Isozyme and RAPD markers for the identification of pea, field bean and lupin cultivars

• Authors: B. Wolko , W.K. Swiecicki , K. Kruszka , L. Irzykowska
• Languages of publication: EN

Abstracts

EN

The limited gene pool used in breeding decreases the level of genetic diversity in legume cultivars. Morphological or physiological characters are not always sufficient for quick, easy and precise cultivar description. Isozyme variability of 33 commercial Polish pea cultivars was analysed. The level of allozyme polymorphism discovered was high enough for the identification of all cultivars within two groups: white flowering peas for human consumption and colored flowering peas for fodder. The range of RAPD marker polymorphism among three lupin crops (white lupin, narrow-leafed lupin and yellow lupin) was tested. It was possible to identify each of four white lupin cultivars by means of bands generated by two primers. Seven narrow-leafed lupin cultivars were distinguished using three other primers. Testing of RAPD marker polymorphism, supplemented in some cases with observations of seed coat color genes, allowed to identify all 12 yellow lupin cultivars. Thirteen field bean cultivars were tested by isozyme variability and RAPD polymorphism observations. Among 15 enzyme systems investigated, 10 showed a high level of inter- and intracultivar polymorphism but the range of allozyme variability discovered was useless for cultivar identification. All cultivars analysed could be distinguished by a combination of RAPD markers amplified using two primers.

Keywords

EN

CULTIVAR IDENTIFICATION; ISOZYME; LUPINUS ALBUS; LUPINUS ANGUSTIFOLIUS; LUPINUS LUTEUS; PISUM SATIVUM; VICIA FABA

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2000
• Volume: 41
• Issue: 3
• Pages: 151-165

Contributors

author

B. Wolko

author

W.K. Swiecicki

author

K. Kruszka

author

L. Irzykowska

• Document Type: article
• Publication order reference: B. Wolko, Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszynska 34, 60-479 Poznan, Poland, email: [e-mail].