Isolation of low-molecular-weight glutenin subunit genes from wild emmer wheat (Triticum dicoccoides)

• Authors: Y.-W. Yue , H. Long , Q. Liu , Y.-M. Wei , Z.-H. Yan , Y.-L. Zheng
• Languages of publication: EN

Abstracts

EN

Three low-molecular-weight glutenin subunit (LMW-GS) genes, designated LMW-Td1, LMW-Td2 and LMW-Td3, were isolated from wild emmer wheat (Triticum dicoccoides), which is the tetraploid progenitor of common wheat (T. aestivum). The complete nucleotide sequence lengths of LMW-Td1, LMW-Td2 and LMW-Td3 are 858, 900 and 1062 bp, respectively. LMW-Td1 and LMW-Td3 can encode proteins with 284 and 352 amino acid residues, respectively, whereas LMW-Td2 is a putative pseudogene due to the presence of 3 inframe stop codons in its C-terminal domain. The deduced protein sequences of the 3 genes share the same typical polypeptide structures with known LMW-GS genes containing 8 cysteines in the mature protein domains. LMW-Td1 was clearly distinguished from all known LMW-GS genes, and considered as a novel LMW-GS gene. Two hydrophobic motifs (i.e. PIIIL and PVIIL) were observed in the repetitive domain of LMW-Td3. Sequence comparison indicates that sequences of the 3 LMW-GS genes from this study are strongly similar to known LMW-GS genes. Our phylogenetic analysis suggests that LMW-Td1 and LMW-Td2 are homologous with genes on chromosome 1A, and LMW-Td3 is closely related to genes on chromosome 1B.

Keywords

EN

EMMER WHEAT; LMW-GS GENE; SEQUENCE ANALYSIS

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2005
• Volume: 46
• Issue: 4
• Pages: 349-355

Contributors

author

Y.-W. Yue

author

H. Long

author

Q. Liu

author

Y.-M. Wei

author

Z.-H. Yan

author

Y.-L. Zheng

• Document Type: ARTICLE
• Publication order reference: Y.L. Zheng, Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan 611830, China