Systematic Studies on the Hypotrich Ciliate, Tachysoma pellionellum (Müller, 1773) Borror, 1972 (Protozoa, Ciliophora) Based on Integrative Analyses: Morphology, Morphogenesis and Molecular Phylogeny

• Authors: Lingyun Chen , Xiaolu Zhao , Hamed A. El-Serehy , Jie Huang , John C. Clamp

Abstracts

EN

Tachysoma pellionellum Stokes, 1887, a freshwater ciliate isolated from Stone Mountain State Park, North Carolina, was studied in vivo and after staining with protargol. The population was characterized mainly by having the typical 18 frontal-ventral-transverse cirri; posterior ends of left and right marginal rows not confluent; five dorsal kineties and one dorsomarginal kinety; two macronuclear nodules near left cell margin with one or two micronuclei between them; contractile vacuole located at mid-body near left margin. Morphogenesis is characterized as follows: (1) in the proter, the parental adoral zone of membranelles is retained completely; (2) 18 frontal-ventral-transverse cirri are derived from the anlage of the undulating membrane and the five streaks of the frontal-ventral-transverse anlagen; (3) marginal rows develop intrakinetally; (4) anlagen of dorsal kineties 1, 2 and 4 develop in the parental structure and anlagen of dorsal kineties 2 and 4 fragment in the posterior region forming anlagen of dorsal kineties 3 and 5; (5) only one dorsomarginal kinety formed; (6) the two macronuclear nodules fuse into a single mass, which then divides. Phylogenetic analyses based on sequences of the gene coding for SSU RNA revealed a close relationship between T. pellionellum and the Oxytricha clade, both of which grouped with Kleinstyla dorsicirrata and Heterourosomoida lanceolata.

Keywords

EN

fresh water ciliate; ontogenesis; phylogenetic analysis; taxonomy

• Publisher: Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
• Journal: Acta Protozoologica
• Year: 2017
• Volume: 56
• Issue: 4
• Pages: 221-233

Dates

published

2017

Contributors

author

Lingyun Chen

• College of Life Science, Northwest Normal University, Lanzhou, China
• Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China

author

Xiaolu Zhao

• Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao, China

author

Hamed A. El-Serehy

• Department of Zoology, King Saud University, Riyadh, Saudi Arabia

author

Jie Huang

• Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China

author

John C. Clamp

• Department of Biological and Biomedical Sciences, North Carolina Central University, Durham, North Carolina, USA

