TNF-alpha priming effect on polymorphonuclear leukocyte reactive oxygen species generation and adhesion molecule expression in hemodialyzed patients

• Authors: J. Rysz , M. Banach , R.A. Stolarek , J. Pasnik , A. Cia?kowska-Rysz , L. Markuszewski , Z. Baj
• Languages of publication: EN

Abstracts

EN

Introduction: The study aimed to assess reactive oxygen species generation and the expressions of some surface antigens on polymorphonuclear leukocytes (PMNs) in patients on regular hemodialysis (HD) treatment. Materials and Methods: The respiratory burst of PMNs was determined with luminol-dependent chemiluminescence (CL) in resting cells and following N-formyl-methionyl-leucyl-phenylalanine (fMLP), phorbol 12-myristate 13-acetate (PMA), or opsonized zymosan (OZ) stimulation and expressed in arbitrary CL units times assay-time (aUmin). The expressions of CD11b/CD18, CD10, and CD13 receptors were determined with flow cytometry. Results: Basal PMN CL was increased in HD patients to up to 1285129 aUmin compared with 89588 aUmin in healthy controls (p<0.05). The CL of unprimed PMNs increased after fMLP stimulation from 3085746 to 4529808 aUmin, and after OZ stimulation from 129451296 to 146781355 aUmin. PMA-stimulated CL of PMNs was similar to control values. The oxidative burst in PMNs from HD patients and healthy controls was similar in response to TNF-alpha alone. The CL of TNF- alpha-primed PMNs in HD patients was significantly lower than CL measured in healthy controls (p<0.05). The expressions of CD10 and CD13 metalloproteinase receptors were also increased (p<0.05). Although CD11b expression was significantly increased at rest and after fMLP stimulation, the expression of another beta-integrin heterodimer compound, CD18, was not increased. Conclusions: These results provide evidence that TNF- priming of PMNs is down-regulated in HD patients despite constitutive up-regulation of resting cytotoxicity and enhanced expression of adhesion and metalloproteinase receptors.

Keywords

EN

CHEMILUMINESCENCE; HEMODIALYSIS; NEUTROPHIL; TNF-ALPHA

Discipline

• IMMUNOLOGY: IMMUNOLOGY

• Publisher: Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences
• Journal: Archivum Immunologiae et Therapiae Experimentalis
• Year: 2006
• Volume: 54
• Issue: 3
• Pages: 209-215

Contributors

author

J. Rysz

author

M. Banach

author

R.A. Stolarek

author

J. Pasnik

author

A. Cia?kowska-Rysz

author

L. Markuszewski

author

Z. Baj

• Document Type: ARTICLE
• Publication order reference: Jacek Rysz, 2nd Department of Family Medicine, Medical University of Lodz, Zeromskiego 113, 90-549 Lodz, Poland