Gfp gene as a fluorescence tool in gene expression analysis and biosensors construction

• Authors: M. Matejczyk , S.J. Rosochacki
• Languages of publication: PL

Abstracts

EN

When autofluorescent protein such as green fluorescent protein (GFP) is excited with light of a specific wavelength, it emits light of a longer wavelength without further addition of substrates. Gfp belongs to a family of reporter genes which provide easily detectable phenotypes to microbial cells and are, therefore, a valuable tool for the study of microorganisms in the environment, especially for analysis of processes such as microbe-plant interactions, biofilm formation, horizontal gene transfer (HGT) and gene expression analysis in different conditions (under influence of biological, physical and chemical factors), and bacterial biosensors costruction. In this paper, the main possibilities of gfp gene as a marker application in microbial ecology, gene expression analysis and biosensors development are presented.

Keywords

EN

BIOSENSOR; GENE EXPRESSION; GFP GENE; GFP PROTEIN; REPORTER GENE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 2007
• Issue: 1
• Pages: 53-62

Contributors

author

M. Matejczyk

author

S.J. Rosochacki

• Document Type: REVIEW
• Publication order reference: Marzena Matejczyk, Katedra Biologii Sanitarnej i Biotechnologii, Politechnika Bia?ostocka, ul. Wiejska 45 E, 15-351 Bia?ystok, Poland