Short-chain oligosaccharide synthesis by endo-beta-1,3 gluconase from Oerskovia xanthineolytica

• Authors: S. Bielecki , A. Bukowiecka
• Languages of publication: EN

Abstracts

EN

An approach for enzymatic sythesis of oligosachcharides via transglycosylation with endo-beta-1,3-glucanase from Oerskovia xanthineolytica is described.Linear -beta-1,3-glucan (laminarin) was used as a donor of glycosyl residues, whereas nitrophenyl glycosides of different monosaccharides served as acceptors.The synthesis was performed in water-organic solvent environment with several combinations of donor/acceptor.Employing p-nitrophenyl-beta-D-xylopyranoside as an acceptor in the presence of 30% acetonitrile resulted in the production of six new glycosides shown by FAB MS to be di-, tri- and tetrasaccharides.This enzyme is therefore suitable for the synthesis of short-chain oligosaccharides.

Keywords

EN

ENDO-BETA-1,3-GLUCANASE; OERSKOVIA XANTHINEOLYTICA; OLIGOSACCHARIDE

Discipline

• BIOTECHNOLOGY: BIOTECHNOLOGY

• Publisher: Committee of Biotechnology, Polish Academy of Sciences
• Journal: Biotechnologia
• Year: 1996
• Issue: 2
• Pages: 184-191

Contributors

author

S. Bielecki

author

A. Bukowiecka

• Document Type: article
• Publication order reference: S.Bielecki