Influence of anther pretreatment on the efficiency of androgenesis in barley

• Authors: H. Kruczkowska , H. Pawlowska , B. Skucinska
• Languages of publication: EN

Abstracts

EN

The change in the developmental pathway of microspores from gametophytic to sporophytic is induced by stress during pretreatment of spikes and anthers. In our experiments, anther culture of three barley cultivars was tested with regard to the effect of chilling at 4?C for 28 days, starvation in 0.3 M mannitol solution for 4 days, and a combination of both methods. Chilling was shown to increase embryo/callus formation, while mannitol treatment favoured plant development, including development of green plants; simultaneous application of the two stress factors for 4 days proved to be ineffective. The tested cultivars exhibited a similar ability (calculated per 100 transferred embryos/calli) to develop plants without pretreatment; however, their responses to stress varied greatly. The collected data indicate that mannitol pretreatment, as compared to chilling, is more efficient in responsive cultivars.

Keywords

EN

ANDROGENESIS; BARLEY; CHILLING; STRESS

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2002
• Volume: 43
• Issue: 3
• Pages: 287-296

Contributors

author

H. Kruczkowska

author

H. Pawlowska

author

B. Skucinska

• Document Type: ARTICLE
• Publication order reference: H. Kruczkowska, Department of Plant Breeding and Seed Science, Agricultural University, ul. Lobzowska 24, 31-140 Krakow, Poland