Reconstitution of brain mitochondria inner membrane into planar lipid bilayer

• Authors: B. Kulawiak , P. Bednarczyk
• Languages of publication: EN

Abstracts

EN

Ion channels are present in the inner mitochondrial membrane. They play an important role in cellular processes. Potassium and chloride channels are involved in regulation of mitochondrial volume, membrane potential and acidification. The mitochondrial potassium channels have been suggested as triggers and end effectors in cytoprotection. In our study we measured single channel activities after reconstitution of submitochondrial particles from rat brain mitochondria into planar lipid membranes. After incorporation, two different potassium selective currents were recorded with single channel conductance from 260 to 320 pS and from 70 to 90 pS in gradient (cis/trans) 50/450 and 50/150 mM KC1 solutions, respectively. We also observed activity of the chloride ion channel. The measured single channel conductance was from 80 to 90 pS in gradient {cis/trans) 50/450 mM KC1 solution. Our results suggest that various ion channels are present in the inner mitochondrial membrane of brain mitochondria.

Keywords

EN

BLACK LIPID MEMBRANE; BRAIN; CHLORIDE CHANNEL; MITOCHONDRIA; POTASSIUM CHANNEL; RAT

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2005
• Volume: 65
• Issue: 3
• Pages: 271-276

Contributors

author

B. Kulawiak

author

P. Bednarczyk

• Document Type: ARTICLE
• Publication order reference: Piotr Bednarczyk, Department of Biophysics, Agricultural University SGGW, 159 Nowoursynowska St., 02-776 Warsaw, Poland