Bioconversion of trans-cinnamic acid to L-phenylalanine by Rhodotorula sp.

• Authors: I. Gientka , S. Blazejak , W Duszkiewicz-Reinhard
• Languages of publication: PL

Abstracts

EN

The synthesis of the aromatic amino acid L-phenylalanine has received considerable attention in recent years due to its increasing importance as precursor to the dipeptide sweetener aspartame. Phenylalanine ammonia lyase (PAL), which occurs in yeast, catalyzes the nonoxidative deamination of L-Phe to trans-cinnamic acid (tCA), has industrial application in the synthesis of L-Phe. Superior producers of PAL are Rhodotorula sp. PAL is induced in yeast cell by the presence of L-Phe, while glucose represses PAL synthesis. Different additives and conditions during inducing PAL: permeabilizing, reducing and stabilization agents, as well as pH and temperature during bioconversion targeting to higher productivity of L-Phe were discussed.

Keywords

EN

BIOCONVERSION; RHODOTORULA; TRANS-CINNAMIC ACID; YEAST

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2006
• Issue: 2
• Pages: 117-129

Contributors

author

I. Gientka

author

S. Blazejak

author

W Duszkiewicz-Reinhard

• Document Type: REVIEW
• Publication order reference: Iwona Gientka, Zaklad Biotechnologii i Mikrobiologii Zywnosci, Wydzial Technologii Zywnosci, Szkola Glowna Gospodarstwa Wiejskiego, ul. Nowoursynowska 159 c, 02-776 Warszawa, Poland