Homology of DNA sequences encompassing the malignant hyperthermia mutation site in the human, porcine, and zebrine ryr1 gene

• Authors: P. Gronek , R. Slomski , D. Lisiecka , A. Plawski , K. Nuc , T. Banasiewicz

Abstracts

EN

The RYR1 gene encoding the Ca2+ channel of sarcoplasmic reticulum of human skeletal muscle has been cloned and its nucleotide sequence has been determined earlier. We have used the polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and sequencing analysis for human, porcine (Sus scrofa), and zebrine (Equus grevyi) ryanodine receptor (ryr1) gene. The fragment of exon 17 of the ryr1 gene was characterized by a high homology between all the analysed species (substitution of a nucleotide is underlined): porcine ryr1 1834GTG GCC GTG CGC TCC AAC CAA GAT CT1859 human RYR1 1831GTG GCC GTG CGC TCC AAC CAA GAT CT1856 zebrine ryr1 GTG GCC GTG CGC TCC AAC CAA GAC CT.

Keywords

EN

HOMOLOGY; RYR1 GENE

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1998
• Volume: 39
• Issue: 3
• Pages: 275-279

Contributors

author

P. Gronek

author

R. Slomski

author

D. Lisiecka

author

A. Plawski

author

K. Nuc

author

T. Banasiewicz

• Document Type: short communica
• Publication order reference: P. Gronek, Department of Pig Breeding and Production, Agricultural University of Poznan, ul. Wolynska 33, 60-637 Poznan, Poland.