Bacterial diversity in the rumen of Indian Surti buffalo (Bubalus bubalis), assessed by 16S rDNA analysis

• Authors: P.R. Pandya , K.M. Singh , S. Parnerkar , A.K. Tripathi , H.H. Mehta , D.N. Rank , R.K. Kothari , C.G. Joshi
• Languages of publication: EN

Abstracts

EN

Bacterial communities in buffalo rumen were characterized using a culture-independent approach for a pooled sample of rumen fluid from 3 adult Surti buffaloes. Buffalo rumen is likely to include species of various bacterial phyla, so 16S rDNA sequences were amplified and cloned from the sample. A total of 191 clones were sequenced and similarities to known 16S rDNA sequences were examined. About 62.82% sequences (120 clones) had >90% similarity to the 16S rDNA database sequences. Furthermore, about 34.03% of the sequences (65 clones) were 85?89% similar to 16S rDNA database sequences. For the remaining 3.14%, the similarity was lower than 85%. Phylogenetic analyses were also used to infer the makeup of bacterial communities in the rumen of Surti buffalo. As a result, we distinguished 42 operational taxonomic units (OTUs) based on unique 16S r DNA sequences: 19 OTUs affiliated to an unidentified group (45.23% of total OTUs), 11 OTUs of the phylum Firmicutes, also known as the low G+C group (26.19%), 7 OTUs of the Cytophaga-Flexibacter-Bacteroides phylum (16.66%), 4 OTUs of Spirochaetes (9.52%), and 1 OTU of Actinobacteria (2.38%). These include 10 single-clone OTUs, so Good's coverage (94.76%) of 16S rRNA libraries indicated that sequences identified in the libraries represent the majority of bacterial diversity present in rumen.

Keywords

EN

16S RDNA; 16S RRNA; BUBALUS BUBALIS; MOLECULAR DIVERSITY; RUMEN BACTERIA; SURTI BUFFALO

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2010
• Volume: 51
• Issue: 3
• Pages: 395-401

Contributors

author

P.R. Pandya

author

K.M. Singh

author

S. Parnerkar

author

A.K. Tripathi

author

H.H. Mehta

author

D.N. Rank

author

R.K. Kothari

author

C.G. Joshi

• Document Type: ARTICLE
• Publication order reference: P. R. Pandya, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, 388 001 Gujarat, India