Sex determination in 6 bovid species by duplex PCR

• Authors: Prashant. , D.S. Gour , P.P. Dubey , A. Jain , S.C. Gupta , B.K. Joshi , D. Kumar
• Languages of publication: EN

Abstracts

EN

Sex determination in domestic animals is of potential value to livestock breeding programs. The aim of this study was to develop a simple and accurate PCR-based sex determination protocol, which can be applicable to 6 major domesticated species of the family Bovidae, viz. Bos frontalis, B. grunniens, B. indicus, Bubalus bubalis, Capra hircus, and Ovis aries. In silico analysis was done to identify conserved DNA sequence in the HMG box region of the sex-determining region of the Y-chromosome (SRY gene) across the bovids. Duplex PCR assay, including the SRY gene and the GAPDH housekeeping gene, was optimized by using genomic DNA extracted from blood samples of known sex. It was possible to identify the sex of animals by amplifying both gender-specific (SRY) and autosomal (GAPDH) genes simultaneously in the duplex reaction, with the male yielding two bands and the female one band. The protocol was subjected to a blind test that showed a 100 percent specificity and accuracy, thus it can be used in sex determination in livestock breeding programs.

Keywords

EN

DUPLEX PCR; SEX DETERMINATION; SRY GENE

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2008
• Volume: 49
• Issue: 4
• Pages: 379-381

Contributors

author

Prashant.

author

D.S. Gour

author

P.P. Dubey

author

A. Jain

author

S.C. Gupta

author

B.K. Joshi

author

D. Kumar

• Document Type: ARTICLE
• Publication order reference: Prashant, Genes and Genetic Resources Molecular Analysis Lab, National Bureau of Animal Genetic Resources, Karnal ?132001, India