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1997 | 38 | 4 | 471-476

Article title

Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

Title variants

Languages of publication

EN

Abstracts

EN
A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.

Keywords

Discipline

Year

Volume

38

Issue

4

Pages

471-476

Physical description

Contributors

author
author

References

Document Type

article

Publication order reference

S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, Oczapowskiego 5/30, 10-718 Olsztyn, Poland

Identifiers

YADDA identifier

bwmeta1.element.element-from-psjc-f9bab2e3-170e-3d9c-bb7c-7d826e1627d8
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