Analysis of plum (Prunus domestica L.) DNA polymorphism using RAPD - PCR technique

• Authors: A. Lisek , M. Korbin , E. Zurawicz , E. Rozpara
• Languages of publication: PL

Abstracts

EN

Precise identification of plum cultivars is desired in breeding programme of the species as well as in orchard practise. The aim of the presented studies was the determination of 19 plum cultivars Prunus domestica L. diversity. RAPD-PCR technique with 77 primers (Operon Technologies) from kits OPB, OPG, OPT and OPU was used for the analysis. Fifty-five polymorphic fragments DNA (600-2700 bp) were obtained in reactions with 33 primers. The highest number of polymorphic fragments (3-5) was observed in reactions with primers OPB 07, OPB 18, OPG 09, OPG 10 and OPT 14. The reactions with OPB 07, OPB 18 and OPG 09 allowed to diversify 15 cultivars, except 'Wegierka Zwykla' and clones 'Promis', 'Tolar', 'Nectavit'.

Keywords

EN

CULTIVAR; DNA POLYMORPHISM; MOLECULAR MARKER; PLUM; RAPD

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2004
• Issue: 2
• Pages: 76-72

Contributors

author

A. Lisek

author

M. Korbin

author

E. Zurawicz

author

E. Rozpara

• Document Type: ARTICLE
• Publication order reference: A. Lisek, Instytut Sadownictwa i Kwiaciarstwa, ul. Pomologiczna 18, 96-100 Skierniewice, Poland