Determination of replication initiation site

• Authors: J. Zakrzewska-Czerwinska , P. Mackiewicz , A. Zawilak , S. Cebrat
• Languages of publication: EN

Abstracts

EN

Initiation of bacterial chromosome replication is mediated by a single initiator protein ? DnaA which interacts specifically with multiple DnaA boxes located within the origin of replication oriC. We have applied in silico methods: DNA asymmetry, DnaA box distribution and dnaA gene location to identify the putative replication origins in bacterial chromosomes. The three methods identify the same region as a putative origin in more than half of the analyzed chromosomes. The most universal method of putative oriC identification in bacterial chromosomes is DNA asymmetry, although in some cases it is necessary to apply all three methods. Interestingly, most bacterial chromosomes exhibit an overrepresentation of DnaA boxes; they contain at least one cluster of DnaA boxes in the vicinity of the oriC region that is probably involved in controlling replication initiation.

Keywords

EN

BACTERIAL CHROMOSOME; DNA ASYMMETRY; REPLICATION

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2005
• Issue: 3
• Pages: 91-101

Contributors

author

J. Zakrzewska-Czerwinska

author

P. Mackiewicz

author

A. Zawilak

author

S. Cebrat

• Document Type: ARTICLE
• Publication order reference: Jolanta Zakrzewska-Czerwinska, Zaklad Mikrobiologii, Instytut Immunologii i Terapii Doswiadczalnej im. L. Hirszfelda, Polska Akademia Nauk, ul. Weigla 12, 53-114 Wroclaw, Poland