Tools of the trade: diagnostics and research in domestic animal cytogenetics

• Authors: L Iannuzzi , D. Berardino_Di
• Languages of publication: EN

Abstracts

EN

This paper describes the most common cytogenetic techniques we routinely adopt in our laboratories for producing high-resolution banding on prometaphase stage chromosomes, from synchronized or nonsynchronized blood cultures. Special emphasis is given to the FISH procedures applied to prometaphase chromosomes for mapping purposes. Each section includes historical information, basic principles for the given technique, its primary use in veterinary cytogenetics, and major limitations. Supplementary material (protocols and chemicals used) are available on our website. Even though these techniques mainly refer to the Bovidae, they can be easily extended and adapted to members of other taxa.

Keywords

EN

ANIMAL; CHROMOSOME BANDING; CYTOGENETICS; FISH; TECHNIQUE

Discipline

• GENETICS: GENETICS

• Publisher: Institute of Plant Genetics, Polish Academy of Sciences
• Journal: Journal of Applied Genetics
• Year: 2008
• Volume: 49
• Issue: 4
• Pages: 357-366

Contributors

author

L Iannuzzi

author

D. Berardino_Di

• Document Type: ARTICLE
• Publication order reference: Dino Di Berardino, Department of Soil, Plant, Environment and Animal Production Sciences, University of Naples 'Federico II', 80055 Portici, Naples, Italy