References

• Berger H. (1999) Monograph of the Oxytrichidae (Ciliophora, Hypotrichia). Monogr. Biol. 78: 1–1080
• Berger H. (2006) Monograph of the Urostyloidea (Ciliophora, Hypotricha). Monogr. Biol. 85: 1–1304
• Berger H. (2008) Monograph of the Amphisiellidae and Trachelostylidae (Ciliophora, Hypotricha). Monogr. Biol. 88: 1–737
• Berger H. (2011) Monograph of the Gonostomatidae and Kahliellidae (Ciliophora, Hypotricha). Monogr. Biol. 90: 1–741
• Bharti D., Kumar S., Terza, A. L. (2016) Rigidosticha italiensis n. gen., n. sp. (Ciliophora, Spirotricha), novel large hypotrich ciliate from the soil of Lombardia, Italy. Eur. J. Protistol. 56: 112–118
• Bharti D., Kumar S., Terza A. L. (2017) Description and molecular phylogeny of a novel hypotrich ciliate from the soil of Marche Region, Italy; including notes on the MOSYSS project. J. Eukaryot. Microbiol. J. Eukaryot. Microbiol. 64: 678–690
• Chen L., Zhao X., Shao C., Miao M., Clamp J. C. (2017) Morphology and phylogeny of two new ciliates Sterkiella sinica sp. nov. and Rubrioxytricha tsinlingensis sp. nov. (Protozoa, Ciliophora, Hypotrichia) from north-west China. Syst. Biodivers. 15: 131–142
• Foissner W. (2016) Terrestrial and semiterrestrial ciliates (Protozoa, Ciliophora) from Venezuela and Galápagos. Denisia 35: 1–912
• Foissner W., Agatha S., Berger H. (2002) Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Part I. Text and line drawings. Part II: Photographs. Denisia 5: 1–1459
• Gao F., Warren A., Zhang Q., Gong J., Miao M., Sun P., Xu D., Huang J., Yi Z., Song W. (2016). The all-data-based evolutionary hypothesis of ciliated protists with a revised classification of classificationof the phylum Ciliophora (Eukaryota, Alveolata). Sci. Rep. 6, 24874
• Hall T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41: 95–98
• Hemberger H. (1982) Revision der Ordnung Hypotrichida Stein (Ciliophora, Protozoa) an Hand von Protargolpräparaten und Morphogenesedarstellungen. PhD Thesis, University of Bonn, Germany
• Huang J., Luo X., Bourland W. A., Gao F., Gao S. (2016) Multigene-based phylogeny of the ciliate families Amphisiellidae and Trachelostylidae (Protozoa: Ciliophora: Hypotrichia). Mol. Phylogenet. Evol. 101: 101–110
• Kumar S., Bharti D., Quintela-Alonso P., Shin M. K., Terza A. L. (2016) Fine-tune investigations on three stylonychid (Ciliophora, Hypotricha) ciliates. Eur. J. Protistol. 56: 200–218
• Kumar S., Foissner W. (2017) Morphology and ontogenesis of Stylonychia (Metastylonychia) nodulinucleata nov. subgen. (Ciliophora, Hypotricha) from Australia. Eur. J. Protistol. 57: 61–72
• Li F., Lyu Z., Li Y., Fan X., Al-Farraj S. A., Shao C., Berger H. (2017) Morphology, morphogenesis, and molecular phylogeny of Uroleptus (Caudiholosticha) stueberi (Foissner, 1987) comb. nov. (Ciliophora, Hypotricha), and reclassification of the remaining Caudiholosticha species. Eur. J. Protistol. 59: 82–98
• Li F., Xing Y., Li J., Al-Rasheid K. A. S., He S., Shao C. (2013) Morphology, morphogenesis and small subunit rRNA gene sequence of a soil hypotrichous ciliate, Perisincirra paucicirrata (Ciliophora, Kahliellidae), from the shoreline of the Yellow River, North China. J. Eukaryot. Microbiol. 60: 247–256
• Lu X. T., Huang J., Shao C., Al-Farraj S. A., Gao S. (2017) Morphology and morphogenesis of a novel saline soil hypotrichous ciliate, Gonostomum sinicum nov. spec. (Ciliophora, Hypotrichia, Gonostomatidae), including a report on the small subunit rDNA sequence. J. Eukaryot. Microbiol. 64: 32–646
• Luo X. T., Gao F., Yi Z., Pan Y., Al-Farraj S. A., Warren A. (2017) Taxonomy and molecular phylogeny of two new brackish hypotrichous ciliates, with the establishment of a new genus (Ciliophora, Spirotrichea). Zool. J. Linn. Soc. 179: 475–491
• Medlin L., Elwood H. J., Stickel S., Sogin M. L. (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71: 491–499
• Müller O. F. (1773) Vermium terrestrium et fluviatilium, seu animalium infusorium, helminthicorum et testaceorum, non marinorum, succincta historia. Havniae and Lipsiae.
