Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale

• Authors: Jarzina A. Slusarkiewicz , A. Ponitka
• Languages of publication: EN

Abstracts

EN

Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (CHUANG et al. 1978) with increased 2,4 D to 2 mg L 1 and agarose 6 g L 1; 2 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 90 g L 1 sucrose; 3 - Macro , micronutrients and vitamines like in MN6 (CHU, HILL, 1988) + 2 mg L 1 2.4 D + 0.5 mg L 1 KIN + 5 mg L 1 FeEDTA + 120 g L 1 sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64 1250, 12 486 and 6 212 somatic embryos and 8 86, 3 136 and 1 26 green plants were recorded, on media 1, 2 and 3, respectively.

Keywords

EN

ANTHER CULTURE; HAPLOID PLANT; SOMATIC EMBRYO; TRITICALE

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 1997
• Volume: 38
• Issue: 3
• Pages: 253-258

Contributors

author

Jarzina A. Slusarkiewicz

author

A. Ponitka

• Document Type: article
• Publication order reference: A.Slusarkiewicz Jarzina, Institute of Plant Genetics, Polish Academy of Sciences, 60-479 Poznan, Strzeszynska 34, Poland