Fragment C tetanus toxin: A putative activity-dependent neuroanatomical tracer

• Authors: F.J. Miana-Mena , M.J. Munoz , J. Ciriza , J. Soria , P. Brulet , P. Zaragoza , R. Osta
• Languages of publication: EN

Abstracts

EN

Abstract. The non-toxic C fragment of tetanus toxin fused to the b-galactosidase enzyme was analysed as a neuroanatomical tracer. After intramuscular injection in rat tongue, its location in the hypoglossal network was compared with other classic tracers such as neurotropic viruses. The hybrid protein reached second and higher-order neurons after crossing several synapses. It appears to be a powerful tool to map neuronal circuits since the protein is easy to handle and detect and its transsynaptic transport is potential activity-dependent.

Keywords

EN

B-GALACTOSIDASE; HYPOGLOSSAL NUCLEUS; NEUROANATOMICAL TRACER; TETANUS TOXIN

Discipline

• EXPERIMENTAL_BIOLOGY_&_MEDICINE: EXPERIMENTAL BIOLOGY & MEDICINE

• Publisher: Marceli Nencki Institute of Experimental Biology, Polish Academy of Sciences
• Journal: Acta Neurobiologiae Experimentalis
• Year: 2003
• Volume: 63
• Issue: 3
• Pages: 211-218

Contributors

author

F.J. Miana-Mena

author

M.J. Munoz

author

J. Ciriza

author

J. Soria

author

P. Brulet

author

P. Zaragoza

author

R. Osta

• Document Type: ARTICLE
• Publication order reference: F.J. Miana-Mena, Laboratorio de Genetica Bioquimica y Grupos Sanguineos, Facultad de Veterinaria de Zaragoza, C/ Miguel Servet 177, CP 50013, Zaragoza, Spain