Calcium signal transduction in reconstructed oocytes and somatic cell nuclear transfer embryos of mammals in the conditions of artificial activation

• Authors: M. Samiec
• Languages of publication: EN

Abstracts

EN

One of the most important factors that affect the developmental competences of mammalian somatic cell nuclear transfer (SCNT) embryos is artificial activation of reconstructed oocytes (clonal cybrids). However, calcium signal transduction in a cytosol of such oocytes that has been initiated incorrectly by physical or chemical activating factors (electric pulses or specific ionophore antibiotics) can stimulate not only the development of cloned embryos. It can also induce apoptotic cell death following considerable elevation in intracellular calcium concentration and thereby excitotoxicity of Ca2+ ions. Therefore, the basic objective of this paper is to present the current knowledge on the mechanisms regulating biochemical and biophysical proapoptotic changes within SCNT embryos via the process of excitotoxic calcium signal transmission resulting from an improper artificial activation of clonal cybrid.

Keywords

EN

ACTIVATION; APOPTOSIS; CABP; CALCIUM; CALCIUM-RELEASE CHANNEL; IONOTROPIC RECEPTOR; RECONSTRUCTED OOCYTE; RYANODIN

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2010
• Issue: 1
• Pages: 46-65

Contributors

author

M. Samiec

• Document Type: REVIEW
• Publication order reference: Marcin Samiec, Dzial Biotechnologii Rozrodu Zwierzat, Instytut Zootechniki - Panstwowy Instytut Badawczy, Balice k. Krakowa, Poland