Genotyping of bovine beta-lactoglobulin (LGB) by PCR-SSCP technique

• Authors: S. Kaminski , T. Zabolewicz
• Languages of publication: EN

Abstracts

EN

A new method facilitating the identification of the two most common alleles (A and B) of the bovine beta-lacoglobulin (LGB) gene is described. The method is based on two steps: PCR amplification of 240 bp fragment of LGB gene followed by the single stranded conformation polymorphism (SSCP) detection. AA, AB and BB genotypes of LGB were identified with this technique. The PCR-SSCP is simple, accurate and relatively inexpensive. Additionally, this method has a potential to detect new variants within the amplified gene fragment.

Keywords

EN

BETA-LACTOGLOBULIN; CATTLE; GENOTYPING; PCR

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 4
• Pages: 471-476

Contributors

author

S. Kaminski

author

T. Zabolewicz

• Document Type: article
• Publication order reference: S.Kaminski, Department of Animal Genetics, University of Agriculture and Technology, Oczapowskiego 5/30, 10-718 Olsztyn, Poland