Morphological and Molecular Identification of Isospora sepetibensis (Chromista: Miozoa: Eimeriidae) from a New Host, Trichothraupis melanops (Passeriformes: Thraupidae: Tachyphoninae) in South America

• Authors: Jhon Lennon Genovez-Oliveira , Sergian V. Cardozo , Águida A. De Oliveira , Viviane M. De Lima , Ildemar Ferreira , Bruno P. Berto

Abstracts

EN

Isospora sepetibensis Berto, Flausino, Luz, Ferreira and Lopes, 2008 is a protozoan coccidian parasite (Chromista: Miozoa: Coccidiomorphea: Coccidia) that was originally described from Brazilian tanagers Ramphocelus bresilius (Linnaeus, 1766) in the Marambaia Island in the Coast of the State of Rio de Janeiro. In the current  work, this species was identified from black-goggled tanagers Trichothraupis melanops (Vieillot, 1818) in the Itatiaia National Park, which is a protected area with a high degree of vulnerability in the interior of the State of Rio de Janeiro, distant in more than 100 km of the type-locality. Its oocysts are sub-spherical to elongate  ovoidal, 25.9 × 20.7 μm with smooth, bi-layered wall, ~ 1.3 μm and length/width ratio of 1.1–1.4 (1.26). Micropyle and oocyst residuum absent, but one or two polar granules are present. Sporocysts are ellipsoidal, 16.8 × 10.3 μm, with both Stieda and sub-Stieda bodies. Sporocyst residuum present  and sporozoites with refractile body and nucleus. Molecular analysis was conducted at the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. This new isolate exhibited similarity greater than 98% with Isospora spp. isolates from other Neotropical passerines and with an Isospora sp. pseudoparasite of voles of Eurasia. This is the first coccidian  parasite from a New World tanager to have a molecular identification of the cox1 gene.

Keywords

EN

Coccidia; oocysts; Neotropical birds; Thraupidae; morphology; molecular biology; taxonomy; phylogeny; Parque Nacional do Itatiaia

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2019
• Volume: 58
• Issue: 1
• Pages: 17-23

Dates

published

2019

Contributors

author

Jhon Lennon Genovez-Oliveira

• Programa de Pós-Graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro (UFRRJ), Brazil

author

Sergian V. Cardozo

• Programa de Pós-Graduação em Biomedicina Translacional, Universidade do Grande Rio, RJ, Brazil

author

Águida A. De Oliveira

• Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

author

Viviane M. De Lima

• Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

author

Ildemar Ferreira

• Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

author

Bruno P. Berto

• Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, UFRRJ, RJ, Brazil

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Identifiers

DOI

10.4467/16890027AP.19.007.10838

Biblioteka Nauki

52073923