Effects of Bacillus thuringiensis kurstaki on malpighian tubule cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae) larvae

• Authors: A. Ogutcu , Z. Suludere , M. Uzunhisarcikli , Y. Kalender
• Languages of publication: EN

Abstracts

EN

In this study effects of Bacillus thuringiensis kurstaki (Btk) on Malpighian tubule cells of Thaumetopoea pityocampa (Lepidoptera: Thaumetopoeidae) larvae was investigated by electron microscopy. 3 mg/l Btk was given with food. After Btk administration, the Malpighian tubule cells were investigated and compared with a control group. 3 and 6 hrs after Btk administration swelling in Malpighian tubule cells was observed. Swelling of mitochondria and separation of their cristae was seen after 12 hrs. After 24 hrs dissolution of the basal cytoplasm, swelling and vacuolization of all mitochondria, partial dissolution of the nucleoplasm, and swelling and separation of microvilli was documented. A membrane-body in the nucleus was seen after 48 hrs. The nucleoplasm was completely dissolved after 72 hrs and after 96 hrs large vacuoles appeared in the cytoplasm and shortening of microvilli was observed.

Keywords

EN

BACILLUS THURINGIENSIS KURSTAKI; MALPIGHIAN TUBULES; THAUMETOPOEA PITYOCAMPA; ULTRASTRUCTURE

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Institute of Systematics and Evolution of Animals, Polish Academy of Sciences
• Journal: Folia Biologica
• Year: 2005
• Volume: 53
• Issue: 1-2
• Pages: 7-11

Contributors

author

A. Ogutcu

author

Z. Suludere

author

M. Uzunhisarcikli

author

Y. Kalender

• Document Type: ARTICLE
• Publication order reference: Yusuf Kalender, Gazi University, Faculty of Arts and Science, Department of Biology, 06500 Teknikokullar Ankara, Turkey