Novel 12-bp deletion in the coding region of the bovine NPM1 gene affects growth traits

• Authors: Y.-Z. Huang , E.-P. Zhang , H. Chen , J. Wang , Z.-J. Li , Y.-T. Huai , L. Ma , X.-Y. Lan , G. Ren , C.-Z. Lei , X.-T. Fang , J.-Q. Wang
• Languages of publication: EN

Abstracts

EN

The nucleophosmin 1 gene (NPM1) encodes a multifunctional nucleolar phosphoprotein that plays a crucial role in the control of various aspects of cell growth and homeostasis. In this study, the coding region of the NPM1 gene was screened in 1035 individuals of 4 Chinese cattle breeds by DNA sequencing and polyacrylamide gel electrophoresis. A novel 12-bp deletion mutation was identified in the coding region of the NPM1 gene. The PCR products of primer NPM1-P2 exhibited 3 genotypes and 2 alleles: 178 bp (denoted as W) and 166 bp (denoted as D). Genotype DD and allele D were predominant in the studied populations. Association analysis with growth traits in the Nanyang breed (N = 265) showed that the animals with genotype DD had significantly greater birth weight, body weight, body length, and heart girth than those with genotype WD (P < 0.01 or P < 0.05) at birth and after 6 months and 12 months, but not at 18 and 24 months of age. Results of this study suggest that the NPM1 gene is a candidate gene for growth traits in cattle.

Keywords

EN

CATTLE; DELETION; GROWTH TRAIT; MUTATION; NPM1 GENE

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2010
• Volume: 51
• Issue: 2
• Pages: 199-202

Contributors

author

Y.-Z. Huang

author

E.-P. Zhang

author

H. Chen

author

J. Wang

author

Z.-J. Li

author

Y.-T. Huai

author

L. Ma

author

X.-Y. Lan

author

G. Ren

author

C.-Z. Lei

author

X.-T. Fang

author

J.-Q. Wang

• Document Type: ARTICLE
• Publication order reference: H. Chen, No.22 Xinong Road, College of Animal Science and Technology, Northwest A&F University, Yanggling, Shaanxi 712100, China