standard molecular diagnostics for malaria elimination: An in+depth molecular community cross-sectional study. Lancet Infect. Dis. 18: 1108-1116
• Kourtis A. P., Read J. S., Jamieson D. J. (2014) Pregnancy and infection. N. Engl. J. Med. 370: 2211-2218
• Lai M. Y., Ooi C. H., Jaimin J. J., Lau Y. L. (2020) Evaluation of WarmStart colorimetric loop-mediated isothermal amplification assay for diagnosis of malaria. Am. J. Trop. Med. Hyg. 102: 1370-1372
• Lucchi N. W., Ljolje D., Silva-Flannery L., Udhayakumar V. (2016) Use of malachite green-loop mediated isothermal amplification for detection of Plasmodium spp. parasites. PLOS ONE 11: e0151437
• Morris U., Aydin-Schmidt B. (2021) Performance and application of commercially available loop-mediated isothermal amplification (LAMP) kits in malaria endemic and non-endemic settings. Diagnostics 11: 336-353
• Nijhuis R. H. T., van Lieshout L., Verweij J. J., Claas E. C. J., Wessels E. (2018) Multiplex real-time PCR for diagnosing malaria in a non-endemic setting: A prospective comparison to conventional methods. Eur. J. Clin. Microbiol. Infect. Dis. 37: 2323-2329
• Okell L. C., Bousema T., Griffin J. T., Ouédraogo A. L., Ghani A. C., Drakeley C. J. (2012) Factors determining the occurrence of submicroscopic malaria infections and their relevance for control. Nat. Commun. 3: 1237-1245
• Orish V. N., De-Gaulle V. F., Sanyaolu A. O. (2018) Interpreting rapid diagnostic test (RDT) for Plasmodium falciparum. BMC Res. Notes 11: 850-855
• Picot S., Cucherat M., Bienvenu A.-L. (2020) Systematic review and meta-analysis of diagnostic accuracy of loop-mediated isothermal amplification (LAMP) methods compared with microscopy, polymerase chain reaction and rapid diagnostic tests for malaria diagnosis. Int. J. Infect. Dis. 98: 408-419
• Selvarajah D., Naing C., Htet N. H., Mak J. W. (2020) Loop-mediated isothermal amplification (LAMP) test for diagnosis of uncomplicated malaria in endemic areas: A meta-analysis of diagnostic test accuracy. Malar. J. 19: 211-220
• Soliman R. H., Garcia-Aranda P., Elzagawy S. M., Hussein B. E.-S., Mayah W. W., Martin Ramirez A., Ta-Tang T.-H., Rubio J. M. (2018) Imported and autochthonous malaria in West Saudi Arabia: Results from a reference hospital. Malar. J. 17: 286-292
• Vernelen K., Barbé B., Gillet P., Van Esbroeck M., China B., Jacobs J. (2018) Photo-based External Quality Assessment of Malaria rapid diagnostic tests in a non-endemic setting. PLOS ONE 13: e0201622
• WHO (2021) Malaria report

Identifiers

DOI

10.4467/16890027AP.23.002.17903

Biblioteka Nauki

51992733