• Nylander J. A. A. (2004) MrModeltest, version 2.2. Evolutionary biology centre, Uppsala University, Uppsala
• Page R. D. M. (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Comput. Appl. Biosci. 12: 357–358
• Park M. H., Jung J. H., Min G. S., Kim S. (2017a) Pseudonotohymena antarctica n. g., n. sp. (Ciliophora, Hypotricha), a new species from Antarctic soil. J. Eukaryot. Microbiol. 64: 447–456
• Park M. H., Moon J. H., Kim K. N., Jung J. H. (2017b) Morphology, morphogenesis, and molecular phylogeny of Pleurotricha oligocirrata nov. spec. (Ciliophora: Spirotrichea: Stylonychinae). Eur. J. Protistol. 59: 114–123
• Ronquist F., Huelsenbeck J. P. (2003) MrBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574
• Schmidt S. L., Bernhard D., Schlegel M., Foissner W. (2007) Phylogeny of the Stichotrichia (Ciliophora; Spirotrichea) reconstructed with nuclear small subunit rRNA gene sequences: discrepancies and accordances with morphological data. J. Eukaryot. Microbiol. 54: 201–209
• Shao C., Gao S., Hu X., Al-Rasheid K. A., Warren A. (2011) Ontogenesis and molecular phylogeny of a new marine urostylid ciliate, Anteholosticha petzi n. sp. (Ciliophora, Urostylida). J. Eukaryot. Microbiol. 58: 254–265
• Shao C., Hu X., Warren A., Al-Rasheid K. A. S., Al-Quraishy S. A., Song W. (2007) Morphogenesis in the marine spirotrichous ciliate Apokeronopsis crassa (Claparède & Lachmann, 1858) n. comb. (Ciliophora: Stichotrichia), with the establishment of a new genus, Apokeronopsis n. g., and redefinition of the genus Thigmokeronopsis. J. Eukaryot. Microbiol. 54: 392–401
• Shao C., Lu X., Ma H. (2015) General overview of the typical 18 frontal-ventral-transverse cirri Oxytrichidae s. l. genera (Ciliophora, Hypotrichia). J. Ocean Univ. China. 14: 522–532
• Shao C., Pan X., Jiang J., Ma H., Al-Rasheid K. A. S., Warren A., Lin X. (2013) A redescription of the oxytrichid Tetmemena pustulata (Müller, 1786) Eigner, 1999 and notes on morphogenesis in the marine urostylid Metaurostylopsis salina Lei et al., 2005 (Ciliophora, Hypotrichia). Eur. J. Protistol. 49: 272–282
• Shao C., Song W., Al-Rasheid K. A. S., Berger H. (2012) Redefinition and reassignment of the 18-cirri genera Hemigastrostyla, Oxytricha, Urosomoida, and Actinotricha (Ciliophora, Hypotricha), and description of one new genus and two new species. Acta Protozool. 50: 263–287
• Song W., Warren A., Hu X. (2009) Free-living ciliates in the Bohai and Yellow Seas, China. Science Press, Beijing
• Stokes A. C. (1887) Some new hypotrichous infusoria from American fresh waters. Ann. Mag. nat. Hist., Serie 5 20: 104–114, Plate III
• Tamura K., Dudley J., Nei M., Kumar S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software Ver. 4.0. Mol. Biol. Evol. 24: 1596–1599
• Tchang T., Pang Y., Gu F. (1984) Evolution of nuclear apparatus and ciliatures of Tachysoma pellionella during asexual reproducing cycle. Zool. Res. 5: 189–200
• Wallengren H. (1900) Zur Kenntnis der vergleichenden Morphologie der hypotrichen Infusorien. Bih. K. Svenska Vetensk Akad. Handl. 26: 1–31
• Wang J., Lyu Z., Warren A., Wang F., Shao C. (2016) Morphology, ontogeny and molecular phylogeny of a novel saline soil ciliate, Urosomoida paragiliformis n. sp. (Ciliophora, Hypotrichia). Eur. J. Protistol. 56: 79–89
• Wang J., Li L. F., Warren A., Shao C. (2017) Morphogenesis and molecular phylogeny of the soil ciliate Rigidohymena quadrinucleata (Dragesco and Njine, 1971) Berger, 2011 (Ciliophora, Hypotricha, Oxytrichidae). Eur. J. Protistol. 60: 1–12
• Wilbert N. (1975) Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos 64: 171–179
• Zhao X., Gao S., Fan Y., Strueder-Kypke M., Huang J. (2015) Phylogenetic framework of the systematically confused Anteholosticha-Holosticha complex (Ciliophora, Hypotrichia) based on multigene analysis. Mol. Phylogenet. Evol. 91: 238–247

Identifiers

DOI

10.4467/16890027AP.17.020.7822

Biblioteka Nauki

52405188