The effects of 2,4-D, NAA and picloram on callus induction and somatic embryogenesis in ornamental asparagus

• Authors: J. Kepczynski , S. Zielinska , E. Kepczynska
• Languages of publication: PL

Abstracts

EN

In this study we examined the effect of 2,4-D, NAA and picloram at 1.5, 3.0 and 4.5 mg/l in MS medium on callus induction and somatic embryogenesis in Asparagus densiflorus cv. Sprengeri and Asparagus plumosus. The callus formation of both shoot tips and nodal explants from A. densiflorus after 8 weeks of culture on MS medium supplemented with NAA and picloram (1.5, 3.0 mg/l) was observed. However, callus on medium containing 2,4-D was occasionally induced. On medium supplemented with picloram shoot tip explants of Asparagus plumosus showed callus inducing capacity. After four weeks of callus culture on hormone-free medium the globular embryos were achieved. On callus induced NAA organogenesis was observed.

Keywords

EN

AUXIN; CALLUS; GROWTH; ORNAMENTAL ASPARAGUS; SOMATIC EMBRYOGENESIS

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2001
• Issue: 2
• Pages: 170-173

Contributors

author

J. Kepczynski

author

S. Zielinska

author

E. Kepczynska

• Document Type: ARTICLE
• Publication order reference: J. Kepczynski, Katedra Fizjologii Roslin, Uniwersytet Szczecinski, Waska 13, 74-415 Szczecin, Poland