Kinetin, BAP and NAA increased glucotropaeolin content in dry weight of nasturtium hairy roots but they inhibited biomass growth. Salicylates most strongly (by about 100-200% above control) and DL-?-aminobutyric acid and methyl jasmonate to a lesser degree increased glucotropaeolin yield. They also slightly increased myrosinase activity. Trans-cinnamic acid was a much stronger inducer of myrosinase activity than glucotropaeolin biosynthesis: it strongly inhibited biomass growth. L-1-amino-2-phenylethylphosphonic acid, inhibitor of L-phenylalanine ammonia-lyase (PAL), induced glucotropaeolin production and enhanced the effect of salicylates and jasmonate on glucotropaeoplin yield but it did not affect myrosinase activity.